3D printing uses the specifications provided in a digital file to construct 3 dimensional objects and this process can be applied as a method to engineer tissues. The goal of this project is to use biomaterial-based bioinks to print functional 3D neural tissue from stem cells using 3D bioprinting. In particular, we wish to print tissues derived from human induced pluripotent stem cells (hiPSCs). hiPSCs are pluripotent stem cells produced by reprogramming adult cells back into a state where they can form any type of tissue. hiPSC lines derived from a patient could be printed into tissues used for screening potential treatments for their disease. Proper conditions must be met to ensure the printed hiPSCs form the desired type of tissue. During the printing process, cells are contained in "bioink", the material that surrounds the cells and enables them to be delivered in continuous streams. This project will bioprint tissues from hiPSCs and evaluate their properties. Such engineered tissues could serve as a tool for drug screening.
Research area, student roles & skills
Research area: My award winning, internationally recognized research group has generated a significant body of fundamental knowledge focused on 3D bioprinting human neural tissues using stem cells over the past 8 years. My research team has published 37 papers focused on 3D bioprinting, including 18 research papers, 16 reviews, and 3 invited commentaries, including one in Cell Stem Cell published in 2024 detailing how advances in bioprinting have made it possible to generate physiologically relevant human neural tissues on demand.
Student roles: The student will work with both the stem cells and biomaterial scaffolds to characterize their properties using a variety of techniques. In terms of the cell culture, the student will use microscopy, immunohistochemistry, real time PCR, and flow cytometry to characterize the differentiation state of the cells. The biomaterial scaffolds will be characterized using scanning electron microscopy as well as through release studies.
Skills required: Stem cell culture experience is preferred. Experience working with chemicals and biohazards along with experience immunohistochemistry, real time PCR, and flow cytometry would also be desirable.
2. A Transposable Element Expression Atlas of the Developing Human Brain
Transposable elements (TE) are mobile DNA sequences which have the ability to translocate to new locations throughout the genome. For some classes of TEs, translocation occurs through the expression of an RNA intermediate molecule which is reverse transcribed into DNA and inserted into a new location, generating many copies of the same sequence. Once thought to be largely repressed in humans TEs are actively expressed and mobile in the human brain particularly during brain development. Dysregulation of this activity has been implicated in several diseases including some types of cancers, schizophrenia and bi-polar disorder. However, at this point exploration of TE expression during brain development is limited.
The BRAINSPAN Atlas of the Developing Human Brain is a publicly available repository of transcriptomic, micro array and in situ hybridization datasets that span up to sixteen different brain regions across the full course of human brain development. This data has been used to map gene expression patterns across human brain development however very little work has been completed to explore TE expression patterns in this dataset.
This project aims to analyze the BRAINSPAN transcriptomic datasets for loci specific TE expression. We will utilize dedicated TE expression analysis programs to generate a TE expression map in the healthy human brain during development.
Research area, student roles & skills
Research area: My expertise is Bioinformatics and Machine Learning. My current research focuses on multi-omics analysis which provides novel information on the mechanisms of the biological process and cell states in disease development. We develop computational tools for complex and high-dimensional data including genome-wide population data, RNA-seq, and tandem mass spectra. I'm particularly interested in applying deep learning and AI to plants to advance digital agriculture.
Student roles: 1. Process and perform quality control on RNA-seq datasets from the BRAINSPAN Atlas of the Developing Human Brain using established bioinformatics pipelines
2. Implement and optimize dedicated TE expression analysis tools (e.g., TEtranscripts, Telescope) to quantify loci-specific transposable element expression
3. Generate a comprehensive TE expression map across multiple brain regions and developmental timepoints using the BRAINSPAN transcriptomic datasets
4. Address computational challenges associated with repetitive sequence mapping, including multi-mapping reads and TE polymorphisms
5. Conduct comparative expression analysis to identify developmental stage-specific and brain region-specific patterns of TE activity
6. Investigate associations between TE expression dynamics and known neurodevelopmental processes and disease-implicated genomic regions
7. Document findings through regular progress reports and lab meetings, and contribute to manuscript and figure preparation
8. Collaborate with supervisors and team members to integrate computational results within the broader context of brain development and TE biology
Skills required: 1. Strong foundation in molecular biology and genomics, including an understanding of DNA/RNA biology, transcription, and mobile genetic elements 2. Proficiency in bioinformatics and RNA-seq data analysis, including familiarity with transcriptomic pipelines and gene expression quantification tools 3. Computational skills including command-line (Linux/bash) and scripting experience in Python or R for data processing and visualization 4. Familiarity with sequence alignment, reference genomes, and the challenges associated with mapping repetitive or multi-mapping sequences 5. Experience or strong interest in working with large publicly available genomic datasets and high-performance computing environments
3. Antimicrobial resistance and host-pathogen interactions
Antimicrobial resistance is an emerging threat to human health worldwide and has reduced the treatment options for life-threatening bacterial infections, increased the burden of disease, and increased death rates. Chronic infections, which frequently involve biofilms, are especially difficult to treat due to the high antibiotic tolerance of biofilm cells. Oxidizing agents are low-molecular-weight molecules that oxidize other substances by accepting electrons from them. They include reactive oxygen species (ROS), such as hydrogen peroxide (H2O2), and reactive chlorine species (RCS) including sodium hypochlorite (NaOCl) and its active ingredient hypochlorous acid (HOCl). Bacteria encounter oxidizing agents in many different environments and from diverse sources. Among them, they are used as powerful disinfectants (e.g. household bleach NaOCl) and cleaning agents, but reactive oxygen and chlorine species are also produced by the human innate immune system to kill invading bacteria.
Emerging evidence suggests that targeting bacterial defense mechanisms such as antimicrobial resistance to reactive oxygen or chlorine species could lead to new therapies that assist the human immune system to kill pathogens. In this project, we want to investigate the molecular mechanism of antimicrobial resistance in biofilms formed by the opportunistic human pathogen Pseudomonas aeruginosa in order to fight chronic biofilm infections. To better understanding the molecular mechanisms underlying antimicrobial resistance of biofilms we will address the following two objectives:
1) Identify important genes and proteins contributing to antimicrobial resistance of P. aeruginosa against reactive chlorine species
2) Evaluate if these genes are also involved in host-pathogen interactions and immune invasion of P. aeruginosa biofilms
Methodological Approach: These studies will use analyse planktonic and biofilm cells of Pseudomonas aeruginosa. We will use a variety of different molecular and microbiological methods including functional genomics, fluorescence microscopy, gene cloning, antimicrobial killing assays, flow cytometry, gene expression analyses, host-pathogen interaction studies (e.g. gentamicin protection assay), etc.
Research area, student roles & skills
Research area: The increasing number of emerging multi-drug resistant human pathogens and the low number of antibiotics available to combat these infections is a significant threat to human health worldwide. Chronic infections frequently involve bacterial biofilms, which are especially difficult to treat due to the high antibiotic tolerance of biofilm cells and their extreme capacity for evading the host immune defence. Biofilms are microbial communities of cells embedded in a self-produced polymeric matrix composed of polysaccharides, proteins, lipids, and eDNA. Understanding antimicrobial resistance and mechanisms of host evasion of biofilms is crucial in the fight of antimicrobial resistant and persistent biofilm infections.
Student roles: The student will explore research literature to get familiar with the overall project. They will participate in laboratory and biosafety training. They will perform microbiological and molecular biological experiments with minimal guidance. The student will work with opportunistic human pathogens. After consultation with the supervisor, the student will be responsible for planing their weekly workload including planing of experiments and design of experimental methods. The student will participate in bi-weekly laboratory meetings as well as in regular research discussions. They will participate in lab-related duties such as maintenance of laboratory and equipment. They will provide a short project report and short research presentation (10min) at the end of the project.
Skills required: The student should have a background in biology, microbiology, health sciences, biochemistry, biomedical engineering, biomedical sciences, immunology or related fields and should be familiar with basic laboratory skills as well as basic microbiology methods including the culture of bacteria. Experience in advanced microbiology, microscopy, molecular biology (DNA, RNA, Protein), or genomics, would be an asset. The student should be a friendly, organized team player with interest in interdisciplinary research, who is highly self-motivated, and is able to work independently.
4. Assessing alternative splicing in placental development and dysfunction
This project will curate and analyze publicly available human placental RNA sequencing datasets to characterize alternative splicing patterns during placental development and dysfunction. The student will identify and download long-read or splice-aware RNA-seq datasets from public repositories such as those maintained by the National Center for Biotechnology Information and the European Bioinformatics Institute, prioritizing datasets with sufficient read length and coverage to assess alternative splicing events. After assembling a harmonized dataset and associated metadata, the student will use computational tools to quantify exon skipping, intron retention, and alternative splice site usage across gestation. Analyses will also examine differences in splicing patterns between fetal sexes and in pregnancies complicated by placental dysfunction, such as Preeclampsia or Fetal Growth Restriction. The goal of this project is to generate a curated resource and initial atlas of alternative splicing events in the human placenta, providing insight into transcriptomic regulatory mechanisms that shape placental development and may contribute to pregnancy complications.
Research area, student roles & skills
Research area: My research focuses on understanding the molecular mechanisms that regulate placental development and how disruptions to these processes contribute to pregnancy complications. My lab integrates genomics, epigenomics, and circulating nucleic acid analysis to characterize regulatory processes that shape placental cell differentiation and function. This includes studying DNA methylation, transcriptomic regulation, and circulating cell-free DNA signals that reflect placental biology. By combining molecular profiling with computational analysis, our work aims to identify regulatory pathways underlying normal placental development and dysfunction while advancing non-invasive approaches to study placental health during pregnancy.
Student roles: The student intern will play a central role in executing the project, working closely with the lab team to curate, organize, and analyze publicly available placental RNA-seq datasets. They will be responsible for identifying relevant datasets, downloading and managing sequencing files, and assembling associated metadata such as gestational age, fetal sex, and pregnancy outcomes. The intern will implement computational pipelines to detect and quantify alternative splicing events, compare splicing patterns across gestation, between sexes, and in pregnancies affected by placental dysfunction, and interpret these results in a biological context. They will also document methods, track analyses, and contribute to preparing figures and summaries for lab discussions and potential publications. This role offers hands-on experience in bioinformatics, transcriptomics, and integrative analysis of human developmental data while fostering independent problem-solving and scientific communication skills.
Skills required: Through this project, the undergraduate trainee will develop practical skills in computational genomics and transcriptomic data analysis. The student will gain experience working with large-scale sequencing datasets, including organizing data, extracting and harmonizing metadata, and applying bioinformatics tools to detect and quantify alternative splicing events. Training will include working in a Linux-based computing environment, using scripting and established analysis pipelines to explore transcript isoform variation across biological conditions. The trainee will also build skills in interpreting genomic data in a biological context, critically evaluating scientific literature, and communicating results through written reports and presentations. Together, these experiences will provide foundational
5. Assessing coral reef restoration success using biodiversity, benthic composition, and structural complexity metrics
Coral reef restoration is increasingly used worldwide to counteract reef degradation, yet the success of these interventions is rarely evaluated using standardized ecological metrics. In many cases, baseline data are lacking, making it difficult to assess the trajectory and effectiveness of restoration efforts. This project aims to evaluate coral reef condition and restoration outcomes using biodiversity, benthic composition, and structural complexity data from reef monitoring surveys in Indonesia. Depending on fieldwork progression, the student will either analyze pre-restoration baseline data or assess ecological patterns at already restored sites. The student will work with three main data types collected from standardized surveys: (1) fish diversity and abundance from transect counts, (2) benthic composition derived from photoquadrats processed using the ReefCloud platform, and (3) structural complexity metrics generated from 3D reef models using photogrammetry. The student will: (1) process and organize ecological datasets in R; (2) quantify fish diversity and community composition; (3) extract benthic cover metrics from annotated imagery; (4) assist in generating or analyzing 3D models to derive structural complexity metrics; and (5) integrate these components to assess patterns of reef condition and restoration success. This project will contribute to the development of standardized, multi-metric approaches for coral reef monitoring. The results will help identify key indicators of restoration success and inform future conservation strategies. The student will gain experience working with cutting-edge ecological tools, large datasets, and quantitative approaches in conservation science while being integrated into an active research group.
Research area, student roles & skills
Research area: My research focuses on biodiversity responses to environmental change, with an emphasis on linking community structure to ecosystem functioning and conservation outcomes. I use quantitative and trait-based approaches to understand how ecological communities respond to natural and anthropogenic disturbances across ecosystems. A key component of my work involves applying statistical and computational tools to large ecological datasets to assess biodiversity patterns, ecosystem services, and restoration outcomes. This research contributes to improving monitoring frameworks and informing conservation strategies in both freshwater and marine systems.
Student roles: The student will work closely with the supervisor and lab members to analyze coral reef monitoring data and assess indicators of ecosystem condition and restoration success. Their role will include: (1) organizing and cleaning ecological datasets derived from field surveys; (2) processing coral photoquadrat data using the ReefCloud platform to quantify benthic composition; (3) analyzing fish diversity and community structure from transect survey data; (4) supporting the generation or interpretation of 3D photogrammetry models to quantify reef structural complexity; and (5) integrating multiple ecological metrics to evaluate spatial patterns in reef condition. The student will apply statistical and multivariate analyses in R and will be guided through reproducible data workflows. They will also participate in weekly lab meetings, where they will discuss progress, receive feedback, and engage with broader topics in ecology and conservation. Training will be provided in ecological data handling, biodiversity analysis, and emerging monitoring technologies such as AI-assisted image classification and structure-from-motion photogrammetry. By the end of the internship, the student will have developed transferable skills in data analysis, visualization, and scientific interpretation, as well as experience working with real-world conservation datasets.
Skills required: The student should have a background in ecology, marine biology, environmental science, or a related field. Familiarity with concepts of biodiversity, community ecology, or conservation is expected. Experience with R programming and basic statistics is strongly preferred. An interest in data analysis and working with ecological datasets is essential. Previous experience with image analysis, GIS, or photogrammetry is an asset but not required. Motivation to learn new analytical tools and engage with interdisciplinary conservation research is important.
6. Beyond Obesity: The Effects of Diabetes on Fat and Muscle
Supervisor: Sylvia Santosa
University: Concordia University (Montréal campus)
Location: Montreal, Québec
Start date: 2027-07-26 (flexible)
Disciplines: Biological Sciences, Biology, Medicine, Medical Sciences, Nutrition, Molecular Biology, Pharmacology, Nursing, Physiology, Health Studies, Immunology
With obesity, results in fat that becomes dysfunctional. Dysfunctional fat contributes to the development of metabolic diseases such as diabetes and heart disease. Weight loss by bariatric surgery helps improve or cure metabolic disease. Since the majority of weight loss after bariatric surgery is from the fat located underneath our skin, it follows that the health of this fat depot may be important in disease remission. But what mechanisms does bariatric surgery change that causes disease remission? Losses of fat around the legs may be an especially key factor because when bariatric surgery patients do not lose much fat around their legs, the metabolic diseases they have does not improve. This study aims to determine how characteristics of leg fat compares to that of the stomach fat in people who: 1) are healthy and lean, 2) have obesity, and 3) have obesity and diabetes. We also want to know if unhealthy fat can become healthy after weight loss. We will compare the fat in the tummy and thighs for: 1) how fat cell behave and interact with muscle cells, 2) what is happening inside the fat cells, 3) the signals that they release. As 80% of fat lies underneath our skin, the results are important in understanding the mechanism by which fat increases metabolic disease risk.
Research area, student roles & skills
Research area: Our lab specializes in the study of nutrition, obesity and metabolism. We aim to answer the question of why some people with obesity develop diseases and others do not. To answer these questions, we examine the problem from a physiological and biological perspective by implementing a number of multidisciplinary techniques including nutrition and exercise interventions/measures, indirect calorimetry, flow cytometry, cell culture, PCR, etc. Our projects involve recruiting participants, conducting study visits, collecting and analysing samples (blood, adipose tissue, muscle). Interns will gain a multitude of skills and will be involved in some or all aspects of the study.
Student roles: The student will be assigned parts of the project based on their background, experience, and learning goals. Students may be involved in conducting the study, participants visits, collecting measurements, analysing samples, and/or analysing data.
Skills required: -Able to perform literature searches -Enthusiastic -Quick learner -Hard worker -Resourceful -Comfortable working with people -Lab experience, an asset -Previous experience with statistical analysis, an asset -French, an asset
7. Biochar a low-carbon solution for soil
Supervisor: Sumi Siddiqua
University: University of British Columbia (Okanagan campus)
Landfills remain the predominant method for the secure disposal of municipal and hazardous wastes worldwide. Although modern landfills are generally engineered to high standards, the basal lining systems may still fail to fully prevent leachate migration and the infiltration of harmful substances into groundwater. Such failures can lead to the release of hazardous contaminants into adjacent water bodies and coastal environments. The research primarily examines the unsaturated behaviour of soils amended with biochar and BHET polymer, focusing on shrinkage, electrical conductivity, and water retention performance of the composite liner material. A series of compressive strength, volumetric shrinkage, water retention, and electrical conductivity tests was conducted, and scanning electron microscopy (SEM) was employed to assess microstructural features. This study underscores the potential for repurposing PET waste in hydraulic barrier construction due to its non-toxic nature, cementitious behaviour, heavy metal adsorption capacity, ready availability, and low biodegradability.
Research area, student roles & skills
Research area: My research activities focus on: (i) binder technology for road and construction materials; (ii) carbon capture strategies applied to binders; (iii) adsorption studies involving novel regenerative hybrid magnetic SBA-15 materials; (iv) the characterization and remediation of contaminated soils; (v) the influence of temperature and pore fluid salinity on clay barriers; (vi) the mechanical behavior of clay barriers under varying suction levels; and (vii) the smart design of permeable reactive barriers for microplastics pollution control. My work is primarily experimental, and I have established a strong, hands-on training program in my laboratory to support the development of highly qualified personnel (HQP).
Student roles: The research laboratory is equipped with advanced testing facilities that require specialized training for safe and effective operation. The selected student will receive comprehensive training on the use of laboratory equipment, along with the necessary theoretical background to conduct research in accordance with established protocols developed by previous researchers. The student will work in a collaborative environment alongside a team of scholars, exchanging ideas, sharing resources, and supporting peers as needed. Strong time management skills are essential, as the position involves meeting defined milestones and maintaining regular interactions with industrial partners. The student will be expected to adhere to clear deadlines for completing assigned deliverables. Key responsibilities will include collecting and analyzing experimental data, conducting statistical analyses, and using relevant computational tools and software in the research domain. While the overall objectives of the project will be clearly outlined, the student will be encouraged to contribute to the development and refinement of experimental protocols. This approach fosters critical thinking, independence, and innovation. Additionally, it promotes effective teamwork by enabling the student to leverage their individual strengths while contributing to a cohesive, productive research environment.
Skills required: The ideal intern should demonstrate perseverance and strong enthusiasm for research, enabling them to effectively engage with experimental challenges. Excellent communication and time management skills are essential to balance research tasks with academic commitments. The student must be capable of working both independently and collaboratively, showing initiative and contributing positively to team efforts. Strong problem-solving and multitasking abilities are required, along with a willingness to learn new technologies and techniques. Regular participation in weekly research meetings is expected. Additionally, the intern must uphold strict confidentiality and maintain professional and ethical standards in handling all research data and project outcomes.
