1. Granzymes in impaired wound healing
The research project will focus on characterizing the role of granzymes in tissue injury and impaired wound healing pertaining to aging-related, chronic inflammatory conditions (including pressure injuries, diabetic wounds) using in vitro assays, in vivo disease models, and patient samples. Methodologies used may include: transcriptomics, proteomics, histology, immunohistochemistry, immunofluorescence, immunoblotting, protease assays, ELISAs, and cell culture. The student will have the opportunity to co-author research publications should significant findings arise from their contributions.
Research area, student roles & skills
Research area: The overarching theme of my research program is to better understand the mechanisms underlying tissue injury and wound healing in aging-related, chronic inflammatory conditions. Through phenotypic and molecular characterizations using in vitro studies, in vivo models, and patient biospecimens, our goal is to identify therapeutic targets that could be modulated to improve disease severity and clinical symptoms in patients. In particular, our team is interested in a family of serine proteases known as granzymes (granule-secreted enzymes). Over the past 20+ years, our team has discovered a pathogenic role for granzymes in many age-related, chronic inflammatory conditions/diseases in diverse tissues.
Student roles:
The student will be immersed in a wide range of translational research activities and will work with a Postdoctoral Fellow or Senior Graduate Student supervisor. Day to day tasks will include designing studies, running experiments, scientifically documenting methodology and results, analyzing and interpreting findings, discussing data with supervisors, organizing data into presentations, delivering presentations at lab meetings and local research meetings, writing and editing research manuscripts as well as assisting with other lab duties and studies.
Skills required:
We are looking for a highly-motivated, committed student with a passion for biomedical research and discovery. A background related to immunology, molecular biology, biochemistry, biology, physiology and/or physiology is preferred while previous hands-on experience with techniques such as tissue culture, protein/protease work, immunohistochemistry/histology is an asset. This work term is suitable for a senior student who has previous laboratory experience and is comfortable working independently and on a team. The laboratory is a dynamic, fast-paced, collaborative environment. Excellent written and oral communication skills are essential.