8. Biomarkers of Mitochondrial Stress and Metabolic Dysfunction to Predict Treatment Outcomes in Mood Disorders
Supervisor: Ana Silva
University: University of Saskatchewan (Saskatoon campus)
Mood disorders such as major depressive disorder (MDD) and bipolar disorder (BD) remain difficult to diagnose and treat due to their biological heterogeneity. Increasing evidence from clinical, genetic, and neuroimaging studies implicates mitochondrial dysfunction in the pathophysiology of these disorders, including alterations in mitochondrial DNA (mtDNA) damage, pathogenic mtDNA variants, inflammatory signaling and energy metabolism. However, mitochondrial dysfunction is typically assessed using individual biomarkers that do not capture the complexity of mitochondrial health in mood disorders.
To address this, we will develop and validate a peripheral Mitochondrial Integrity Index (pMII), a multifactorial algorithm that integrates blood‑derived mitochondrial, metabolic and cellular stress markers into a composite measure of mitochondrial health. Unlike single mitochondrial biomarkers, the pMII integrates multiple dimensions of mitochondrial biology into a single quantitative index. The pMII will be evaluated for its ability to inform prognosis and predict treatment outcomes in mood disorders. To achieve this overarching goal, we have designed the main objective described below for a translational pipeline linking mitochondrial biology to biomarker-guided diagnostics in mood disorders. Sex‑ and gender‑related effects will be evaluated.
Objective 1 - Develop a Peripheral Mitochondrial Integrity Index (pMII) for mood disorders.
a) Characterize the pMII across mood disorders and healthy controls.
b) assess associations between pMII and key clinical features, including metabolic markers, symptom burden and treatment outcomes.
Impact: This program addresses a critical unmet need for biomarker-driven diagnosis and treatment stratification in mood disorders. By bridging mechanistic and translational approaches, this research will advance understanding of mitochondrial and metabolic biology in psychiatry while accelerating the development of practical diagnostic tools.
Research area, student roles & skills
Research area: My research focuses on understanding how mitochondrial dysfunction, oxidative stress and inflammation disrupt brain homeostasis and function, contributing to psychiatric disorders such as Major Depression and Bipolar Disorders. Leveraging complementary human samples (e.g., blood), patient-derived neuronal and glial models, and in vivo systems (drosophila and mouse models), we evaluate mitochondrial fitness, bioenergetic balance, and neuroinflammatory signaling. Our work integrates genome editing and bioengineering approaches to interrogate pathogenic mitochondrial DNA (mtDNA) variants and damage, mitokines, and inflammatory biosignatures as convergent indicators of cellular metabolic stress and disease vulnerability.
Student roles: Weeks 1–4: Training and Pilot Work Complete mandatory safety and ethics training (laboratory biosafety, whims and others). Receive training in participant recruitment, informed consent procedures, and ethical conduct of human research. Learn how to administer and score standardized self-report and clinician-administered questionnaires, including assessments of depressive symptoms, affective temperament, and metabolic health. Participate in participant interviews and clinical assessments under supervision to gain experience in psychiatric research procedures. Receive hands-on training in blood sample processing, plasma and buffy coat isolation, DNA extraction, mitochondrial isolation, and qPCR.
Weeks 5–8: Experimental Phase Process participant blood samples and perform plasma and buffy coat isolation. Extract circulating DNA from plasma samples and quantify DNA concentration and purity. Quantify mitochondrial DNA levels and deletion rates using real-time qPCR. Perform mitochondrial bioenergetic assays, including electron transport chain (ETC) Complex I–IV activity measurements. Measure ATP levels as an indicator of mitochondrial function. Quantify circulating mitochondrial stress markers using ELISA-based assay.
Weeks 9–12: Data Analysis and Presentation Organize and analyze clinical, metabolic, and mitochondrial datasets. Assist in constructing the Peripheral Mitochondrial Integrity Index (pMII) by integrating mitochondrial bioenergetic, molecular, and metabolic measures. Explore associations between pMII scores and clinical outcomes, including symptom severity and metabolic characteristics. Prepare figures and report findings in a short manuscript. Present results at a lab meeting or student symposium.
My training program will focus on nurturing essential academic skills, including theoretical knowledge acquisition, laboratory techniques, data analysis, and scientific communication through team meetings and journal clubs. I will foster a welcoming environment that meets needs of both domestic and international students and organize social events to promote team bonding and collaboration. To ensure a smooth transition for the international student, I will actively engage with the International Student and Study Abroad
Skills required: The student should have a background in biological sciences, neuroscience, molecular biology, genetics, pharmacology, or related fields, with solid knowledge of molecular and cellular biology. Basic lab skills such as pipetting and solution preparation are required. Experience with techniques like DNA extraction, PCR, histology, westernblot or ELISA. Familiarity with data analysis tools (e.g., SPSS, ImageJ) is also beneficial. Strong communication skills, both written and verbal, are essential for teamwork and presentations. The student must be detail-oriented, able to work independently and collaboratively, and committed to following protocols and safety guidelines.
9. Building AI-ready biodiversity datasets from camera and acoustic monitoring in the Amazon
Monitoring biodiversity in tropical ecosystems remains a major challenge due to the high diversity of species and the logistical difficulty of field surveys. Recent advances in automated sensors—including camera traps and acoustic recorders—are generating large volumes of ecological data, but transforming these data into usable information requires extensive annotation and preparation. This project focuses on supporting biodiversity monitoring efforts in the Amazon, particularly within Kayapó Indigenous territories, using camera and acoustic recordings from automated sensor networks. These systems collect visual and sound data on multiple taxonomic groups, including mammals, birds, bats, and fishes, creating large multimedia datasets. The student will contribute to preparing these datasets for artificial intelligence applications. Specifically, they will: (1) annotate images and audio recordings to identify organisms or key signals; (2) organize and curate datasets for training and validating machine learning models; (3) assist in cleaning and structuring metadata associated with recordings; and (4) explore patterns in biodiversity data across sites and taxonomic groups. While the focus is on data preparation rather than model development, the student will gain exposure to AI workflows and biodiversity informatics. This work is critical for enabling automated species recognition systems and improving large-scale biodiversity monitoring. The project contributes to building open, high-quality datasets for conservation science and supports ongoing efforts to develop AI tools for monitoring Amazonian biodiversity at scale. The student will gain experience at the intersection of ecology, data science, and conservation technology.
Research area, student roles & skills
Research area: My research focuses on biodiversity monitoring and ecological data analysis, with an emphasis on integrating emerging technologies such as artificial intelligence into ecological research. I study how ecological communities can be monitored and understood using large, complex datasets derived from sensors, imagery, and acoustic recordings. This work aims to improve biodiversity assessment and conservation outcomes by combining ecological theory with computational tools, contributing to scalable and automated monitoring systems in data-limited regions.
Student roles: The student will work closely with the supervisor and collaborators to prepare biodiversity datasets derived from camera traps and acoustic monitoring systems in the Amazon. Their role will include: (1) annotating images and audio recordings to identify species or relevant biological signals; (2) organizing and curating large multimedia datasets for use in machine learning workflows; (3) cleaning and structuring associated metadata, including spatial and temporal information; and (4) assisting in exploratory analyses to summarize biodiversity patterns. The student will gain hands-on experience working with real-world ecological datasets generated by automated monitoring technologies. They will be introduced to best practices in data management, reproducible workflows, and dataset preparation for AI applications. The student will also participate in weekly lab meetings and discussions on ecology, conservation, and emerging technologies. Training will be provided in ecological data handling, annotation tools, and basic principles of machine learning as applied to biodiversity monitoring. By the end of the internship, the student will have developed transferable skills in data curation, biodiversity informatics, and interdisciplinary research linking ecology and artificial intelligence. They will also contribute to datasets that support the development of automated biodiversity monitoring tools.
Skills required: The student should have a background in ecology, biology, environmental science, computer science, or a related field. Interest in biodiversity and conservation is essential. Experience with data handling in R or Python is strongly preferred. Familiarity with image or audio data processing is an asset but not required. Attention to detail and ability to work with large datasets are important. Interest in artificial intelligence applications in ecology is highly desirable.
10. Building Freshwater Resilience in Climate-Stressed Boreal Watersheds Through Integrated Ecosystem Monitoring
Supervisor: Felix Nwaishi
University: Mount Royal University (Calgary campus)
This research project aims to understand how climate change and human disturbances are affecting freshwater systems across Canada's boreal region and to identify solutions that strengthen long-term freshwater resilience. Boreal watersheds contain globally important freshwater reserves, wetlands, and peatlands that regulate water quality, support biodiversity, store carbon, and sustain Indigenous communities. However, these ecosystems are increasingly threatened by cumulative disturbances such as wildfire, drought, permafrost degradation, forestry, roads, and energy development.
The project will establish an integrated freshwater monitoring network across multiple peatland-dominated watersheds in northern Alberta. Through field-based ecosystem monitoring, the study will assess how environmental disturbances influence hydrological connectivity, water quality, aquatic biodiversity, and ecosystem resilience. Monitoring activities will include measurements of water levels, streamflow, water chemistry, dissolved organic matter, nutrients, trace metals, and biological indicators of ecosystem health. Environmental DNA (eDNA) and biodiversity assessments will be used to evaluate ecological responses to environmental change.
A key component of the project is the integration of Indigenous knowledge and western ecosystem science through a community-informed monitoring framework. Indigenous partners and community members will help identify monitoring priorities, culturally important freshwater indicators, and stewardship opportunities. The project will also apply machine learning and advanced data analytics to identify cumulative environmental impacts, develop early-warning indicators of freshwater degradation, and predict watershed vulnerability under future climate scenarios.
The expected outcomes include new scientific knowledge on freshwater resilience, improved watershed monitoring approaches, predictive tools for climate adaptation, and guidance for ecological restoration and nature-based solutions. The project will contribute to freshwater conservation, Indigenous stewardship, and evidence-based environmental management while providing undergraduate researchers with opportunities to gain hands-on experience in field research, laboratory analysis, environmental monitoring, data science, and interdisciplinary ecosystem science.
Research area, student roles & skills
Research area: My research specializes in ecosystem science, with a focus on understanding how climate change and human disturbances affect freshwater ecosystems, wetlands, and boreal landscapes. I investigate the cumulative impacts of wildfire, drought, permafrost thaw, resource development, and urbanization on water quality, biodiversity, and ecosystem resilience. My work integrates ecohydrology, environmental chemistry, microbial ecology, and advanced monitoring technologies, including environmental DNA (eDNA), remote sensing, artificial intelligence, and machine learning. Through collaborations with Indigenous communities, government agencies, and industry partners, I develop science-based solutions that support ecological restoration, climate adaptation, freshwater stewardship, and sustainable environmental management.
Student roles: The undergraduate student will play an active role in both the field and laboratory components of the project. Responsibilities will include assisting with freshwater ecosystem monitoring, water and sediment sample collection, environmental DNA (eDNA) sampling, biodiversity surveys, and hydrological measurements across boreal watershed sites. Students will support the deployment and maintenance of monitoring equipment, including water-level loggers, water-quality sensors, and meteorological instruments. In the laboratory, the student will assist with sample processing, water quality analyses, data entry, quality assurance/quality control procedures, and the organization of environmental datasets. The student will also contribute to data analysis, literature reviews, and the interpretation of research findings using statistical and geospatial tools. Opportunities will be provided to participate in machine learning applications for environmental monitoring and freshwater resilience assessment. The student will work closely with researchers, community partners, and Indigenous collaborators, gaining exposure to interdisciplinary and community-engaged research approaches. They will participate in research meetings, contribute to project reporting, and assist in the preparation of presentations, posters, and scientific publications. Through these activities, the student will gain hands-on training in freshwater science, ecosystem monitoring, environmental data analysis, and scientific communication while contributing to research that supports climate adaptation, freshwater stewardship, and ecosystem resilience.
Skills required: This project is suitable for undergraduate students in environmental science, ecology, biology, geography, hydrology, environmental chemistry, data science, or related disciplines. Students should have a strong interest in freshwater ecosystems, climate change, environmental monitoring, and sustainability. Experience in fieldwork, laboratory analysis, GIS, statistics, or programming (R or Python) is an asset but not required. Students should be comfortable working both independently and within interdisciplinary teams. Training will be provided in water quality monitoring, biodiversity assessment, environmental DNA (eDNA), data analysis, and scientific communication, offering valuable preparation for graduate studies and environmental careers.
11. Cannabinoids effects on sleep and breathing
Supervisor: Silvia Pagliardini
University: University of Alberta (Edmonton campus)
Location: Edmonton, Alberta
Start date: 2027-05-03 (flexible)
Disciplines: Biological Sciences, Biology, Medical Sciences, Medicine, Neuroscience, Molecular Biology, Pharmacology, Physiology, Veterinary Science and Medicine
Although a multitude of undocumented evidence suggests that marijuana (cannabis) promotes relaxation and sleep, there is a lack of systematic scientific studies showing this to be the case. Medically, cannabis may provide fundamental health benefits if it can promote the full spectrum of sleep and also if it has beneficial effects on breathing during sleep (unlike opioids). The purpose of this project is to systematically explore the effects of cannabis principal components on sleep dynamics and breathing in rodents in order to evaluate its potential therapeutic benefits and/or harms. In this way, we can explore how acute and chronic cannabis (or its components) use may impact both sleep and respiratory functions through development and in adulthood.
We will use animal models of both sexes to strengthen the evidence base for this targeted approach and build pre-clinical scientific and health related knowledge for future development of a much needed treatment for both sleep and sleep disordered breathing.
Research area, student roles & skills
Research area: Research conducted in my laboratory aim to gain an understanding of the neuronal mechanisms that control breathing and affect its function during sleep when the majority of respiratory disorders of central origin occur. We are currently interested in delineating the function of a region in the brainstem that is crucial for the generation of expiratory activity. Its contribution to ventilation in health and disease is yet not clear. With state of the art technologies we aim to provide a better understanding of the function of this structure and the networks that influence its activity.
Student roles: The student will be responsible for the surgical procedures, acquisition of data, data analysis, and elaboration of the results. The student will acquire expertise in survival surgeries, stereotaxic injection, animal handling, EEG/EMG instrumentation, and optogenetic techniques. She/He will also gain experience on data analysis of EEG and respiratory variables in anesthetized rodents.
Skills required: background in neuroscience, neuro-physiology, pharmacology and respiratory physiology
12. Cannabinoids for pain and inflammation
Supervisor: Christian Lehmann
University: Dalhousie University (Halifax campus)
Location: Halifax, Nova Scotia
Start date: 2027-05-03 (flexible)
Disciplines: Biological Sciences, Immunology, Medical Sciences, Medicine, Microbiology, Molecular Biology, Pharmacology, Biology, Neuroscience, Veterinary Science and Medicine
Inflammation is associated with the release of pro-inflammatory cytokines such as TNF-α, IL-1, IL-6. These cytokines play a role in cellular apoptosis and tissue damage. The endocannabinoid system has been shown to play a role in both acute and chronic inflammation. Cannabinoid type 2 receptors (CB2R) are expressed on immune cells and activation of CB2R attenuate immune cell (hyper-)activation. Therefore, treatment with CB2R agonists or inhibition of CB2R agonist degradation represents a promising anti-inflammatory approach.
In this project we assess the effects of CB2R activation on local and systemic inflammation and infection in whole animal models. Specifically, we will be investigating the effects of natural CB2R agonists on leukocyte adhesion, tissue damage and inflammatory cytokine levels in experimental cystitis. Given the role CB2R plays in inflammation and leukocyte recruitment, we believe that by further activation of CB2R we can decrease tissue damage and leukocyte recruitment in states of inflammation.
Research area, student roles & skills
Research area: Dr. Lehmann’s clinical training in the related disciplines of anesthesia, intensive care medicine, pain therapy and emergency medicine factor heavily in his main research focus on local and systemic inflammation. Through his experimental research, Dr. Lehmann seeks to identify novel treatments for pain and inflammation. In preclinical studies, his team utilizes intravital microscopy to visualize activation of immune cells in vivo. Modulation of the endocannabinoid system represents an innovative approach to attenuate inflammation and achieve pain relief in several clinical conditions, such as cystitis, inflammatory bowel diseases or sepsis.
Student roles: The student will be directly involved in the research project and will be assisting a post-doctoral fellow. The student will help with all experimental procedures and data analysis. By the end of the internship the student will have had an opportunity to practice animal handling (mice), intravital microscopy, tail-vein injections, data analysis (leukocyte recruitment data), histology and other general laboratory skills such as solution preparation. The student will be responsible for showing up on time and assisting with the experiment set up, the experiment and the cleanup. The student will be expected to become comfortable with conducting a literature review and to learn the background and current information about the research project. Additionally, during the internship the student will be expected to attend lab meetings and to participate in journal club.
Skills required: The student is required to have a basic understanding of the biological sciences with a specific emphasis on microbiology and immunology. Previous laboratory experience with animal work (specifically mice), intravital microscopy, tail-vein injections, data analysis (leukocyte recruitment data), histology and other general laboratory skills such as solution preparation and handling are an asset but are not required. Most of the skills required will be taught during the internship.
13. Cardiovascular health in response to exercise training in postmenopausal women
Supervisor: Jennifer Williams
University: Wilfrid Laurier University (Waterloo campus)
Aging-related declines in cardiometabolic health, including reductions in brain, heart, and arterial function, and impaired glucose regulation, are accelerated after menopause, in part due to shifts in sex hormone levels. Although exercise training is known to improve cardiometabolic health, women remain underrepresented in this research, particularly after the menopause transition. Notably, very few studies have examined how exercise training affects cardiometabolic outcomes in postmenopausal women. The purpose of this pilot study is to evaluate the effects of a 12-week exercise training program (EX) compared with a physical activity counselling-only intervention (CON) on cardiometabolic health outcomes in postmenopausal women. We hypothesize that both interventions will offer improvements to cardiometabolic health, with greater improvements in the EX group. We will recruit 28 postmenopausal women within 2-8 years of their final menstrual period. Participants will undergo baseline assessments and then be randomized to either a 12-week aerobic and resistance training program aligned with Canadian physical activity guidelines or to a counselling-only condition. The EX group will complete supervised sessions twice weekly at the Centre for Healthy Communities. Pre- and post-intervention assessments will include cardiac and vascular measures (endothelial function, arterial stiffness, carotid intima-media thickness, left ventricular ejection fraction), cerebrovascular function, and insulin resistance via an oral glucose tolerance test. Participants will also complete fitness testing, strength assessments, and lifestyle behaviour measures (physical activity, sleep, diet). This study will be the first to comprehensively assess guideline-based exercise training on cardiometabolic, vascular, and brain health outcomes in postmenopausal women, providing essential data to inform future research in this understudied population. The student intern on this project will be involved in all stages of the research project, including recruitment and screening, data collection, intervention administration, analysis of outcomes, and knowledge diseemination efforts, as part of a larger lab team.
Research area, student roles & skills
Research area: My research program focuses on how the cardiovascular system is impacted by metabolic, mechanical, and psychological “challenges” with a focus on sex-differences and female-specific physiology. We study sex-differences in cardiovascular outcomes in young, middle-aged, and older males and females. We also look at how the cardiovascular system changes during periods of sex hormone reduction (natural and surgical menopause, breast cancer survivors using aromatase inhibitors) or with sex hormone addition/fluctuations (menstrual cycle, oral contraceptive use). With my background in exercise physiology, I am also interested in how different exercise training programs impact cardiovascular health, with a focus on women.
Student roles: The student will be involved in all aspects of the project including recruitment and screening of participants, participating in data collection and intervention administration, analysis of data, and preparing knowledge dissemination materials. They will also be a part of a larger research lab group of 12 trainees and staff, so be able to take part in other projects throughout the summer.
Skills required: We are looking for engaged and passionate students who have an academic background in the Life Sciences, Biological Sciences, or Kinesiology, or similar disciplinary areas. Required skills include the ability to work both independently and as part of a larger group setting, experience with engaging with members of the community preferably in a healthcare setting or similar environment. Assets include experience with exercise training, cardiovascular measurements, and working in research environments. We welcome individuals to apply from diverse backgrounds, and especially welcome women+, 2SLGBTQIA+, and BIPOC students.
14. Cartography of Candida s response to antifungals
Supervisor: Claire Kamaliddin
University: University of Alberta (Edmonton campus)
Cartography of Candida s response to antifungals in co culture models: this project aims to caracterize fungal s response to drugs in realistic media. The student will set up co culture models of the yeast candida with human cells, and compare antifungal susceptibility in different environments and conditions, from a phenotypic, and molecular standpoint.
Research area, student roles & skills
Research area: I am a Pharmacist Scientist leading a new research program at the Faculty of Pharmacy at the University of Alberta. My team, POINT (pathogens omics interaction teams) uses multi omics methods to study host pathogen interaction in the context of antimicrobial resistance and persistent infections. We use a blend of clinical and laboratory studies, and our work is focused on parasitic and fungal infections.
Student roles: The student will conduct wet-lab experiments using laboratory strains of pathogens and human cell line. The internship will blend lab work (for the first 6 weeks, including co-culturing pathogens and human cell lines), and omics data analysis (for the second part of the internship). The contingency plan includes access to other data generated in the lab for the second part of the internship, to ensure a complete learning experience.
Skills required: The student must have a background in biomedical sciences, pharmacy, or medicine. Previous lab or data analysis experience is preferred, but not required. Specific skills include molecular biology experience (nucleic acid and protein purification, PCR), cell culture, and omics data analysis. R software user.
15. Cell and molecular biology of insect cold tolerance
Supervisor: Jantina Toxopeus
University: St. Francis Xavier University (Antigonish campus)
Overwintering insects have many remarkable adaptations that we can study at the cellular and molecular level. The Toxopeus lab uses two model organisms to address questions about these adaptations: the freeze-tolerant spring field cricket (Gryllus veletis) and the freeze-avoidant eastern spruce budworm (Choristoneura fumiferana) - a dominant insect pest of the boreal forests in Canada. We use the crickets as a model to study how freeze-tolerant insects modify their biology in order to survive ice formation inside their bodies. We use spruce budworm as a model to understand how overwintering insects remain dormant and stress-tolerant, preventing ice formation to temperatures lower than -30C! To study both of these questions, the Toxopeus lab uses standard molecular and cell biology techniques.
In freeze-tolerant crickets, we use RNA interference to knock down genes hypothesized to play a role in freeze tolerance. An important part of these experiments is validating that the knockdown worked by measuring the expression of the target gene in the freeze-tolerant crickets. This is done at both the RNA level (e.g., with quantitative PCR) and the protein level (e.g., with Western blotting or immunocytochemistry), and then followed by testing whether the knockdown affects the crickets' ability to survive freezing.
In spruce budworm, we have used transcriptomics to identify key genes that may regulate dormancy and stress tolerance. An important next step is testing how these genes change their expression over time during dormancy (e.g., via quantitative PCR), along with their gene products (proteins or metabolites), and whether we can use specific gene expression patterns to predict when dormancy will end.
This project will involve measuring gene expression or its projects in either the freeze-tolerant crickets or overwintering spruce budworm, depending on intern interests.
Research area, student roles & skills
Research area: The Toxopeus lab (https://jantinatoxopeus.com/) focuses on understanding how animals and their cells survive in challenging environments, especially low temperatures associated with winter. This is important for understanding how organisms tolerate current environmental stressors, and how they might respond to future changes in their environment (e.g. due to global climate change), with implications for forestry, agriculture, and human health (e.g., when studying insect pests and vectors of diseases). Our work on freeze-tolerant insects also has implications for understanding cryopreservation, an important technique for preserving cells and tissues for biomedical purposes.
Student roles: The student will learn how to dissect insect tissues, use techniques involved in studying gene expression (e.g., RNA extraction, quantitative PCR, protein extraction, Western blotting, immunocytochemistry, confocal microscopy), and record the data from these experiments in an organized fashion. The student will also learn how to analyze the data and present it in visual or graphical form to share the information with their supervisor and other scientists. The student will also be expected to spend some time learning about the project by reading scientific literature, and may be expected to contribute to the process of publishing the results from their project. All students in the Toxopeus lab regularly meet with Dr. Toxopeus and participate in group laboratory meetings.
Skills required: Some background in molecular biology is an asset, but not required. Some experience using the techniques described in the “required role” section would also be an asset, but are not required. Students will be fully trained in relevant background knowledge and techniques for their project while in the Toxopeus lab.
Generally, research projects are most successful when students have strong attention to detail, organization, and time-management skills. It is also important to be able to work well independently and as part of a team. Finally, a general curiosity about the way biology works is always good!
16. Cellular Concentrations and Cytotoxicity of Lithocholic Acid and Its Sulfonated Metabolite
Lithocholic acid (LCA), a toxic secondary bile acid, induces DNA damage, hepatotoxicity, and promotes carcinogenesis. It is conjugated to glycine to form glycolithocholic acid (G-LCA) or to taurine to form taurolithocholic acid (T-LCA). They are sulfonated to lithocholic acid sulfate (LCA-S), glycolithocholic acid sulfate (G-LCA-S), taurolithocholic acid sulfate (T-LCA-S) by sulfotransferase 2A1 (SULT2A1) to enhance their solubility for excretion. SULT2A1, a Phase II drug metabolizing enzyme, is also responsible for the metabolism of many other drugs (e.g., abiraterone) and endogenous chemicals (e.g., dehydroepiandrosterone).
Steroidal anti-androgen drugs are used for the treatment of advanced or metastatic prostate cancer. Previously, we reported that steroidal anti-androgen drugs, galeterone and abiraterone, are potent inhibitors of dehydroepiandrosterone sulfonation catalyzed by human liver cytosol, SULT2A1, SULT2B1b, and SULT1E1 (Yip et al., Drug Metab Dispos 2018, 46: 470-482). We also found that these drugs inhibit SULT2A1-catalyzed sulfonation of LCA, G-LCA, and T-LCA. However, it is not known whether there are differences in LCA and LCA-S transport into the cells, and whether LCA or LCA-S is responsible for the cellular toxicity of LCA?
Objectives:
The aim of the study is to determine the relative intra- and extracellular concentrations of LCA and LCA-S in HepG2 human hepatocellular cells treated with LCA and LCA-S, and to determine their cytotoxicity.
Methods:
The experiments will be conducted in cell culture models. Cells will be treated with various concentrations of LCA or LCA-S for various durations. The culture media and the lysates will be harvested. Total protein concentration will be measured. The concentration of LCA and LCA-S will be determined using highly quantitative ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). Cytotoxicity will be determined using lactate dehydrogenase (LDH) assay.
Significance and Implications:
The findings will provide mechanistic explanations to our findings that inhibition of LCA-S formation increases LCA toxicity to cells.
Research area, student roles & skills
Research area: My current research program is in the areas of drug metabolism, pharmacokinetics, functional pharmacogenetics/epigenetics, and toxicology. The overarching theme of my research program is to enhance our understanding of the mechanistic basis of individual variation in pharmacokinetics and pharmacodynamics, thereby contributing to personalized pharmacotherapy by improving drug effectiveness and safety in the individual patient. Specifically, the aim of my research program is to elucidate the various genetic and non-genetic factors (e.g., drugs/toxicants/endogenous chemicals, food, epigenetics) involved in regulating drug metabolism, transport, and action.
Student roles: The student will be involved in literature search/reading, planning of experiments, preparation of reagents (including calculations of reagents/chemicals and concentrations required for each step of the experiments), learning and performing experiments in the laboratory, writing of laboratory reports/notes, data analysis using Excel formulas, and statistical analysis using SigmaPlot software. As a member of the laboratory team, the student will also play a role in laboratory management tasks. The student may also be involved in presenting experimental findings in laboratory meetings and/or research symposium (if opportunity exists). The student will be trained to work independently on experiments. Some of the experimental techniques include cell culture/subculture, preparation of chemicals, in situ incubation of the cells with chemicals, cell lysis, performing total protein quantification assays, preparing standard calibration curves, and preparing samples for UHPLC-MS/MS analysis.
Skills required: The project requires highly quantitative experimental techniques to obtain accurate and precise data. The quantitative project is particularly suitable for students who are highly meticulous, highly organized, and with a very strong interest in pharmacology, drug metabolism, pharmacokinetics, or biochemistry. Students should have a background in pharmacy, pharmacology, biochemistry, biomedical sciences, biological sciences, or other related fields. Students with pharmaceutical analysis/bioanalytical chemistry, and cell culture skills and knowledge will have an advantage in performing the experiments in this project.
17. Chromatin dynamics during Equine herpesvirus infection
Supervisor: Kristen Conn
University: University of Saskatchewan (Saskatoon campus)
Equine herpesvirus (EHV1) is common throughout horse populations worldwide. EHV1 establishes a life-long infection that is largely asymptomatic. However, symptomatic disease manifestations can be severe and include neurological or respiratory disease, abortion, and even neonatal death. More knowledge of the molecular mechanisms of EHV1 infection is necessary to inform the development of new strategies to treat or prevent EHV1-associated diseases. We will use knowledge of the closely related alphaherpesvirus, herpes simplex virus 1 (HSV1), to guide our characterization of the molecular mechanisms that regulate EHV1 chromatin and investigate how chromatin regulates EHV1 infection.
Lytic HSV1 genomes are assembled into highly dynamic, transcriptionally competent, chromatin. Consistent with the highly dynamic HSV1 chromatin, HSV1 transcription activators destabilize chromatin by increasing the chromatin exchange of histones (histone dynamics). We discovered that EHV1 also increases the chromatin exchange of histones, although whether this is achieved by EHV1 transcription activators is unknown. We aim to evaluate whether the EHV1 transcription activator, IE1, or transcription repressor, IR2, alter histone dynamics.
Research area, student roles & skills
Research area: We study the interactions between viruses and host cells. Specifically, we investigate how viruses attempt to take over the cell nucleus and how the cell attempts to prevent this takeover. We use herpes simplex virus (HSV1) and related veterinary alphaherpesviruses to investigate the roles of chromatin in this "battle for the nucleus". We have identified a novel chromatin regulatory function for the only essential HSV1 transcription activator, ICP4. We aim to characterize how ICP4 regulates viral chromatin to activate viral gene expression and evaluate whether this activity is conserved among the ICP4 homologues of veterinary alphaherpesviruses.
Student roles: The student will be directed and trained in all techniques and background knowledge required to conduct their research project. They will independently engage in their project with oversight, supervision, and mentorship from the PI and laboratory technician. The student will meet with the PI weekly to review their progress, evaluate results, address any arising issues, and plan the research activities for the next week. They will also participate and present in weekly laboratory group meetings and events hosted by the Department of Veterinary Microbiology. The student will present their research project and results at the annual Western College of Veterinary Medicine Undergraduate Research Poster Day. We have cloned the sequences encoding IE1 and IR2 from EHV1 genomic DNA into mammalian expression vectors as RFP (red fluorescent protein) fusion proteins. The student will use these constructs to create stable cell lines with inducible expression of RFP-IE1 or -IR2 fusion proteins (or RFP as a control). The student will use these cells to evaluate histone dynamics in the presence of RFP-IE1, RFP-IR2, and RFP to test whether either viral protein alters histone dynamics. A live cell confocal imaging technique called FRAP (fluorescence recovery after photobleaching) is used to directly measure the mobility of GFP (green fluorescent protein)-histone fusion proteins. We already have plasmids encoding GFP-histone fusion proteins. The student will prepare lentiviral stocks for transduction of equine cells (to make the stable cell lines) and evaluate and optimize RFP-IE1, -IR2, or RFP expression. The student will prepare stocks of GFP-histone expression plasmids and maintain the stable cell lines for their experiments. The student will transfect cells with individual GFP-histone-encoding plasmids, induce expression of the protein of interest, and evaluate histone mobility by FRAP.
Skills required: The student should have basic knowledge in the areas of cell biology and molecular biology with some experience in common molecular biology techniques, including micropipetting. Practical experience with tissue culture techniques would be an asset, but is not required. The student should have excellent oral and written English communication abilities and possess strong problem-solving, organization, and interpersonal skills. The student should have a positive attitude, willingness to self-asses and improve, and take initiative in their learning and research activities.
18. Climate impacts on the ecology of a water-limited forest in western Canada
Tree growth and mortality rates have a strong bearing on the structure, biomass, and carbon balance of forests. Potential decreases in growth or increases in mortality from drought and insect pests may threaten the long-term persistence of forest cover near the prairie-forest ecotone in western Canada, leading to the loss of ecosystem services provided by forests in this region. This research project will collect aerial data to better understand how tree demography is related to moisture availability in the interior of western Canada. We will use an Uncrewed Aerial Vehicle (UAV) to carry out 3D surveys of forest canopies across the Cypress Hills region of Saskatchewan and Alberta, and use the resulting data to elucidate relationships between water availability, growth, survival, and biomass for several tree species. These data will help us to quantify how forest structure is related to tree demography, competition, and local environmental conditions. Results from this project will help in understanding how future climate change may affect forests in Canada's western interior over the coming decades.
Research area, student roles & skills
Research area: My lab's research seeks to better understand how forests will be affected by global change. We are particularly interested in how tree-level demographic processes interact with disturbance to create changing patterns of structure, biomass, and diversity within forest stands and landscapes.
Student roles: The internship will include a combination of field-based and computer-based tasks performed under the supervision of a graduate student or postdoctoral fellow. In the field, the student will assist in performing UAV surveys in remote forest sites. The research team will spend several weeks working from the university's field station while they perform data collection. Once this work is completed the team will return to the university, where the student will process imagery data collected from the UAV surveys using GIS software and prepare it for statistical analysis. While on campus the student will have the opportunity to participate in networking events and professional development activities with other research students.
Skills required: The student should have a background in biology, forestry, environmental science, geography, or a related field. They should be physically fit and enjoy working outdoors in a remote forest environment. They should be hard-working in collecting field data and in an efficient and reliable manner. They should have some experience in analyzing data using computer software, and be conscientious in ensuring the quality and integrity of data processing workflows. Previous experience using geographic information systems (GIS) software would be valuable, but is not required.
19. Comparing and benchmarking genomic imputation algorithms in a non-model system
Genotype imputation has gained traction as a way of inferring the genotypes of individuals that have only been sequenced to low depth (e.g., 1X). Essentially, these approaches use Hidden Markov Models (HMMs) to infer the most likely haplotype from given reference haplotype set (i.e., the "reference panel") to which the low-coverage target sample likely belongs, thereby allowing us to infer the phased genotype of this individual. An advantage of such imputation approaches is that it saves on sequencing costs while still providing the genotype data essential to many downstream applications. However, these algorithms were developed for use in human populations, and have thus far only been applied in select model systems such as humans and domesticated crops. Importantly, these systems differ markedly in a few important ways from the non-model systems on which many evolutionary geneticists work: they typically have lower effective population sizes, lower levels of genome-wide genetic diversity, and lower levels of recombination. Given these different evolutionary genetic parameters, it is currently unknown whether and how well existing imputation algorithms work in non-model systems, how they compare to one another, and the range of parameters that optimize their performance in a given system.
For this project, we will leverage a recently developed haplotype reference panel in the non-model system white clover (Trifolium repens) to compare and contrast various imputation algorithms (e.g., BEAGLE, STITCH, GLIMPSE) for their ability to correctly impute the genotypes of low-coverage individuals. For each algorithm, we will additionally see how well they perform when using reference panels of varying sizes, and for various sequencing depths of the low coverage target samples being imputed. The goal is to provide a roadmap for genotype imputation in non-model systems whose evolutionary genetic parameters differ from those on which these algorithms are typically applied.
Research area, student roles & skills
Research area: I am a plant evolutionary geneticist who's work focuses on understanding phenotypic and genomic adaptation to contemporary, human-induced environmental change. My lab uses large-scale experiments with plants to understand to selective agents operating in natural plant populations, and combines this with high-throughput whole genome sequencing to uncover the genetic architecture of phenotypes driving patterns of adaptation in nature. In doing so, we leverage the latest computational advances to analyzing genomic sequencing data, and often perform simulations to understand the limits of what existing tools can do, which is especially important when working in non-model systems.
Student roles: The student will be involved in every aspect of the project. Specifically, the student will:
1. Develop a Snakemake pipeline that executes all existing imputation algorithms to impute the genotypes of over 3,000 low-coverage whole genome sequences onto the white clover reference panel that contains around 600 samples. 2. Downsample the reference panel to varying sizes, re-running the imputation algorithms each time to see how well they perform under varying sizes of the haplotype reference panel. 3. Downsample the target samples to varying coverages to see how the imputation algorithms perform under varying sequencing depths.
This project will provide the student with a detailed overview of cutting-edge methods in evolutionary genomics, particularly in non-model systems. At each step of the process, the student will develop ways to assess the false positive rate and accuracy of the different imputation algorithms and report on which performed the best under varying regimes of sequencing depth and reference panel size. I will be around all summer and the student and I will work closely together on this project, aiming for at least on in-depth meeting per week to discuss project progress.
Skills required: Training in genetics, genomics, and bioinformatics would be an asset for this project. In particular, the ideal student would have at least a basic understanding of the basic file formats for genomic data (e.g., BAM, VCF) and the tools that are used to generate and manipulate them (e.g., Samtools, bcftools). In addition, basic programming abilities in Python and Unix-like environments (i.e., command-line and Bash) would be valuable, as most of the work will be performed on a computational Linux cluster. Knowledge of pipeline management software (e.g., Snakemake) would be an asset.
20. Contactless Blood Circulation and Blood Pressure Monitoring
Supervisor: Alexandre Douplik
University: Toronto Metropolitan University
Location: Toronto, Ontario
Start date: 2027-05-17 (flexible)
Disciplines: Biological Sciences, Biology, Computer Science, Electronic Systems, Engg-Biological, Engg-Biomedical, Engg-Computer, Engg-Electrical, Engineering, Medicine, Medical Sciences, Physics, Physiology, Science and Technology, Statistics, Studies Science and Technology
The project is devoted to developing remote touchless monitoring of blood pressure, pulse wave propagation velocity, heart rate, heart rate variability and respiratory rates. This technology is important for ICUs, long-care facilities, and clinics during the pandemic, where breathing and circulatory conditions are critical to control. The hardware/software prototype that was created can perform monitoring 24/7, even overnight, exploiting a low-power near-infrared light source. This project is progressing in collaboration with St. Michael's Hospital, Toronto and Kagoshima University Hospital, Japan. An example of data collection of spatially resolved 2D pulse wave monitoring: Pulse Transit Time (PTT) is determined as the maximum of the cross-correlation between 2 segments of video-recorded tissue to calculate Pulse Wave propagation velocity (PWV), and the blood pressure is computed via further transforms of the data.
Research area, student roles & skills
Research area: Dr. Douplik is a Professor, Head of the Photonics Group in the Physics Department at Toronto Metropolitan (formerly Ryerson) University since 2011, a Scientist at St. Michael’s Hospital, Toronto, specializing in Biophotonics and Laser Medicine, author of more than 70 peer-reviewed papers, 55 conference papers, seven book chapters and 18 patents. He was a visiting researcher at the Okinawa Institute of Science and Technology (OIST), Japan, in 2017, 2018 and 2023. From 2007-2011 he was a contract Research Professor at Erlangen University, Germany, where he defended his Biomedical Engineering Habilitation (Dr. of Science degree) in 2011.
Student roles: Experimental work, data processing and analysis, report writing, and participation in publication.
Skills required: Experimental Design, Optics, Physics, Engineering, Programming, Signal Processing
21. Contribution of HIF-1 alpha in fibrotic responses in gingival fibroblasts
Supervisor: Andrew Leask
University: University of Saskatchewan (Saskatoon campus)
A consequence of fibrosis is hypoxia. We are investigating the mechanisms by which hypoxia can generate fibrosis, and are focusing on the transcription factor hypoxia-inducible factor-1alpha (HIF-1alpha). We are using a combination of approaches to knock down HIF-1alpha (targeted siRNAs in vitro and conditional knockout mice in vivo) to assess the contribution of HIF-1alpha to fibrotic responses to TGFbeta1 in cultured cells and bleomycin-induced fibrosis in vivo. The student, supervised by a PhD student, will assist in the molecular and/or histological characterization of the fibrotic responses using real time polymerase chain reaction to assess mRNA expression or histochemical techniques to examine extracellular matrix deposition.
Research area, student roles & skills
Research area: Fibrotic conditions, responsible for ~45% of the deaths in the developed world, are a major unmet medical need. Fibrosis--excessive scar tissue formation resulting in organ dysfunction--affects the oral cavity. Examples of such conditions are gingival overgrowth and scleroderma. There is no approved drug treatment for fibrotic disease. Our objective is to uncover the mechanisms underlying fibrosis with the aim of developing targeted anti-fibrotic therapies.
Student roles: The student will conduct: a) real time PCR analysis of RNA, extracted from treated cells, to detect expression of fibrotic markers b) histological stains to detect collagen deposition in tissue sections taken from mice exposed to fibrosis
Skills required: Prior experience with cell culture, RNA analysis or microscopy preferred but not required. Prior lab experience recommended.
22. Creating the first nation-wide inventory of public and quasi-public coastal access in Canada
Supervisor: Hannah Harrison
University: Dalhousie University (Halifax campus)
Location: Halifax, Nova Scotia
Start date: 2027-05-17 (flexible)
Disciplines: Biological Sciences, Computer Science, Ecology, Environmental Studies, Geography, Geomatics, Human Ecology, Information Studies, Planning, Sociology
The Coastal Access Project is an ongoing collaborative, interdisciplinary project interested in better understanding, and advocating for, high-quality public coastal access. Amongst other methods, this project uses GIS tools to map the Canadian marine coastline for the purpose of determining what property types make up the coastal zone and, by extension, whether or not those property types allow for public access to the coast. This project builds off a small legacy of student and faculty collaboration that has led to the refinement of mapping methods and the successful mapping of Nova Scotia's shoreline. Now, we hope to build from this success by supporting a new student collaborator to help us build the first ever nation-wide coastal access inventory in Canada. This project would create a clear picture of how much public coastal access actually exists in the country with the world's longest coastline, and lay critical groundwork for answering questions like "which economic classes of Canadians have greatest access to the coast?" and "is access equitably distributed?" and "how will coastal access change as shorelines are developed and sea levels rise?". Our aim is to test and expand our current methodology and make it freely available worldwide to others who wish to contribute to an eventual global inventory of coastal access. In short, this project is ambitious and high impact, but also achievable by our highly collaborative and supportive team.
Research area, student roles & skills
Research area: The research area for this project is coastal access, or the study of how people get to and move along the coastline. Coastal access is a growing field of research that includes topics of mapping, climate change, coastal zone management, equity, planning, policy and governance, and social conflict. This research area studies key questions related to how and why people seek out access to the coastline (in Canada and around the world), the barriers they face in doing so, how shoreline development and climate change may change access in the future, and how access can be protected, enshrined, and maintained.
Student roles: The student will join the FishPeoplePlace lab at Dalhousie University as a member of the Coastal Access Project. They will work directly with Dr. Hannah Harrison, Dr. Patricia Manuel, and students in the Master of Marine Management program to learn project methods and develop the scope of their internship work. They will be responsible for working collegially and respectfully with a diverse group of student and faculty scholars, and contributing to the coastal access inventory project. They will learn and refine the established project methods, then use GIS tools to build coastal maps of the Atlantic Provinces. As time allows, they will be encouraged to work westward across the country. The student will be responsible for producing finished maps of their work and provide basic statistics on the land types they identified. They may also contribute to scholarly manuscripts as a co-author for publication in reputable peer-reviewed journals. Finally, they will produce a short, plain-language report on their work for sharing on the project website.
The student will also be encouraged to take part in lab activities related to coastal access, including (as support is available) ground truthing of mapped areas, taking part in coastal advocacy working groups with local NGO partners, taking part in lab learning activities with other students, and giving a presentation about their results at the end of the internship period.
Skills required: Required skills include: strong GIS and mapping experience, strong written and oral communication skills (English), the ability to work independently and in a lab environment with other students and faculty, strong self-motivation and creativity in problem solving, critical thinking, ability to work respectfully and collaboratively with others of diverse backgrounds.
Educational background: natural (biology, ecology) or social sciences (geography, human ecology, social studies). Other relevant fields of previous study are also welcome (i.e., planning).
Assets: willingness to travel to the field to groundtruth mapping project (1-2 day trips, if funding permits); previous experience working on coastal topics;
23. Cumulative effects of water browning and hypoxia on juvenile salmonids
Freshwater in the Northern Hemisphere is experiencing an increase in hypoxia and in colored dissolved organic matter (CDOM), creating the browning phenomenon. CDOM has the optical property of absorbing blue and ultraviolet radiation. Fish behavior is based on the interpretation of multiple sensory stimuli, primarily visual and mechanical (lateral line). For salmonids, polarized ultraviolet vision is thought to be involved in orientation during juvenile stages. In some salmonids, rheophilic ecotypes in rivers occupy habitats with high flow velocities. Effective station holding becomes imperative to guard feeding territories. This is the case for Atlantic salmon, a culturally and economically important species in Quebec, Canada. Juveniles hold stations where they intercept drifting prey. These territories are subject to intense competition between individuals.
Studies demonstrate instability of rheotaxis during periods of low light in some species. Indeed, visual stimuli allow individuals to orient themselves in space based on their position in a fixed or moving environment. By disrupting one of the key elements of orientation, it is possible that the CDOM alters rheotaxis in salmonids. This may lead to a decrease in critical swimming speed, stability of positioning and station-keeping, or a disruption of swimming behaviors. However, no studies have addressed these issues.
The objective of this study will be to describe and quantify the effect of CDOM as a component of the optical habitat on critical swimming speed using the incremental technique, on station-holding behavior and stability within a swimming tunnel using video imaging.
Research area, student roles & skills
Research area: Dr. Enders is primarily interested in fish ecology, fish and habitat conservation and restoration, and environmental impact assessments of anthropogenic flow and climate changes on fish populations. The Riverine Fish Ecology Lab of the INRS – Centre Eau Terre Environnement has broad research interests in conservation biology, ecophysiology, fish behaviour and bioenergetics, and aquatic ecology to provide scientific advice for Species at Risk and Fish Habitat Protection. Freshwater fishes are used as research models for experiments conducted in both the laboratory and the field.
Student roles: In this lab study, the role of the student will involve to actively participate in divers research tasks. The student's responsibilities begin with a thorough understanding of the study objectives, research questions, and relevant background information. This familiarity will enable the student to grasp the context and purpose of the field work, align their efforts accordingly, and contribute effectively to the overall research goals.
Prior to embarking on the work, the student will play an important role in preparing the necessary equipment and field gear. This involves organizing and gathering the tools, instruments, and materials required for data collection and field experiments. By assisting in this preparation process, the student ensures that all the necessary resources are available and in proper working condition, facilitating a smooth and efficient study.
In the laboratory, the student will actively engage in data collection and lab experiments. This includes following established protocols and methodologies, implementing planned procedures, and conducting observations or measurements as required. The student will conduct behavioral experiments. The student's active involvement allows for the collection of reliable and accurate data, forming the foundation for the research findings and conclusions.
In summary, the student's role in this study encompasses familiarizing themselves with study objectives and background information, assisting in the preparation of experimental setup, actively participating in data collection and lab experiments, taking accurate notes, and ensuring the quality and integrity of the collected data. By fulfilling these responsibilities, the student contributes to the success of the research endeavor and gains valuable hands-on experience in their field of study.
Skills required: To participate in this laboratory study effectively, we encourage students with an educational background in the field of biology or environmental sciences or equivalent degree (e.g., ecology, wildlife biology, natural resource management, or other relevant discipline) to apply. We are looking for a creative and self-motivated student who has critical thinking and problem-solving skills. The student should be able to work effectively both independently and collaboratively in a team. Adaptability is another important skill for this study.
24. Data Science and Microbiome Analysis for Antimicrobial Resistance in Livestock
Supervisor: Nisha Puthiyedth
University: Thompson Rivers University (Kamloops campus)
Antimicrobial resistance (AMR) is a growing global public health challenge affecting both human and animal health within a One Health framework. In livestock systems, understanding the distribution and diversity of antimicrobial resistance genes (ARGs) within microbial communities is important for improving antimicrobial stewardship, surveillance, and management practices.
This research project focuses on the computational analysis of AMR in beef cattle microbiomes using metagenomic and associated metadata. The project aims to investigate microbial community patterns and ARG profiles in cattle-associated microbiomes through bioinformatics and machine learning approaches. The student will work with curated genomic and microbiome datasets to identify resistance-associated patterns, explore relationships between microbial composition and ARG prevalence, and evaluate computational methods for AMR prediction and analysis.
The internship will involve multiple stages of computational research, including data preprocessing, exploratory microbiome analysis, feature engineering, statistical analysis, and implementation of baseline machine learning models such as logistic regression, random forest, and comparative predictive approaches. The student will also conduct controlled computational experiments to evaluate model performance, reproducibility, and interpretability under different analytical conditions.
In addition, the student will generate data visualizations, comparative analyses, and technical documentation to support interpretation of microbiome and AMR-associated findings. The project forms part of an ongoing interdisciplinary research program focused on developing scalable computational tools and AI-assisted workflows for AMR monitoring, microbiome analysis, and One Health research applications.
Expected outcomes include curated computational workflows, cleaned and annotated datasets, preliminary predictive modelling results, visual analytics, and a final research report summarizing findings and future research directions.
Research area, student roles & skills
Research area: My research focuses on computational bioinformatics and data science approaches to study antimicrobial resistance (AMR) in livestock systems, particularly beef cattle microbiomes. I work with metagenomic sequencing data to analyze antimicrobial resistance genes and microbial community patterns across cattle-associated samples. My research integrates machine learning, statistical modelling, and reproducible computational workflows to better understand AMR dynamics and support data-driven decision making in agriculture and One Health research contexts.
Student roles: The student will contribute to a computational research project focused on antimicrobial resistance analysis. They will work with structured datasets to perform data cleaning, organization, and exploratory analysis. The student will implement baseline machine learning models to identify patterns in antimicrobial resistance genes and evaluate model performance. They will also conduct controlled computational experiments, such as testing different model parameters and comparing results across datasets. The student will generate visualizations and summaries to help interpret findings and communicate results clearly. Throughout the internship, the student will follow reproducible research practices, including code documentation and version control. Regular meetings with the supervisor will provide guidance, feedback, and support. The student will also read selected research papers to understand the project context and contribute to a final report summarizing their work and key outcomes.
Skills required: Basic programming experience in Python or R is required. Familiarity with data analysis, statistics, or machine learning is helpful but not mandatory. Students with a background in computer science, bioinformatics, data science, or a related field are encouraged to apply. Strong problem-solving skills, attention to detail, and willingness to learn new tools are important. Prior experience with biological data is an asset, but not required.
25. Deep Learning and Large Language Models (LLMs) for Spatiotemporal Multi-Omics Integration in Personalized Medicine
Supervisor: Abedalrhman Alkhateeb
University: Lakehead University (Thunder Bay campus)
Most multi-omics models ignore where (tissue region) and when (disease stage) molecular changes happen. This project will develop a deep learning framework combining:
1)Graph convolutional networks (GNN) for spatial dependencies
2)Temporal convolutional networks (TCN) for time-series omics
3)Fine-tuned small LLMs (e.g., BioBERT-style) to integrate clinical notes with molecular data
Two interns will:
- Preprocess public spatiotemporal multi-omics datasets (cancer, diabetes, COVID-19) using Seurat, Scanpy, and STAR.
- Build and train a spatiotemporal GNN-TCN hybrid model.
- Validate interactions via KEGG, STRING, and AlphaFold3 predictions.
The final output will predict patient-specific drug responses or disease progression trajectories.
Research area, student roles & skills
Research area: My lab builds AI models to integrate multi-omics data (genomics, transcriptomics, proteomics, metabolomics) with spatial (tissue, cell, organ) and temporal (disease stage, time point) dimensions. We develop graph neural networks for spatial molecular interactions, temporal convolutional networks for progression forecasting, fine-tuned LLMs for clinical knowledge integration, and simulation frameworks for biological validation. We also explore quantum-inspired methods for complex interaction modeling. Our goal is to predict disease trajectories, drug response, and treatment outcomes, advancing personalized medicine by moving beyond "one-size-fits-all" to patient-specific therapeutic strategies based on molecular profiles.
Student roles: Intern 1: Data preprocessing, quality control, and spatial graph construction. Intern 2: Implement TCN and LLM integration modules. Both interns: - Run experiments on Lakehead University’s HPC cluster. - Validate model predictions against biological databases. - Co-author a conference paper or technical report.
Skills required: Python, PyTorch/TensorFlow, basic biology or omics familiarity (transcriptomics/proteomics). Experience with GNNs or time-series models is a plus.
26. Defining and targeting drug toxicity pathways in vitro
Supervisor: Amit Bhavsar
University: University of Alberta (Edmonton campus)
Cisplatin is a highly effective chemotherapeutic widely used to treat solid tumours in children. Unfortunately, over half of treated children develop treatment-related toxicities. Ototoxicity is a frequent adverse drug reaction to cisplatin treatment that causes permanent hearing loss in both ears. This can have a significant impact on speech and language development in children, as well as a prolonged socio-economic impact.
This research project will study cisplatin toxicity using in vitro models. Cellular responses to cisplatin treatment will be characterized in cultured ear cell lines. These responses will be used as reporters of cisplatin-signaling. Drug-gene interactions, e.g. transcriptomic changes induced by cisplatin treatment, will be used to identify genes and pathways required for cisplatin signaling that may contribute to the development of ototoxicity. Signaling components identified through these analyses will be validated by gene silencing and tested in the cisplatin response assays characterized above. Genes critical to cisplatin signaling will be examined as candidates for the development of protectant therapies that prevent the development of ototoxicity. Existing small molecules targeting these candidate genes will be leveraged in this regard.
Otoprotective agents are in demand given the high rates of ototoxicity in cisplatin-treated patients but currently none are approved for clinical use. Otoprotectants identified through this research project would be fit for preclinical animal studies of cisplatin-induced ototoxicity and would serve as a proof-of-principle for this research program.
Research area, student roles & skills
Research area: The Bhavsar laboratory’s specialized area of research is in pattern recognition receptors. One area we are particularly interested in is the mechanisms underlying adverse drug reactions frequently encountered with chemotherapeutics used in childhood cancer treatment. We particularly focus on cisplatin induced hearing loss and examine the role of innate immune signaling, cisplatin metabolism and proteolysis as contributors to this ADR. The goal of the research program is to discover new genes and pathways involved in the development of adverse drug reactions and leverage these insights into strategies to potentially improve the safety of children’s cancer treatments.
Student roles: Non-laboratory roles: The student will be required to read relevant background literature and experimental protocols. A written report of the research project will be expected at the end of the 12-week period that will remain with the principal investigator. In addition, the student will be responsible for recording any new protocols developed in the course of their project. The student will be required to create a LinkedIn/Researchgate profile that includes the laboratory in their network. The purpose of this is to enable their development as highly qualified personnel (HQP) to be referenced in grant applications.
Laboratory roles: The student will work in the lab under partial supervision of a senior graduate student or the principal investigator. The student will be expected to conduct tissue culture experiments and in vitro cell-based assays. In addition to performing experiments, after appropriate training, the student will be expected to perform data analysis to be presented to their supervisor and/or at lab meetings. The student will also be assigned routine lab “jobs”, particularly those pertaining to the equipment/resources used in their project.
Skills required: The student should have a background in biological sciences and be familiar with molecular biology and biochemical concepts. Experience with tissue culture and cell-based assays would be an asset. The student must be able to effectively communicate and must be well-organized. The student should be comfortable with, and have the ability to, work largely independently after initial training. A high degree of critical thinking skills is expected.
27. Developing Intelligent AI Tools for Bioinformatics Learning and Genomic Research
Supervisor: Nisha Puthiyedth
University: Thompson Rivers University (Kamloops campus)
The proposed research project focuses on developing an AI-assisted bioinformatics learning and research platform using locally hosted and open-source Large Language Models (LLMs). The project aims to improve accessibility, usability, and educational support for students and researchers working in bioinformatics, genomics, microbiome science, and antimicrobial resistance (AMR) research.
The research will explore the fine-tuning and evaluation of open-source LLMs such as LLaMA and Phi models using publicly available scientific literature, educational resources, and bioinformatics datasets. The project will investigate how AI-assisted systems can support genomic data interpretation, microbiome analysis guidance, bioinformatics workflow assistance, scientific literature summarization, and automated code generation for computational biology applications.
A major component of the project involves developing a secure web-based platform that enables students and researchers to interact with locally hosted AI models while maintaining data privacy and transparency. The system will support document-based question answering, research paper summarization, workflow recommendations, educational tutoring, and AI-assisted support for bioinformatics pipelines and genomic analysis tasks.
The proposed research combines artificial intelligence, educational technology, and computational biology to address growing challenges in bioinformatics education and research accessibility. The project will evaluate system performance, usability, and educational effectiveness using both quantitative and qualitative assessment methods.
This research is expected to contribute toward scalable AI-assisted bioinformatics education tools, reproducible computational workflows, and privacy-conscious AI deployment strategies for academic and research environments. Findings from the project will be disseminated through peer-reviewed publications and conference presentations.
Research area, student roles & skills
Research area: I conduct interdisciplinary research in artificial intelligence, bioinformatics, large language models (LLMs), and educational technologies. My work focuses on developing AI-assisted systems for genomic analysis, microbiome research, automated learning support, and data-driven educational applications. I have supervised numerous student research projects leading to publications and applied research outcomes. My research integrates machine learning, natural language processing, bioinformatics pipelines, and scalable web technologies to develop practical, privacy-conscious AI solutions for healthcare, computational biology, and higher education environments.
Student roles: As part of the research team, the student intern will contribute to the development and evaluation of AI-assisted bioinformatics and educational support systems using locally hosted and open-source Large Language Models (LLMs). The student will participate in multiple phases of the project, including AI model evaluation, software development, bioinformatics workflow integration, and system testing. Initially, the student will assist in fine-tuning and evaluating open-source LLMs such as LLaMA and Phi models using publicly available scientific literature, educational resources, and bioinformatics datasets. The student will help assess model performance for tasks such as research paper summarization, genomic data interpretation, workflow assistance, automated code generation, and question answering related to computational biology and microbiome research. The student will also contribute to developing a secure web-based application that integrates the locally hosted AI models into an interactive educational and research platform. Responsibilities may include backend development, interface design, API integration, prompt engineering, testing, and user experience evaluation. In addition, the student will participate in data analysis, documentation, preparation of technical reports, and dissemination of findings through conference presentations or peer-reviewed publications. Throughout the internship, the student will gain hands-on experience in artificial intelligence, bioinformatics, software development, and interdisciplinary research while contributing to scalable and privacy-conscious AI solutions for education and computational biology research.
Skills required: The ideal student should have a background in computer science, bioinformatics, data science, computational biology, or a related field. Experience with programming languages such as Python or R and basic data analysis skills is preferred. Familiarity with machine learning, artificial intelligence, or bioinformatics tools would be an asset. Students with experience or interest in large language models (LLMs), natural language processing, or AI-based applications are encouraged to apply. Knowledge of Linux environments, GitHub, or data visualization tools would also be beneficial. The student should be curious, motivated, and interested in interdisciplinary research that combines AI, bioinformatics, and computational biology.
28. Development of 3D systems for organoid culture
This project involves development and optimization non-animal derived biomaterial/hydrogels for organoid culture. Natural and synthetic biomaterials/hydrogels will be screened for biocompatibility and the suitable biomaterial will be developed to culture model organoids such as colon organoids. Colon organoids cultured on conventional 3D matrices will be used as a control and will be compared with the organoids on the new biomaterial/hydrogel systems for gene and protein expression, cell types and histology analysis.
Research area, student roles & skills
Research area: Engineering and bioproduction of recombinat proteins, single cell microfluidic technologies (RNA), Biomaterials and organoid culture.
Student roles: The students participating in this project will: 1. Screen and optimize biomaterial/hydrogel cross linking reactions 2. Evaluate biocompatibility of the suitable hydrogel using established cell lines 3. Develop and optimize colon organoid culture on the new biomaterial/hydrogel 3D system 4. Perform molecular characterization and validation studies. This hands-on experience will provide students with valuable skills in hydrogel crosslinking reactions, organoid culture and different molecular characterization studies.
Skills required: Educational background: Biotechnology, Chemical engineering, Biomedical Engineering, Molecular Biology and other related domains. Laboratory skills: Familiarity with basic laboratory techniques such as pipetting, buffer preparation, pH adjustment, cell culture etc. Analytical techniques (preferred but not mandatory): ELISA and SDS-PAGE are advantageous. Data analysis and documentation.
29. Development of a Sustainable Algal–Bacterial System for Nutrient Removal and Resource Recovery from Wastewater
Supervisor: Qiuyan Yuan
University: University of Manitoba (Winnipeg campus)
This research project focuses on developing and evaluating sustainable technologies for nutrient removal and resource recovery from wastewater. Excess nitrogen and phosphorus in wastewater can cause eutrophication in lakes and rivers, while conventional treatment methods often require high energy input and significant chemical use. The project will investigate an environmentally friendly biological treatment approach that can improve nutrient removal efficiency while reducing operational demands.
The proposed work will examine the performance of an algal–bacterial or other advanced biological treatment system under controlled laboratory conditions. The system will be tested for its ability to remove key contaminants, including ammonia, total nitrogen, phosphorus, and organic matter, from wastewater. Different operating conditions, such as retention time, aeration, light exposure, and nutrient loading, may be studied to determine the factors that most strongly influence treatment efficiency and process stability.
A major goal of the project is not only to improve wastewater treatment performance but also to explore opportunities for resource recovery. The biomass or nutrient-rich by-products generated during treatment may have value for reuse, such as fertilizer recovery, bioresource production, or other beneficial applications. By linking treatment with recovery, the project supports the broader goals of circular economy and sustainable infrastructure development.
The research will involve laboratory reactor operation, routine sampling, water quality analysis, and data interpretation. The student intern will gain practical training in experimental design, environmental laboratory methods, and research communication. The expected outcomes include a better understanding of system performance, identification of optimal operating conditions, and recommendations for future scale-up or industry application.
Overall, this project addresses an important environmental challenge by advancing low-energy, sustainable solutions for wastewater treatment. It will contribute new knowledge to the field of environmental engineering while providing valuable hands-on research experience and strengthening collaboration between academia and industry.
Research area, student roles & skills
Research area: My specialized research area is environmental engineering, with a focus on sustainable wastewater and solid waste treatment. My research investigates innovative biological processes for nutrient removal and recovery, including aerobic granular sludge, algal–bacterial systems, and resource recovery from wastewater. I also work on landfill gas mitigation, biocover systems, and the beneficial reuse of waste-derived materials such as tire shreds and bio-based composites. More recently, I have explored mycelium-based biomaterials and other circular economy solutions. The overall goal of my research is to develop practical, low-energy, and environmentally responsible technologies that improve waste management, water quality, and long-term sustainability.
Student roles: The student will play an active and important role in the successful completion of the research project. Under the supervision of the academic advisor and the graduate student mentors, the student will contribute to both the experimental and analytical components of the study. The student will assist with setting up and operating laboratory-scale treatment systems, preparing materials and solutions, and conducting routine monitoring of experimental conditions. They will participate in wastewater sample collection, preservation, and analysis using standard laboratory procedures. This will include measuring parameters such as ammonia, phosphorus, chemical oxygen demand, pH, dissolved oxygen, and solids concentration. The student will also help maintain accurate laboratory records and ensure that all work is carried out safely and according to established protocols. In addition to laboratory work, the student will be involved in data organization, preliminary interpretation of results, and preparation of figures and tables to summarize findings. They will participate in regular research meetings to discuss progress, challenges, and next steps. The student will also be expected to review relevant literature to better understand the scientific context of the project and to support the interpretation of experimental outcomes. As the project progresses, the student will contribute to the preparation of a final report and may assist in developing presentation materials to communicate the research results to the academic team and project partner. Throughout the internship, the student will gain training in experimental design, environmental laboratory methods, data analysis, and research communication. Overall, the student’s role is to function as a developing researcher who supports day-to-day project activities, contributes to problem-solving and analysis, and gains valuable practical experience in sustainable wastewater treatment research. This role will help the student build technical, analytical, and professional
Skills required: The ideal student should have a background in civil, environmental, or biosystems engineering, environmental science, microbiology, or a related field. Familiarity with wastewater treatment processes, environmental laboratory procedures, and basic data analysis would be an asset. The student should be motivated, organized, and able to work both independently and as part of a research team. Experience with sample collection, chemical analysis, and laboratory safety is desirable but not essential. Strong communication skills, attention to detail, and a willingness to learn new experimental techniques are important. An interest in sustainability and applied environmental research is highly preferred.
30. Discovering the diversity of diving beetles in eastern Canada
Supervisor: Jennifer Perry
University: St. Francis Xavier University (Antigonish campus)
Freshwater invertebrates – including insects – are critical foundational species in wetland ecosystems. Yet, for most freshwater ecosystems, we have only limited understanding of what insect species are present, of whether insect populations are stable or declining, and how insects respond to the warmer water temperatures that are part of climate change. In this project, we will investigate diversity within one group of freshwater insects – diving beetles (Dytiscidae) – in Canada’s east coast wetlands. The objectives are:
1. To sample diving beetles at 20 sites representing diverse freshwater habitats in Nova Scotia, Canada;
2. To identify diving beetles using taxonomic keys and consultation with taxonomic experts;
3. To characterize variation in reproductive structures among populations;
4. To test how variation in species composition, population density, and morphology relate to geographic coordinates, altitude, and depth of water body, because these variables are related to water body temperature.
Methods. The intern will work with a team of graduate and undergraduate student researchers to accomplish the objectives. Sampling diving beetles will involve sweeps with dip nets and baited traps. Reproductive structures will be assessed using both light microscopy and scanning electron microscopy. Training will be provided in field work techniques and safety, insect identification, microscopy, and statistical analysis.
Training environment. The intern will join a supportive and collegial research team. The faculty supervisor will provide mentoring in weekly individual sessions.
Research area, student roles & skills
Research area: Our research group studies insect behaviour, ecology and evolution. We study how insect reproductive behaviour is affected by the local environment – including the warming climate and climate extremes – and how well insect populations adapt to local conditions. We focus on freshwater insects, and aim to characterize species diversity, population divergence in morphology and behaviour, and how reproduction is influenced by increasing temperature. We undertake both field sampling and laboratory studies, and the methods we use include the automated tracking of behaviour, species distribution modelling, physiological assays, population genetics, phylogenetic comparisons, and field surveys.
Student roles: The intern will assist a research team with: - Sampling diving beetles in freshwater habitats, including wearing waders to enter water bodies, using nets to collect insects, - Gathering data on environmental conditions, including keeping meticulous records of location, number of samples, and water body depth - Processing insects in the laboratory, including species identification, preservation and record-keeping - Imaging insect specimens and measuring morphological structures from images - Conducting basic statistical analysis, with support from team members
Skills required: The intern should have a background in ecology, evolution, and entomology. They should be able to distinguish the major orders of insects. Specialized insect identification skills are not required; training will be provided. The intern should be comfortable with working outside in freshwater habitats (e.g., rivers, marshes). They should have very good teamwork skills, including clear communication and reliability. A drivers’ license is helpful but not essential.
31. Dissecting Post-Transcriptional Regulatory Networks in Development and Pathology
Gene expression is governed not only at the transcriptional level but also through extensive post-transcriptional regulation, which is critical for controlling developmental transitions, maintaining cellular identity, and responding to physiological cues. Central to this regulatory layer are RNA-binding proteins (RBPs) and microRNAs (miRNAs), which modulate various aspects of messenger RNA (mRNA) fate, including splicing, stability, localization, and translation. These trans-acting factors exert their effects by directly binding to specific sequence or structural motifs within target mRNAs. However, understanding the precise regulatory functions of RBPs and miRNAs remains challenging due to the complexity and dynamic nature of RNA–protein interactions in vivo. This project aims to develop and refine in vivo techniques to map the RNA-binding activity of RBPs and miRNAs with high specificity and resolution. Additionally, by employing gene editing tools such as CRISPR/Cas9 combined with functional cellular experiments, we aim to dissect the role of specific RBPs associated with pathologies and understand their contribution to gene expression. Our approach will be applied across multiple biological systems, including mammalian stem cells, cancer cell lines, and zebrafish models, to capture both conserved and context-specific regulatory mechanisms. Overall, by linking RNA elements to their cognate RBPs or miRNAs, we aim to construct detailed maps of post-transcriptional regulatory networks and reveal how they contribute to gene expression programs in health and disease.
Research area, student roles & skills
Research area: My research focuses on uncovering the molecular mechanisms that regulate gene expression during organismal development and in diseases such as cancer. I am particularly interested in the roles of microRNAs and RNA-binding proteins in controlling mRNA stability and translation. My laboratory employs a broad range of techniques, including molecular biology methods, biochemistry and cell biology approaches (e.g., CRISPR/Cas9 genome editing), along with next-generation sequencing to study protein–RNA interactions both in vitro and in vivo. We utilize diverse biological models, including mammalian cancer cell lines, stem cells, and zebrafish, to elucidate post-transcriptional regulatory networks in both normal and disease contexts.
Student roles: The student will have their own project and work closely in collaboration with other team members and the principal investigator (PI). During the training, the student will gain extensive hands-on experience in molecular biology techniques, with a specific focus on RNA biology and cell biology. Additionally, basic training in data analysis using Python or R Studio could be provided. The student will also have the opportunity to participate in the Research Center’s activities, such as seminars, invited speaker presentations, and events organized by the student committee of the Center. Finally, the student will take part in regular lab meetings and journal clubs, where they will have the opportunity to present their research progress.
Skills required: We are looking for a motivated student with a background in molecular biology, biochemistry, or a related field. Prior experience in using molecular biology methods, biochemical techniques, or cell culture is an asset but not required. The ideal candidate should be well-organized, capable of carefully documenting experiments, and have a strong desire to learn new concepts and methodologies.
32. Ecophysiological responses of Acaryochloris thomasi to light quality, temperature, and photoperiod
Supervisor: Sylwia Sliwinska
University: Mount Allison University (Sackville campus)
Picocyanobacteria of the genus Acaryochloris are remarkable oxygenic phototrophs that predominantly utilize chlorophyll d, enabling them to harvest far-red light inaccessible to other photosynthetic organisms. Although Acaryochloris marina was discovered more than 30 years ago and a second species, A. thomasi, was described in 2018, the ecophysiology and ecological distribution of this unique group remain poorly understood. Picocyanobacteria are known to exhibit photosynthetic adaptations across a broad range of spectral wavebands, from short-wavelength blue light through green and yellow light to long-wavelength red light. However, little is known about how different light spectra influence the growth and physiological performance of Acaryochloris, particularly A. thomasi. Such information is essential for understanding the spectral niches occupied by these organisms and for predicting their ecological distribution under changing environmental conditions.
The proposed study will investigate the effects of light quality on the growth and ecophysiology of A. thomasi. Cultures will be exposed to eight different light colors (from 405 to 730 nm) representing distinct spectral environments under varying temperature and photoperiod regimes. Physiological responses will be assessed through measurements of growth rates and photosynthetic performance. These experiments will provide novel insights into the ability of A. thomasi to exploit diverse spectral niches and to acclimate to changing environmental conditions. By characterizing the combined effects of light quality, temperature, and day length, this study will improve our understanding of the ecological distribution and adaptive potential of this unique picocyanobacterium. Furthermore, identifying the environmental conditions that maximize growth and photosynthetic efficiency will help predict whether A. thomasi may increase in abundance or become locally dominant as aquatic ecosystems experience shifts in temperature, light regimes, and seasonal patterns associated with global change.
Research area, student roles & skills
Research area: I hold a PhD in Natural Sciences, Earth and Environmental Sciences, and have over 15 years of experience teaching and mentoring students in marine biology and related disciplines. As an experienced marine biologist and Associate Professor, my research and teaching focus on cell biology, microbiology, phycology, algal and cyanobacterial ecophysiology, marine botany, and plant interactions. I also possess advanced skills in R programming and apply statistical and data analysis methods extensively in both my research and teaching. Please check my ORCiD website: https://orcid.org/0000-0002-3147-6605.
Student roles: The student will participate in laboratory investigations of the ecophysiology of unique picocyanobacteria Acaryochloris thomasi, focusing on the effects of environmental factors on its growth and autecology. The student will be responsible for maintaining cultures, preparing media and experimental treatments, conducting measurements of growth and photosynthetic activity, and collecting and analyzing data. Through this work, the student will gain hands-on experience in experimental design, aquatic microbial ecology, and quantitative analysis while contributing to ongoing research on the ecological responses of picocyanobacteria to changing environmental conditions.
Skills required: The ideal candidate will be an undergraduate student with a background in oceanography, marine biology, or a related discipline. The student should possess strong organizational skills, attention to detail, and the ability to work independently as well as collaboratively in a laboratory setting. Basic computer skills and an interest in experimental research and data analysis are expected. Familiarity with the R programming environment for data analysis and visualization would be considered an asset. Previous laboratory experience would also be advantageous, although all necessary training in culture techniques, physiological measurements, and data management will be provided.
33. Effects of environmental pollutants using in vitro assays
The Arctic is traditionally considered a pristine environment, but toxicological research over the past decades has revealed that environmental pollutants used and released in industrialized regions of the world reach the Arctic through long-range transportation processes. Once there, these chemicals accumulate in Arctic food webs and biomagnify to alarming levels in apex predators like polar bears, toothed whales, and seals. These species are exposed to hundreds of legacy chemicals as well as potentially hundreds of new chemicals, termed chemicals of emerging Arctic concern (CEACs). For many of these chemicals we have very little data or understanding of their bioaccumulation (uptake by organisms) and even less regarding their adverse effects (toxicity). Given the large and growing list of environmental chemicals, it is practically impossible to test them all using traditional animal exposure models. As such, we are turning to animal-free cell-based approaches to tackle the issues of chemical testing. In vitro laboratory assays can make use of various cell types, experimental designs, and analytical techniques to characterize the bioaccumulation and toxicity of priority chemicals. The focus of this project will be to develop, test, and apply in vitro assays to characterize the toxicology of CEACs. The student will design and carry-out cell culture experiments with difference cell lines and CEACs and measure bioaccumulation potential and several toxicity endpoints including cell viability, cell-specific function (e.g. immune function for immune cells), receptor binding, and gene expression.
Research area, student roles & skills
Research area: Chemical pollutants are an important threat to the health of wildlife and humans around the world. Pollutants are released to the environment, accumulate in food chain, and can reach high levels of concern in apex predators. The study of environmental toxicology is focused on characterizing the environmental distribution of chemicals as well as their potential for accumulation and toxicity in organisms. This is a gargantuan task given the thousands of new chemicals produced every year that need testing. Use of laboratory based assays (in vitro) is critical for many aspects of chemical risk characterization.
Student roles: The student will help maintain and culture various cell lines that will be used for exposure experiments throughout the summer. The student will design and carry-out cell culture exposure experiments with difference cell lines and environmental chemicals of interest. Experiments will be designed to characterize chemical bioaccumulation and toxicity and the student will measure relevant molecular and cellular endpoints, including cell viability, cell-specific function (e.g. immune function for immune cells), receptor binding, and gene expression. The student will also be expected to analyze the generated data, write summary reports, and participate in resulting manuscript preparation with other lab members.
Skills required: The student should have basic laboratory skills and be comfortable in a molecular biology lab setting. Experience with cell culture and cellular assays is a major asset. Background in toxicology is useful, but not necessary.
34. Engineering the Flavour of Plant-Based Foods Through Fermentation
The growing demand for plant-based foods has created an urgent need to improve their sensory quality and consumer acceptance. One of the major challenges limiting the adoption of pulse- and grain-based foods is the presence of undesirable off-flavour compounds, often described as beany, grassy, earthy, or bitter notes. At the same time, there is increasing interest in developing natural flavour ingredients from sustainable sources, including agri-food byproducts.
Fermentation, particularly solid-state fermentation (SSF), offers a promising and sustainable approach for flavour engineering. Through microbial metabolism, fermentation can reduce undesirable flavour compounds while simultaneously generating desirable aroma and taste molecules. Despite growing interest in this area, the mechanisms through which fermentation transforms flavour precursors and creates novel flavour profiles remain poorly understood. Furthermore, most studies focus primarily on off-flavour reduction, while comparatively little attention has been given to the intentional creation of novel flavour compounds from plant-based materials and food-processing byproducts.
The objective of this project is to investigate how fermentation can be used to engineer the flavour profile of plant-based foods and ingredients. Specifically, the project will evaluate the reduction of key off-flavour compounds and the formation of novel aroma-active compounds during fermentation of pulses, cereals, and selected agri-food byproducts. Advanced analytical techniques, including gas chromatography–mass spectrometry (GC-MS) and nuclear magnetic resonance (NMR), will be used to characterize flavour changes. The outcomes of this work will contribute to the development of more appealing plant-based foods while supporting the valorization of underutilized agricultural resources and advancing sustainable food production systems.
Research area, student roles & skills
Research area: I am an Associate Professor in Food Science at the University of Guelph and lead the Food Processing, Structure and Quality Lab. My research focuses on understanding how food processing modifies food structure and composition and how these changes influence flavour, nutritional quality, digestibility, and techno-functional properties. My research integrates food processing, flavour science, advanced analytical chemistry, digestion studies, spectroscopy, imaging, and data science to develop innovative and sustainable food ingredients and products. Particular emphasis is placed on plant-based foods, fermentation technologies, and the application of machine learning to support next-generation food innovation.
Student roles: The student will contribute to a research project investigating the use of fermentation to improve and engineer the flavour profile of plant-based foods and ingredients.
The project will begin with a comprehensive literature review to identify current research progress, knowledge gaps, and emerging opportunities related to flavour generation and off-flavour mitigation through fermentation. The student will assist in designing experimental approaches to evaluate flavour changes during fermentation of selected plant-based substrates.
The student will participate in laboratory activities involving sample preparation, fermentation experiments, and flavour characterization. A major component of the project will involve method development and analytical measurements using advanced techniques such as gas chromatography–mass spectrometry (GC-MS) and nuclear magnetic resonance (NMR) spectroscopy to profile volatile and non-volatile flavour compounds. The student will also assist in processing and organizing analytical datasets.
In addition, the student will gain experience in multivariate data analysis and chemometrics, including principal component analysis (PCA), clustering methods, and other statistical approaches used to identify key flavour markers and fermentation-driven transformations. The student will work closely with graduate students and researchers in a collaborative environment and will participate in regular research meetings, data interpretation sessions, and scientific discussions.
By the end of the internship, the student will have developed valuable skills in flavour science, analytical chemistry, and data analysis, while contributing to research aimed at improving the sensory quality and sustainability of plant-based foods.
Skills required: The ideal candidate is an undergraduate student with a background in Food Science, Chemistry, Biochemistry, Biotechnology, Chemical Engineering, or a related discipline. Students should have a strong interest in food innovation, fermentation, flavour science, and analytical chemistry. Experience with laboratory research, data analysis, chromatography, spectroscopy, or microbiology would be beneficial but is not required. The successful candidate should be motivated, curious, detail-oriented, and able to work effectively both independently and as part of a multidisciplinary research team.
35. Epigenetic effects of prenatal cannabis and tobacco smoke exposure
Supervisor: Meaghan Jones
University: University of Manitoba (Winnipeg campus)
Prenatal exposure to cigarette smoke in humans leaves behind changes in DNA methylation at a number of specific genes, some of which are likely due to tobacco-specific compounds, but some might be due to inhaled particulate matter in general. Since cannabis was legalized in Canada, we need to better understand the health impacts of cannabis smoke and how these overlap with tobacco smoke. However, it is difficult to determine cellular function changes and health outcomes from human population studies. Thus, we are modeling prenatal cannabis and tobacco smoke exposure in mice and in cells, with the goal of being able to assess timing, outcomes, molecular mechanisms, and other details. This project is foundational to that work, measuring epigenetic marks and/or analyzing epigenetic data from tissues from animals and cells with and without prenatal exposure to smoke.
Research area, student roles & skills
Research area: My lab uses epigenetics to study how prenatal environments and exposures can get under your skin and affect your health. If you imagine that all genes in the genome are lightbulbs, then the epigenome is the dimmer switches on those lightbulbs, and those dimmers can get set in early life in response to specific environmental exposures.
We study how epigenetic marks changes in response to prenatal environmental exposures like cigarette smoke. Epigenetics might help us understand why people who are exposed to cannabis or tobacco smoke in utero or as children are at risk of asthma.
Student roles: For this project, the student will be generating and/or analyzing epigenetic data from a number of different types of tissues and/or cells. The student will work closely with a lab technician or graduate student, receive all training necessary to complete the project, and be encouraged to work with the team, including the PI, throughout their time in the lab. Basic bioinformatics knowledge is required to be able to understand the software used and adapt assays if necessary. The student will work with lab members to test candidate gene expression, DNA methylation, and/or histone modifications, at candidate genes or genome-wide, in already-collected samples, or tissue culture samples generated by the student over the summer. The specific tissue in which this will be done will depend on the student’s interest, but could include blood, whole lung or lung epithelia, liver, or brain. Data analysis will be done in R, with training in R if required, and the student will be expected to give a presentation to lab members at the end of their time in the lab to discuss their findings.
Skills required: Skills needed: Experience in a lab setting Basic molecular biology experience Attention to detail Meticulous record keeping Ability to work independently and in a team Curiosity and willingness to try
Skills gained during the project: Bioinformatics Molecular biology Pyrosequencing and/or sequencing library prep Assay design and validation Reporting Presentation skills
36. Evaluation of Virtual Reality as a Pain Modulation Tool
Supervisor: Ke Peng
University: University of Manitoba (Winnipeg campus)
Pain is a complex and subjective experience associated with actual or potential tissue damage. Despite significant advances in pain research, self-reported measures remain the clinical gold standard; however, they are inherently subjective and often lack precision. Pharmacological pain management, particularly opioid-based treatments, also carries risks including dependence and adverse side effects, highlighting the need for effective non-pharmacological alternatives.
Virtual reality (VR) has emerged as a promising tool for pain modulation. By integrating visual, auditory, and potentially haptic feedback, VR can create immersive environments that may reduce pain perception and related distress. However, reported effects of VR-based analgesia remain variable, and its underlying neural mechanisms are not yet fully understood.
In this project, we will develop a novel platform integrating VR with functional near-infrared spectroscopy (fNIRS), a portable and non-invasive optical neuroimaging technique that measures cortical hemodynamic responses as a proxy for neural activity. The overarching goal is to use fNIRS to objectively characterize brain oxygenation responses during VR-based interventions and evaluate their effectiveness in modulating pain perception.
This project will advance our understanding of the neural mechanisms underlying VR-based analgesia and support the development of personalized pain management strategies by linking VR design parameters to individual neurophysiological responses.
Research area, student roles & skills
Research area: I am an Assistant Professor in the Department of Electrical and Computer Engineering at the University of Manitoba. My research focuses on the development of multimodal brain imaging systems, including functional near-infrared spectroscopy (fNIRS), functional magnetic resonance imaging (fMRI), and electroencephalography (EEG), as well as immersive multimedia technologies such as virtual reality (VR). My work aims to investigate brain function in both healthy individuals and clinical populations, with applications in neurorehabilitation, pain, and cognitive neuroscience.
Student roles: The student will contribute to the project through the following activities: (1) Development of VR-based experimental environments and interactive tasks for pain-related studies. (2) Integration of VR systems with fNIRS acquisition hardware and experimental protocols. (3) Support for data collection in healthy participants. (4) Processing and analysis of fNIRS data, including interpretation of brain hemodynamic responses under different VR conditions. (5) Assistance with dissemination of results, including preparation of conference abstracts and manuscripts for peer-reviewed publication.
Skills required: The student should have background knowledge in at least one brain imaging modality, such as EEG, fMRI, or fNIRS, or prior experience in physiological or neurophysiological data collection. Basic programming skills in at least one language (e.g., C#, MATLAB, or Python) are required. Experience with data analysis, signal processing, or VR development would be considered an asset.
Past MITACS students have worked on a variety of projects and I try to involve the student when deciding on the specific project to make it more meaningful. Most of the projects use DNA markers to look at population genetics either of a single species or closely related species/subspecies. We examine how landscapes and behaviour influence dispersal and can ultimately lead to speciation. Most of the projects are on land birds; however, some are on seabirds. Some of the projects may involve field work, a great way to see the animals in their natural setting and to see a bit of the country. Some of the projects also involve genome sequencing.
Research area, student roles & skills
Research area: Our projects focus on a wide range of questions and species. We do research on landscape genetics, population genetics, bioinformatics, and evolutionary biogeography. Basically we use genetic markers to create a time machine and see what processes lead to the patterns we see in today's species. We are interested in how populations are evolving and what leads to populations becoming isolated.
Student roles: The student will be doing DNA extractions, PCRs, genotyping and sequencing to collect the data. Some of the projects also involve genomics. The techniques are pretty straightforward and easy to learn. They will work with a graduate student to analyze the data. In the past students have been co-authors on conference presentations and some have even presented their findings at conferences.
Skills required: Ideally students have some lab experience and a background in Biology, but it is not required. They must be able to work independently (after training), manage their time, follow instructions, and work in a safe manner. You will be working as part of a team. Genetics is more important than ever in almost every field of Biology, these projects give students a great starting point to learn the genetics as well as get hands on experience developing and working on a project. The projects also allow students to combine field and lab work in a single project.
38. Exploring a Novel Regulator of Gene Expression Controlled by microRNAs
This internship offers an exceptional opportunity to explore the mechanisms of gene expression regulation through the study of novel factors involved in microRNA function in animals. These small non-coding RNAs, highlighted by last year's Nobel Prize in Physiology or Medicine, play a central role in post-transcriptional gene regulation, a process crucial in many biological contexts, from embryonic development to human disease.
As part of a dynamic research laboratory, the student will contribute to a cutting-edge project aimed at elucidating the role of these emerging regulatory factors. The project integrates advanced techniques in genetics, molecular biology, and microscopy, providing a hands-on and versatile training experience. This internship will enable the student to acquire strong technical skills while actively participating in fundamental research with potential implications in molecular medicine and biotechnology.
Research area, student roles & skills
Research area: Our laboratory investigates the role of short non-coding RNAs, particularly microRNAs, as precise regulators of gene expression. These molecules are essential for normal cell function, and their dysregulation is often associated with diseases such as cancer. Some of our projects aim to uncover how microRNAs operate under various biological conditions and identify the mechanisms that control their activity. To achieve this, we use advanced techniques in genetics, molecular biology, and biochemistry. Our research relies on powerful model systems, including the nematode Caenorhabditis elegans and cultured cells, both crucial tools for discoveries in biomedical science.
Student roles: The student will carry out their project in collaboration with a graduate student. During his internship, the intern will learn various cutting-edge techniques in genetics and molecular biology. Their participation in our activities (laboratory meetings, reading clubs) will allow them to learn how to present and analyze research work. They will also participate in our series of seminars and conferences at the center which will improve his/her knowledge on various research themes.
Skills required: -University training in molecular, cellular biology, biotechnology -Curious, independent person with a sense of organization -Prior laboratory experience is an asset
39. Exploring the contribution of peripheral nerves in tumor progression
Supervisor: Anand Krishnan
University: University of Saskatchewan (Saskatoon campus)
This project will examine the role of signaling derived from the peripheral nerves in tumor growth. In particular, the student will determine the levels of neurotransmitters, neurotrophic factors and their corresponding receptors in human tumors, focussing on both cancer and stromal cells in the tumor microenvironment. To execute the project, the student will perform research techniques such as immunohistochemistry, advanced fluorescence microscopy, ELISA, Western Blot and mammalian cell culture. By participating in this project, the student will gain both theoretical and practical knowledge in cancer and neurobiology research.
Research area, student roles & skills
Research area: There is compelling evidence that solid tumors and peripheral nerves interact and mutually support the growth of each other. However, critical molecular players involved in this interaction are not well identified. Full characterization of the tumor-nerve interface and identification of the molecular players involved will reveal novel therapeutic targets for cancer and nerve regeneration. My lab focuses on mapping the tumor-nerve interface to develop molecular targets for cancer and neurodegenerative disorders.
Student roles: The student will perform immunohistochemistry/western blot using human tumor and corresponding normal tissue samples. They will thus determine the expression levels of adrenergic, cholinergic, and neurotrophin receptors in these tumors and corresponding normal tissues (weeks, 1-4). The student will also perform ELISA to determine the levels of neurotransmitter/neurotrophins in these samples (weeks, 5-8). Finally, the student will perform cell culture and evaluate the cell response (cell proliferation/migration/invasion) in response to combined treatments of neurotransmitters/neurotrophins (weeks, 9-12).
Skills required: 1. The student should be enrolled in an undergraduate program in any of the life sciences disciplines and have theoretical knowledge in either cell biology, biochemistry, molecular biology or pharmacology. 2. Basic practical skills in cell culture and molecular biology.
40. Factors Shaping Freshwater Fish Communities Across North America: Glacial History, Environment, and Human Influences
This project investigates the factors shaping beta diversity (differences in species composition) of freshwater fish across North America. Freshwater ecosystems, hosting highly diverse yet increasingly imperiled fish communities, are influenced by a complex interplay of historical, natural, and anthropogenic factors. Understanding how post-glacial colonization, climatic conditions, habitat fragmentation due to dams, land-use changes, and non-native species introductions influence fish diversity is crucial for effective conservation and management.
The project builds on data from my previous research published in Global Ecology and Biogeography (2021. 30:1232-1244). The student will specifically analyze two key components of beta diversity: species richness differences and species replacement. Advanced statistical methods will be employed to identify and quantify the relative impacts of historical events, environmental gradients, and human activities on these biodiversity patterns.
The student will gain practical experience managing spatial ecological datasets and performing sophisticated statistical analyses using R. They will also develop proficiency in Geographic Information Systems (GIS) for environmental data integration and visualization. Additionally, students will enhance their ecological reasoning skills by interpreting complex biodiversity metrics within a biogeographic context and refining their scientific communication through report writing and presentations.
This project offers valuable training for students interested in ecology, biodiversity conservation, biogeography, and quantitative analysis, equipping them with analytical and technical skills highly sought after in research and conservation sectors.
Research area, student roles & skills
Research area: My research integrates aquatic ecology and quantitative ecology, with a focus on understanding and predicting the cumulative effects of human activities on freshwater ecosystems. I use quantitative approaches to explore ecological patterns and processes across multiple spatial scales, from local to continental, and across a range of organisms, from microbes to fish. I am committed to advancing data-driven, policy-relevant science to inform sustainable freshwater resource management.
Student roles: The student will play an integral role in this project, contributing to key aspects of data preparation, analysis, and interpretation. They will compile and prepare data (glacial history, climate, topography, human activities) from multiple sources and conduct a literature review to inform the project’s conceptual framework and support interpretation of results.
A major component of the role involves learning and applying advanced statistical methods to examine how historic, environmental and anthropocentric factors influence fish biodiversity patterns. The student will use R for data analysis and gain valuable experience working with large ecological datasets.
As a supervisor, I am committed to providing a supportive and collaborative learning environment. I will offer regular one-on-one mentorship, guiding the student through all stages of the project from data compilation and analysis to interpretation and scientific writing. The student will also have opportunities to engage with my broader research network and contribute to publications resulting from the project.
Skills required: The ideal student should have a background in ecology, environmental science, biology, geography, statistics or a related field, with an interest in biodiversity conservation and/or quantitative modeling. Strong analytical skills and experience with statistical analysis (preferably in R) are desirable. Familiarity with GIS and spatial data analysis is an asset but not required. The student should be comfortable working with large datasets and have strong written and verbal communication skills. Curiosity, initiative, and the ability to work both independently and collaboratively are essential for success in this project.
41. Fate of contaminants and beneficial compounds in aquatic food webs
Supervisor: Tim Jardine
University: University of Saskatchewan (Saskatoon campus)
The intern will participate in research programs that explore the movement of chemicals through river food webs, including those that are beneficial (e.g. omega-3 fatty acids) and those that are harmful (e.g. mercury, selenium). The first program, funded by the Canadian government, examines the flight of insects from wetlands into rivers where they are eaten by fishes, providing important nutrients to the fishes in the lead-up to the long, harsh winter season. But these wetlands are often surrounded by crop fields, so the insects may be carrying pesticides and other harmful chemicals with them when they move, exposing the river fishes. Graduate students in Dr. Jardine's laboratory are studying various aspects of this migration in all seasons. The second program that is in development will assess bioaccumulation of selenium in river food webs downstream from coal mining activities. It will examine how different selenium species (e.g. selenate, organoselenium) move through food webs, and what risks they may pose to fishes and people. If successfully funded, this program would begin in May 2027 with field work in British Columbia.
Research area, student roles & skills
Research area: Dr. Jardine’s laboratory, which is affiliated with both the School of Environment & Sustainability and the Toxicology Centre, consists of six graduate students and several technicians who collectively conduct applied river research. This team studies contaminant bioaccumulation, the environmental effects of large dams on downstream wetlands, and the basic ecology of rivers and their floodplains. It seeks to use scientific information to engage communities and government agencies in effective stewardship of local aquatic resources.
Student roles: In the interest of exposing the intern to a range of research activities, they will assist where needed during the active summer season. Dr. Jardine’s graduate students, with whom the intern will spend most of their time day-to-day, conduct a range of skill-building activities including: - field work in rivers, lakes and wetlands in western Canada that entails collection of invertebrates and fish using varying sampling methods (dip nets, electrofishing, seine netting, gill netting, etc.) - chlorophyll and nutrient analyses - preparation of fish, bird and mammal tissues (weighing, measuring, dissection, drying and grinding) - encapsulating tissue samples for stable isotope analysis- measurement of mercury in tissues by Direct Mercury Analysis - use of high-tech sondes to measure water quality.
The intern will be given the opportunity to participate in and lead many of the above activities, and will learn novel technologies and procedures including field collection of samples, preservation and extraction of samples, analysis of samples applying a range of state of the art analytical, biochemical, and molecular techniques, as well as data analysis. They will learn how to plan and execute field trips, how to maintain equipment, how to conduct aquatic sampling, and how to keep good records in the laboratory. All of these skills are transferable as they continue in their scientific careers.
Skills required: We require an intern who has an academic background in biology/ecology and who is willing and eager to participate in field work in rivers, lakes and streams. The field work can be challenging, so demonstrated time spent outdoors (e.g. camping) would be necessary. Some experience working with animals would be considered an asset. The intern will also work in the laboratory and will be expected to keep good records and follow standard laboratory protocols.
42. Forest pest biology and parasitoids
Supervisor: Jantina Toxopeus
University: St. Francis Xavier University (Antigonish campus)
While insects are fascinating animals, they can cause economic challenges. The Toxopeus lab is currently studying two forestry pests. The eastern spruce budworm (Choristoneura fumiferana) is native to Canada, but its outbreaks every 30-40 years cause substantial damage to spruce and fir trees in the boreal forests in Canada. The beech leaf mining weevil (Orchestes fagi) is a pest of beech trees in the Acadian forests, and is not native to our region. We are investigating populations of both of these pests, their natural enemies, and mechanisms that can help control their populations.
For work on either of these species, we use field work to identify local populations of the insects, collect data on their local environment (tree species, climate, etc.), bring insects back to the university for laboratory studies or rearing, and identify whether the collected insects are parasitized. Parasitoids are usually wasp species that parasitize the host (e.g., spruce budworm), causing death of the host. They can be a natural source of biological control. This work will identify environmental factors that affect the presence and abundance of our species of interest, and whether biological control via native parasitoid species is a viable path forward.
Research area, student roles & skills
Research area: The Toxopeus lab (https://jantinatoxopeus.com/) focuses on understanding how animals and their cells survive in challenging environments, especially low temperatures associated with winter. This is important for understanding how organisms tolerate current environmental stressors, and how they might respond to future changes in their environment (e.g. due to global climate change), with implications for forestry, agriculture, and human health (e.g., when studying insect pests and vectors of diseases). Our work on freeze-tolerant insects also has implications for understanding cryopreservation, an important technique for preserving cells and tissues for biomedical purposes.
Student roles: The student will learn how to collect insects and environmental data, rear insects and their parasitoids, record the data from these experiments in an organized fashion, and (if interested) develop computational models that help predict insect presence and abundance. The student will also learn how to analyze the data and present it in visual or graphical form to share the information with their supervisor and other scientists. The student will also be expected to spend some time learning about the project by reading scientific literature, and may be expected to contribute to the process of publishing the results from their project. All students in the Toxopeus lab regularly meet with Dr. Toxopeus and participate in group laboratory meetings.
Skills required: Some experience with field work, entomology, or forestry is an asset, but not required. Some experience using the techniques described in the “required role” section would also be an asset, but are not required. Students will be fully trained in relevant background knowledge and techniques for their project while in the Toxopeus lab.
Generally, research projects are most successful when students have strong attention to detail, organization, and time-management skills. It is also important to be able to work well independently and as part of a team. Finally, a general curiosity about the way biology works is always good!
Ecological communities are organized into complex networks of trophic interactions between the species that are part of them, namely food-webs. These webs depict the patterns of energy flow and nutrient cycling among the different compartments of the ecosystem, processes that are essential to ecosystem functioning. Their functioning depends on their structure, including the number, abundance, and functional traits of the species that are part of the network. Hence, any natural or anthropogenic variation (e.g., river damming, habitat type) that affects species distribution changes the way that food-webs are structured and might change, consequently, its functioning.
Modelling the structure and dynamics of food-webs is frequently a hard task given the lack of information about species trophic interactions. To facilitate such as goal, this project aims to build a global-scale open database of predator-prey interactions in freshwaters and assess the macroecological factors underpinning food web structure. This will be initially carried out through a systematic review of the primary literature (articles published in scientific journals) and grey literature (conference abstracts, undergraduate and graduate dissertations) in any language. The review will collect multiple information from the literature, including predator identity and size, prey identity and consumed abundance/volume, geographic location, sampling size, and habitat type. Extracted information will be integrated into the database through a workflow that will automatically fill out a wide range of climate and impact-related variables provided by remote sensing.
This research project will be the starting point to large-scale analyses that will allow us to (i) model the trophic interactions performed by freshwater species in different scenarios; (ii) understand how natural and anthropogenic variation in nature affects predator-prey dynamics; and (iii) predict how future changes will affect the structure and dynamics of freshwater food webs.
Research area, student roles & skills
Research area: My research strives to understand how and why biodiversity varies in space and time, primarily focusing in how the climate, landscape, and human activities determine the structure and functioning of aquatic ecosystems. These determining factors have complex effects over aquatic ecosystems, and might change not only species distribution and local diversity, but also the interaction networks and ecosystem services shaped by them. Therefore, it is crucial to advance the capacity of predicting ecosystem structure and functioning in face of a changing environment in order to subsidize an effective management and conservation of nature.
Student roles: The student will participate in two primary activities: (i) data extraction and (ii) data analysis. Data extraction will follow a standardized protocol, in which the student will be trained before the beginning of the process. A list of references will be provided to the student, and they will read through the manuscript to identify a sequence of information and fill out a database with them. Given that some of the information to fill out the database might require expert judgement, the student will have to often meet or discuss with the supervisor to adjust information. In addition, some steps of the data extraction be required to follow a workflow in R, which will also be part of their training. Finally, the student will be supervised in analyzing the extracted information using a pre-established workflow in R and summarizing the main outcomes of their work in a simple report. By the end of the training period, I expect the students to develop skills in performing data extraction from the primary literature and managing large-scale databases using Excel and R. They will also be introduced to skills related to summarizing a large quantity of information and writing technical reports.
Skills required: The student must have a background in environmental sciences, zoology, or ecology in order to better grasp the goals and nuances of the project. Students with specific research experience in natural history or freshwater ecology are preferred. In addition, students with some practice performing literature reviews and/or using Excel would be preferred given the main tasks they would carry over here. Practice in the use of R for data analysis is a plus, but not mandatory.
44. Functional and mutational characterization of tomato defence genes to develop disease-resistant plants for a warming climate
Supervisor: Christian Danve Castroverde
University: Wilfrid Laurier University (Waterloo campus)
Global warming influences plant disease development by targeting components of the plant immunity. We previously discovered that elevated temperature enhances disease and suppresses defence hormone production in Arabidopsis thaliana plants by negatively regulating a master immunity transcription factor (TF). Elevated temperature suppression of this TF gene expression results in downregulating key genes important for salicylic acid (SA) biosynthesis – a crucial immune signal to activate defences against a broad range of pathogens. We have previously shown that this TF is broadly conserved in the plant kingdom, but homologs in other plant species have yet to be characterized. In the agriculturally important tomato crop, our in silico analyses revealed 11 homologs of this master TF. Three tomato genes showed induced gene expression in response to pathogens. Because detailed functional characterization is lacking, this project aims to address this knowledge gap. We will build on this research by characterizing tomatoes with mutations in these three genes. To this end, mutant plants will be infiltrated with the model bacterial pathogen Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) to induce defence gene expression and immune responses. Samples will be collected and used for defence gene expression profiling and SA hormone quantification. Bacterial growth and disease symptoms will also be measured. With wild-type control plants as reference, tomato mutants with altered defence gene expression, SA levels and/or bacterial susceptibility would shed light on which TF homologs primarily mediate plant immunity. Overall, this project will untangle the evolutionary importance and extensive conservation of the SA pathway in plant immunity. This will make it possible to narrow down tomato defence response genes for CRISPR/Cas9 genome editing and translational crop protection.
Research area, student roles & skills
Research area: My research program is centred around understanding plant immune signalling and disease resistance in a changing environment. The overarching vision is to: (1) discover novel regulators of temperature-sensitive immunity; (2) determine if temperature-regulated immunity is conserved in diverse species; and (3) develop innovative technologies to repair the immune system and generate climate-smart plants. We integrate multiple approaches, from molecular biology and genetics to microbiology and biochemistry, to investigate the profound impact of changing temperatures on plant immunity and disease. It is anticipated that fundamental discoveries can be leveraged towards disease prevention and climate resilience programs in Canada and globally.
Student roles: The student will be involved in experimentation, data collection and data analyses. They will maintain their own tomato plants and initiate Pst DC3000 bacterial cultures for pathogen inoculation assays. They will inoculate plants with pathogen and collect tissue samples along a time course of infection. The student will use these tomato samples for defence gene expression analyses (RNA extraction, cDNA synthesis and qRT-PCR), SA measurements (biosensor luminescence assay) and bacterial growth determination (colony counting).
Skills required: Required: Background in biology, basic lab experience Preferred (but not required): Advanced skills in molecular biology, microbiology and plant biology
45. Gene expression evolution
Supervisor: Malgorzata Gazda
University: Université de Montréal
Location: Montreal, Québec
Start date: 2027-05-03 (flexible)
Disciplines: Biological Sciences, Biology, Genetics, Computer Science, Molecular Biology, Veterinary Science and Medicine
The overall aim of this project is to identify the molecular changes in gene expression patterns in relation to the tissue and treatment exposure. Both the publicly available RNA sequencing data, as well as in house generated RNA libraries will be used to assess the importance of the traits, sex and the response to treatment. Bulk RNA-seq will be applied to the tissue of interest. The patterns of gene expression will be compared across species. Standard bioinformatics procedures will be applied, including demultiplexing sequencing data, quality check and trimming, mapping reads to transcriptome, followed by differential expression and enrichment analysis (to gain insights into affected pathways). This will be conducted together with exploration of gene function in available resources and published datasets (such as mouse knockouts). Using bioinformatics tools we will examine whether those genes are relevant for any health disorders or drug treatment.
Research area, student roles & skills
Research area: In my research group we combine population genomics, classical genetics, biochemistry, physiology, molecular biology, phylogenetics, neurobiology and behavior. The interdisciplinarity is achieved thanks to wide range of collaborations as well as our willingness and courage to explore unknown. We work on wide variety of organisms from birds to plants, with main model species of canary birds. Other than genotype phenotype association we are also interested in understanding how gene function and expression evolve across species as well as how is it impacted by organismal biology, physiology and environment.
Student roles: Candidates depending on their experience and interest will be able to develop the bioinformatics skills, by managing the transcriptomics data, investigating batch effect, mapping, performing gene expression analysis. Additionally there will be opportunity to learn practical skills in molecular biology lab, such as extracting RNA, preparing sequencing libraries, qPCR. Each intern will be expected to write a report based on their projects as well as present it at the group meeting. Potentially the mix of both, so the computer analysis combined with the bioinformatics is also possible.
Skills required: Candidates for the internship should be interested in evolutionary biology. Basics of scripting/basic programming will be beneficial but not required. General understanding of evolutionary biology, genetics and genomics or computer science will be helpful in order to work in an interdisciplinary team with background in biology and computer science. Interest in gene expression evolution will be an asset. Students should be willing to work collaboratively as a part of the team, with professionalism, attention to details and eager to learn.
46. Gene expression patterns in young and old carpenter bees
Supervisor: Miriam Richards
University: Brock University (St. Catherines campus)
The research project combines research in ecology, behaviour, molecular biology, and bioinformatics. Students will develop skills in ecology fieldwork, behavioural observations, dissection and measurement of insect specimens, DNA and RNA extraction, and use of bioinformatics analysis pipelines for differerential gene expression (DESeq) based on transcriptome data (RNASeq). Students will begin with fieldwork in spring to identify male and female carpenter bees (Xylocopa virginica) at their nest sites. Using behavioural observations of individually marked bees, we will identify which males initiate territorial behaviour in spring. We will then collect a set number of territorial males each week while males continue to display territorial behaviour. Males will be flash-frozen in liquid nitrogen, then total RNA will be extracted and sent for sequencing at (RNASeq by TCAG, Toronto). This approach will provide a series of males ranging from young, unworn, undamaged males collected at the beginning of territoriality, to old, very worn, senescent males collected at the end of the season. Additionally, we will collect a set of young males early in the season to be kept alive in the lab in terraria too small to fly in, so they get older without the physical deterioration caused by flight. We will then compare gene expression (transcriptomes) of young males that have accumulated no or very little damage with territorial behaviour, older males with varying degrees of damage due to territorial behaviour, and older males without damage due to territorial behaviour. Preliminary gene expression data already demonstrate that these three groups differ significantly in gene expression patterns. Students will further characterize overall differences in the transcriptomes of different groups of males using DESeq and will also look for evidence of differences in gene expression patterns for specific biochemical pathways related to aging, senescence, lifespan, oxidative stress, and thermal stress.
Research area, student roles & skills
Research area: Male and female animals may senesce at different speeds if their activity patterns have different propensities for tissue, cellular, and DNA damage. In large carpenter bees, males experience more rapid physical senescence and have shorter lives than females, as a consequence of their physiologically demanding territorial behaviour (hovering and fighting in full sun, sometimes at high temperatures). Also, male bees are haploid, lacking DNA repair systems that operate effectively in diploid females. Our lab is testing the hypothesis that the shorter lifespans of male bees reflect greater, cumulative physiological and genomic damage due to the extreme demands of territorial behaviour.
Student roles: The student will participate in all aspects of the project: 1. Fieldwork to identify and collect male carpenter bees. 2. Caring and maintaining live males in the lab. 3. Specimen preparation for RNASeq: freezing specimens in liquid nitrogen, evaluating body size and physical wear and tear, dissecting tissues, preparing RNA. 4. Cleanup, processing, and analyses of RNASeq data. Student will have the option of choosing specific biochemical pathways to focus their project on.
Skills required: Students should have taken courses in molecular biology. Experience in ecology, behaviour, evolutionary biology, and entomology would be beneficial. Enthusiasm for quantitative data analysis is crucial, as is willingness to learn programming in R and Python for bioinformatics.
47. Gene regulation and antigenic variation in Borrelia burgdorferi
Supervisor: Jenny Wachter
University: University of Saskatchewan (Saskatoon campus)
Our recent work has discovered added functions for a protein involved in the suppression of genes involved in vertebrate infection, including regulation of plasmid replication and antigenic variation. This project seeks to further define the roles and functions of this protein in pathogenesis.
Research area, student roles & skills
Research area: We are interested in gene regulation in pathogenic bacteria. Our lab uses molecular biology techniques to better characterize genes involved in virulence. Our research is organized around four main avenues: (1) Bacterial gene regulation that promotes and sustains infection in vertebrate hosts, (2) Pathogen-host and pathogen-vector interactions that enable bacterial survival and transmission, (3) Enhancing molecular research by creating new reagents and tools to enhance our understanding of understudied bacteria, and (4) Translational applications utilizing our discoveries to identify new therapeutics, vaccines, and antimicrobial strategies to combat infectious diseases and address the growing threat of AMR.
Student roles: The student will be expected to grow required bacterial stocks, perform biochemical assays, and cloning.To perform these experiments, the student will be expected to: Prepare various required media Maintain accurate daily records Interpret, evaluate, and discuss the results of each experiment with the supervisor as part of the planning process for studies. Write reports Maintain accurate daily records of experiments and results in pen in bound laboratory notebooks. Interpret, evaluate, and discuss the results of each experiment with the supervisor as part of the planning process for studies. Write reports of findings, stating methods and procedures, including modifications, specimens and materials involved, and results of experiments. Select the appropriate methodology and procedure to meet the experimental objectives. Make minor modifications of adaptions to established methods, procedures, and/or techniques to solve problems in the work. Assist in the analysis and interpretation of data and prepare summaries of laboratory findings to be used in reports and papers to be presented at scientific meetings and/or publications. Collaborate with scientists from other organizations or institutes involved with similar research projects.
Skills required: Knowledge in performing a wide variety of complex procedures and techniques which may include, but are not limited to: microbial cell culture and basic manipulations, proper aseptic technique, phage isolation and purification, phage plaque assays, PCR amplification of gene sequences, design of synthetic genes, gene cloning, bacterial transformations, gel electrophoresis, DNA analysis, DNA isolation, DNA purification, DNA sequencing, DNA gel purification, protein purification and analysis, western blots, PAGE, knowledge in presenting and interpreting data.
48. Genetic basis of traits
Supervisor: Malgorzata Gazda
University: Université de Montréal
Location: Montreal, Québec
Start date: 2027-05-03 (flexible)
Disciplines: Biological Sciences, Biology, Genetics, Computer Science, Molecular Biology, Veterinary Science and Medicine
To understand the genetic basis of traits (such as migration, song and coloration) is coded in the genome we will perform genomic scan comparing chosen breeds/species that have and don’t have a specific, selected trait. Blood samples will be collected and then the DNA will be extracted and sequenced with Illumina paired-end sequencing. Then the sequenced samples will be processed and followed with the genomics analysis. The sequencing reads will be mapped with bwa-mem to the species reference genome (eg. canary). To detect regions under selection across the genome, we will scan sequenced genomes for multiple patterns of molecular variation that can be detected comparing individuals from distinct groups: 1) local losses of heterozygosity; 2) locally elevated levels of genetic differentiation; 3) distortions in the allele frequency spectrum. To infer these signatures of selection in the genome, we will summarize levels and patterns of genetic diversity and differentiation applying the following statistics in a sliding window mode: population differentiation (FST), nucleotide diversity, sweepfinder and Tajima’s D, implemented in ANGSD. Moreover we will check the absolute difference in allele frequency (DAF) between different combinations of singing and non-singing canary breeds, calculated on base of snpcall wth Freebayes. The genome-wide screen will have the ability to identify multiple distinct or overlapping signatures of positive selection in breeds of interest not present in the breeds. Then, we will functionally annotate SNP and indel variants using the genetic variant annotation and effect prediction toolbox SnpEff. To search for causative mutations within candidate regions, we will screen these regions for variants that could potentially alter protein structure and function, such as nonsynonymous, frameshift, nonsense, and splice site mutations. Together, these analyses will generate a comprehensive list of candidate genes and mutations underlying specific trait, which will inform future functional experiments.
Research area, student roles & skills
Research area: In my research group we combine population genomics, classical genetics, biochemistry, physiology, molecular biology, phylogenetics, neurobiology and behavior. The interdisciplinarity is achieved thanks to wide range of collaborations as well as our willingness and courage to explore unknown. We work on wide variety of organisms from birds to plants, with main model species of canary birds. Other than genotype phenotype association we are also interested in understanding how gene function and expression evolve across species as well as how is it impacted by organismal biology, physiology and environment.
Student roles: Candidates depending on their experience and interest will be able to develop the bioinformatics skills, by managing the sequencing data, analysis whole genome sequencing data and comparing one phenotype with other, with basic population genetics statistics (eg. FST). Additionally there will be opportunity to learn practical skills in molecular biology lab, such as extracting DNA, preparing sequencing libraries, qPCR. For interested candidates potential to mix both, so the bioinformatics combined with the wet lab. Each intern will work on their own small project, that later will be implemented in the larger work. Each student will be expected to write a report based on their projects as well as present it at the group meeting.
Skills required: Candidates for the internship should be interested in evolutionary biology. Basics of scripting/basic programming will be beneficial but not required. Previous wet lab experience for a candidate in more laboratory experience will be beneficial. General understanding of evolutionary biology, genetics and genomics or computer science will be helpful in order to work in an interdisciplinary team with background in biology and computer science. Students should be willing to work collaboratively as a part of the team, with professionalism, attention to details and eager to learn.
49. Genomics-led drug discovery in parasitic nematodes
Parasitic worms, or helminths, infect more than 1.5 billion people and countless animals and plants globally. In humans,
long-term infections can lead to severe illnesses, particularly in impoverished and underserved communities, perpetuating
cycles of poverty. Parasitic worm infections in livestock and plants pose a threat to food security and result in significant
economic losses. Despite their importance to public health and the economy, we understand very little about parasitic
worm biology, particularly in how these worms evolved key life history traits, such as sexual differentiation, which are
essential to their survival and success.
Single-cell and single-nuclei RNA sequencing (sc/snRNA-seq) is a revolutionary technology that can identify cell types,
cellular pathways and molecular targets relevant to the development, growth and spread of parasitic worms. We are building
upon existing snRNA-seq data in a model helminth to generate the first complete cell atlas of a parasitic worm across
all life cycle stages and sexes. We are integrating this data with existing sc/snRNA-seq data from other helminth
species to create a pan-helminth cell atlas, providing a comprehensive understanding of gene expression across diverse
taxonomic levels. Integrating these data will help trace cell lineage development across the parasite lifecycle and elucidate
genes and cells involved in key conserved regulatory and metabolic pathways between evolutionarily distant helminths.
Research area, student roles & skills
Research area: The Buddenborg lab develops and utilize cutting-edge genomics tools to understand the developmental biology of parasitic worms that infect humans and animals. We combine expertise in computational and molecular biology to apply single-cell sequencing technologies for genome-led drug discovery.
Student roles: The student will assist lab members in smRNA-FISH experiments and/or computational analysis of single-nuclei RNA-seq data from parasitic nematodes. Depending upon prior experience, an independent project can be arranged.
Skills required: We are looking for students with an interest in either wet or dry lab techniques. The computational student would need to have existing basic knowledge of unix systems and basic coding experience. The wet lab student would need to understand the basics of RNA biology, RNAi techniques, and single molecule fluorescent in situ hybridization. Prior research experience is highly desired but not necessary.
50. Hepatocellular injury in Ebolavirus disease
Supervisor: Michael Hawkes
University: University of British Columbia (Vancouver campus)
Location: Vancouver, British Columbia
Start date: 2027-05-03 (flexible)
Disciplines: Biological Sciences, Medical Sciences, Medicine
Rationale and Background
Ebola virus disease (EVD) is a highly lethal infection characterized by multiorgan dysfunction. While hepatic involvement is recognized as a hallmark of filovirus pathogenesis, existing research heavily relies on static, single-time-point measurements at admission. The longitudinal progression of hepatocellular injury during the clinical course, and its independent contribution to patient mortality, remains poorly defined.
Objectives and Specific Aims
Using an established clinical dataset, this project aims to comprehensively describe the longitudinal trajectory of hepatic dysfunction during EVD among patients admitted to the Ebola Treatment Unit (2018-2020).
Our specific objectives are to:
-Quantify the prevalence and severity of acute liver injury at admission using aspartate aminotransferase (AST) and alanine aminotransferase (ALT) markers.
-Characterize the longitudinal trajectories of AST and ALT over the duration of hospitalization, comparing the kinetic patterns of fatal versus non-fatal cases.
-Determine the independent association between progressive hepatocellular injury and mortality using multivariable, time-varying survival models.
Methodology
We will conduct a retrospective cohort study analyzing ~340 patients admitted to two EVD treatment units over an eight-month period during the 2018–2020 outbreak in the Democratic Republic of the Congo. The dataset contains serial biochemical measurements, allowing for granular tracking of AST and ALT levels. We will utilize Cox proportional hazards models with liver enzymes treated as time-varying covariates, adjusting for baseline viral load and other critical prognostic factors to isolate the independent risk of hepatic failure.
Significance and Anticipated Impact
By defining the trajectories of liver injury that precede death, this study will provide critical insights into filoviral systemic disease. The findings will help clinicians identify high-risk patients earlier and provide a data-driven foundation for improved care in the Ebola Treatment Unit.
Research area, student roles & skills
Research area: I lead a research program in global pediatric infections. Diseases of focus include some of the leading infectious disease killers of children in the world today: Ebola virus disease, malaria, and pneumonia. Study methods include translational science, epidemiologic studies, and clinical trials. I operate field sites in Uganda and the DRCongo. His research is improving outcomes in children with serious infections in some of the least developed areas of the globe. This program of research is anchored in a philosophy of global interdependence and ethical duty toward the world’s least advantaged children.
Student roles: In this research project, the student will analyze quantitative field data to evaluate risk factors and assess a critical research question: What is longitudinal course of AST and ALT in Ebola virus survivors and fatalities? The ultimate objective is to complete a research manuscript for peer-reviewed publication by the end of the summer. Through this hands-on experience, the student will develop core competencies in clinical data processing, modeling, and visualization. By evaluating these datasets, the student will learn to identify statistical associations, critically assess cause-and-effect relationships, and synthesize complex findings for a medical audience. This role offers a unique opportunity to engage directly with researchers and CHWs in the DRC, providing valuable exposure to global health settings, diverse cultural contexts, and international healthcare systems. Finally, the project will significantly strengthen the student's scientific writing and communication skills. By taking a lead role in drafting the manuscript, the student will master the structure of academic papers, learn to translate raw statistical results into clear narrative conclusions, and develop the ability to defend data-driven interpretations in a direct, accessible, and scientifically rigorous manner.
Skills required: Interest in infectious diseases, clinical medicine, or public health. Because the data from the 340 patients have already been collected, this role focuses heavily on scientific literature review, data interpretation, and manuscript drafting. The ideal candidate does not need advanced statistical skills but should possess excellent scientific writing abilities and a keen interest in learning how clinical biomarkers relate to patient outcomes. Guidance will be provided for the statistical analysis, making this an ideal project for a student looking to develop their medical writing skills and gain experience in longitudinal analysis.
51. Horizontal gene transfers in symbiotic fungal organisms
Supervisor: Yan Wang
University: University of Toronto (Scarborough campus)
Horizontal Gene Transfer (HGT) is a process in which organisms acquire foreign genes from different species. HGT contributes to organismal evolution and has been an important source of gene c diversity. HGT was commonly identified in prokaryotes but rarely reported in eukaryotes. However, our understanding of HGT in eukaryotes is rapidly expanding with the production of genomic resources and the development of detection tools. The Kingdom Fungi represents a striking example, especially the ones known as obligate symbionts, which interact intimately with various host organisms. Our research group has been dedicated to detecti ng fungus-related HGT elements and has discovered several such cases in fungal species including the mosquito gut-dwelling fungi (doi:10.1093/molbev/msw126), herbivorous mammal rumen fungi (doi:10.1128/mSystems.00247-19), amphibian gastrointestinal fungi (doi:10.1534/g3.120.401516), and photobiont-associated fungi (doi:10.1016/j.cub.2021.01.058). This project aims to identify novel HGT using newly assembled fungal genomes from the lab, representing underexplored lineages on the Tree of Life. The student working on this project will help refine lab's existing pipelines and analyze the fungal genomes as well as related host data to reconstruct the evolutionary history of identified genes by conducting comparative genomics. A high-impact research report will be accomplished and aimed for publication at the end of the project.
Research area, student roles & skills
Research area: Dr. Yan Wang is interested in fungal genomics and evolution using whole-genome scale data across the Kingdom Fungi. Part of the Wang Lab efforts is to create and analyze new data at the -Omics levels, including genomics, transcriptomics, metabolomics, and proteomics, aiming to find important evolutionary changes and adaptation strategies (e.g., whole-genome duplica on, inter-species hybridiza on, horizontal gene transfer, noncanonical gene c codon usage) employed by microbial fungi to fulfill their lifecycle and in interac ons with various hosts, such as animals, plants, pro sts, etc.
Student roles: The student working on this project will help identify HGT candidates in newly assembled fungal genomes using lab pipelines and tools. The student will be able to refine the tools and improve the overall efficiency. The student will be able to learn from the experts and collaborate with multiple internationally renowned scientists. A high-impact research report will be aimed for publication at the end of the project.
Skills required: Minimum requirements: Basic programming skills in Linux, Python, and R; effective communication skills Preferred qualifications: strong interests in comparative genomics, data visualization, and competencies in writing and public speaking.
52. Human Disturbance and Biodiversity Change in Alpine Ecosystems: A Longitudinal, Multi-Methods Study of Recreational Impacts in Nch’kay (Garibaldi) Provincial Park
Supervisor: Nina Hewitt
University: University of British Columbia (Vancouver campus)
One position is available working with a dynamic group of ecologists in UBC Geography and the Biodiversity Research Centre to assist in a study of human impacts on plant biodiversity in the alpine zone of Garibaldi Provincial Park (around Nch’Kay mountain). This project is part of cross-university research to study the effects of climate change and human disturbance on alpine tundra ecosystems. Fieldwork focuses on plant biodiversity and environmental monitoring using quadrat sampling and sensors to document impacts on this fragile ecosystem.
Since 2022, UBC Geography has led a long-term alpine monitoring program examining recreational trampling along heavily visited routes in Garibaldi Park, including Taylor Meadows, Black Tusk, and Panorama Ridge. We established long-term transects in trail-adjacent and control plots, conducted biodiversity assessments, and initiated drone surveys. We have measured community composition, plant cover, and phenological traits of focal alpine species. Mosses and graminoids are identified to species level with expert support, enhancing biodiversity resolution. More recently, we integrated drone imagery and spectral analysis to detect vegetation change, supporting plant community classification and year-over-year monitoring.
In 2027, we will expand this work with gradient-based trampling measurements, plant trait assessments, and environmental monitoring. We will also collect repeated drone imagery across the study area to track change over time. Our approach integrates field plots, remote sensing, and species-level identification to support long-term alpine ecosystem monitoring and help Park managers track ecological impacts of rising visitation, climate change, and invasive species.
The student researcher will participate in field data collection in Taylor Meadows and other local field sites; enter and help analyze community composition data; and work with environmental datasets (TOMST instruments and wildlife cameras) with support from our team. They will also use visualization tools to communicate fieldwork results and contribute to public outreach.
Research area, student roles & skills
Research area: I specialize in disturbance ecology, plant colonization and invasion, and biodiversity responses to climate change. I am co-PI of the Alpine Horizons Research Initiative, which aims to better understand and steward British Columbia’s alpine ecosystems in the south Coast Mountains. Drawing on more than a decade of research in temperate forest, Arctic, and alpine systems, I have published in leading journals (e.g., Biological Conservation, Forest Ecology & Management) and was an invited contributor to the Canadian Mountain Assessment, a peer-reviewed synthesis of Western and Indigenous knowledge. I also develop and evaluate virtual and augmented reality experiences for communicating bio-geoscience.
Student roles: The successful candidate will assist with pre-trip planning (packing equipment, attending meetings), data collection in the field (hiking into and camping at Taylor Meadows campground to conduct field work including plant surveys, environmental sensor installation; possible drone image collection), and post-trip data processing (data entry, quality checking, data management and analysis if trained). With direct supervision from the project manager, the candidate will learn to complete field research, coordinate tasks with the field team, as well as develop basic computational skills for data management. Candidates from ecology/biogeography/environment backgrounds and experiences are encouraged to apply, and must demonstrate a strong motivation to learn how to safely and efficiently conduct field work in teams.
Duties may partially change, but will likely include some of the following : Pre-fieldwork • Attending and organizing pre-trip meetings (~1-3x month from June-August) • Coordinate communication among the project team regarding field dates and campsite arrangements • Preparing for multi-day camping trips Fieldwork • Hiking into and camping in Taylor meadows and nearby site(s) for 4-5 x 3 to 5-day trips in Jun, Jul, Aug • Setting up transect studies, climate stations, and cameras early in the summer (during a 3-4 day trip in June) • Learning plant species identification • Measuring, scoring and evaluating of plant species and traits (specific leaf area, number of leaves, etc.) • Working with supervisor to sample tree rings from meadow populations for age-size classification • Potentially: Collecting remotely sensed imagery, either in lab or field (drone imagery) for pixel classification Post-trip • Organizing and backing up of data in spreadsheet • Meeting to discuss post project survey/feedback, assessment of goals met, skills acquired. • There may be work with satellite and/or drone imagery for pixel classification if the student is qualified
Skills required: The student should have a background in a discipline related to ecology/biology/environmental science. Experience working with environmental and geospatial data (GIS/geomatics) and/or remotely sensed data is an asset but not required. Training will be provided during the course of the position, but preference will be given to students who have experience in, and are highly motivated to conduct remote field work in alpine settings (e.g., European Alps, North American alpine systems). Experience working in the alpine, camping, and working with plants is desirable. We encourage students with reasonable level of experience in plant ecology or lab data analysis to apply.
53. Identification of early Alzheimer's Disease
Supervisor: Sabine Mai
University: University of Manitoba (Winnipeg campus)
Telomeres, the ends of chromosomes, shorten with each cell division. A meta-analysis of studies that examined telomeres of 860 Alzheimer’s Disease (AD) and 2022 control subjects found that telomeres are shorter in brain tissues and in tissues other than brain, including peripheral mononucleated cells, leukocytes, lymphocytes, monocytes, and buccal cells.
Telomere length is not the only feature of telomere dysfunction. We therefore developed a three-dimensional (3D) analysis tool for telomeres that examines the 3D spatial organization of telomeres as well as their length and numbers, their aggregation, positioning and cell cycle distribution with single cell precision and a quantitative software.
In our work related to AD, we have collected cheek swabs from AD and controls and performed 3D quantitative fluorescent in situ hybridization (3D Q-FISH) on buccal cells using a peptide nucleic acid (PNA)-telomere probe (Cy3 labeled). We have shown that the 3D spatial telomere organization as well as telomere length and numbers are significantly different in patients with AD compared to cognitively healthy individuals. Of specific interest to this application are our recent data on mild AD; in this work, we have for the first time evaluated the 3D telomere biomarker in early AD and confirmed the findings with amyloid PET. We were able to clearly distinguish cognitively healthy and ad study participants.
It is our goal to validate and establish 3D telomere screening of buccal cells as an early indicator of mild AD that can then be followed up by additional test modalities. Student(s) who work on this project will validate 3D telomere profiles of buccal cells in an independent cohort.
Research area, student roles & skills
Research area: Nuclear architecture in disease (cancer and Alzheimer's Disease), 3D imaging, super resolution imaging, fluorescent in situ hybridization, quantitative imaging and analysis, biomarkers of disease
Student roles: - carry our research - summarize data - present data - participate in lab meetings
Skills required: - able to perform lab work with precision - able to document daily lab activities - able to conduct SOPs after training - able to present data and work in a team
54. Identifying Plant Genes that Promote Healthy Microbiomes and Plant Health
Plants host diverse microbial communities that contribute to growth, nutrient acquisition, stress tolerance, and disease resistance. Maintaining a balanced microbiome is therefore essential for plant health. However, the genetic mechanisms that plants use to regulate these microbial communities remain poorly understood. This project aims to identify and characterize plant genes that promote healthy microbiomes and prevent microbiome imbalance.
Previous work in my laboratory used a forward genetic screen in the model plant, Arabidopsis thaliana, to identify mutants with disrupted plant-microbiome interactions. Several mutants, termed "guardians of normal microbiota" (grm), were isolated. Characterization of the first mutant, grm1, revealed a previously unknown mechanism involved in maintaining microbiome homeostasis and demonstrated the power of this genetic resource for discovering new regulators of plant-microbe interactions. The remaining grm mutants likely represent additional components of the genetic network that promotes healthy microbiomes.
The project will focus on characterizing these mutants through phenotypic analysis, microbiome profiling, and genetic approaches. Students will assess plant growth and development, quantify microbial populations associated with each mutant, and perform genetic crosses to determine relationships among mutant lines. Selected mutants will be used to generate mapping populations that will enable identification of the underlying genes responsible for the observed microbiome defects. Students will gain hands-on experience in plant genetics, microbiology, molecular biology, experimental design, data analysis, and scientific communication.
This research will generate foundational knowledge of the host genetic mechanisms that support beneficial plant-microbe interactions. The results will provide new targets for future mechanistic studies and may ultimately contribute to strategies for improving crop productivity and resilience through microbiome-informed approaches.
Research area, student roles & skills
Research area: My research focuses on understanding how plants maintain healthy relationships with the microbial communities that live on and within them, collectively known as the plant microbiome. These microbes influence plant growth, nutrient uptake, stress tolerance, and disease resistance. Using the model plant Arabidopsis thaliana, my laboratory combines genetics, microbiology, molecular biology, genomics, and bioinformatics to identify plant genes that regulate microbiome composition and function. The long-term goal is to uncover mechanisms that promote beneficial plant–microbe interactions and support plant health, providing knowledge that may contribute to more resilient and sustainable agricultural systems.
Student roles: The student will contribute to an ongoing research project aimed at identifying plant genes that promote healthy microbiomes and support plant health. Working under the supervision of the principal investigator and alongside other laboratory members, the student will participate in a range of research activities related to plant genetics, microbiology, and molecular biology.
Specific responsibilities may include plant cultivation and maintenance, phenotypic characterization of Arabidopsis mutant lines, microbiota sampling and quantification, genetic crosses, DNA extraction, preparation of samples for sequencing, data collection, and basic data analysis. Depending on project progress and the student’s interests and abilities, additional opportunities may include molecular cloning, genotyping, and bioinformatics analyses.
The student will be expected to maintain accurate laboratory records, follow experimental protocols and safety procedures, participate in regular meetings, and communicate research progress with supervisors and team members. They will be encouraged to contribute ideas, ask questions, and develop increasing independence in experimental design and problem solving as the project progresses.
Throughout the placement, the student will receive training and mentorship in laboratory techniques, data interpretation, scientific communication, and responsible conduct of research. By the end of the project, the student is expected to have developed practical research skills, gained experience working in a collaborative research environment, and contributed to generating new knowledge about the genetic mechanisms that regulate plant-microbiome interactions.
Skills required: This project is open to undergraduate students with an interest in plant biology, microbiology, genetics, or related fields. Previous research experience is an asset but is not required. The most important qualifications are reliability, responsibility, attention to detail, and a willingness to learn. Students should be able to follow protocols carefully, maintain accurate records, and communicate effectively with supervisors and team members. Successful applicants will demonstrate curiosity, initiative, and a positive attitude toward problem solving. Training will be provided in all necessary laboratory and analytical techniques, allowing students to develop research, critical thinking, and scientific communication skills.
55. Immune Cell Plasticity Using Stimuli‑Driven Reprogramming Models
This project investigates how immune cells can be reprogrammed into altered functional states when exposed to defined cytokines, chemical stimuli, or co‑culture environments. The student will perform in‑vitro cell culture, maintain immune cell lines or primary cells, and expose them to controlled stimuli (e.g., IFN‑γ, IL‑4, LPS, metabolic modulators). They will then assess reprogramming outcomes using hands‑on functional assays, including cytokine‑release assays (ELISA), phagocytosis or cytotoxicity assays, ROS production, nitric oxide quantification, and viability/proliferation measurements. The student will also perform co‑culture experiments, pairing immune cells with target cells (e.g., cancer cells, epithelial cells, or stromal cells) to observe how cell–cell interactions influence immune plasticity. Data will be analyzed to determine how different stimuli shift immune cells toward activated, suppressed, or alternative phenotypes.
Research area, student roles & skills
Research area: Our lab focuses on understanding immune responses in cancer and discovering biomarkers for early disease detection. Our interdisciplinary research integrates in vitro diagnostics, spectroscopy, and machine learning to investigate the metabolic reprogramming of immune cells within the tumor microenvironment, with applications spanning both cancer and oral health. The goal is to bridge the gap between fundamental scientific discoveries and clinical applications, particularly in the area of early disease detection.
Student roles: The student will perform experiments related to immune‑cell reprogramming. This includes maintaining immune‑cell cultures, applying defined cytokine, metabolic, and establishing co‑culture systems with target or stromal cells to model microenvironmental interactions. They will perform functional assays, including cytokine‑release assays (ELISA), phagocytosis or cytotoxicity assays, viability/proliferation assays, Seahorse extracellular flux analysis to quantify metabolic reprogramming (OCR/ECAR), raman data colelction, Dna/Rna extraction. The student will be responsible for optimizing assay conditions, troubleshooting experimental variability, and ensuring high‑quality data collection. The student will analyze results using appropriate software, generate publication‑quality figures, and contribute to scientific interpretation of immune‑cell state transitions. They will prepare structured experimental summaries, assist in drafting manuscript‑style sections (methods, results, figure legends). The student will participate in regular discussions to refine hypotheses, compare experimental conditions, and propose follow‑up assays. Overall, they will play an active role in both the experimental and scientific‑communication components of the project. Students from any background who feel they can meaningfully contribute are welcome to apply.
Skills required: The student should have prior experience with mammalian cell culture, including aseptic technique, media preparation, and maintaining suspension or adherent cell lines. Familiarity with basic immunology, cytokines, and cell‑state transitions is helpful. Experience performing functional assays such as ELISA, ROS/NO detection, viability assays, or cytotoxicity/phagocytosis assays is strongly preferred. The student should be comfortable following detailed protocols, handling biological samples, and recording experimental data. Basic data‑analysis skills using Excel, GraphPad, or Python/R are an asset. Prior exposure to co‑culture systems, flow cytometry, or microscopy is beneficial but not required.
56. Impact of IFN-γ and infection on the microbiome
Previous research from the Pardy lab has shown that IFN-γ signalling to intestinal epithelial cells (IEC) is crucial for optimal control of infection with Cryptosporidium parasites. Experiments in cell culture indicate the same, as incubating IEC grown as organoids with IFN-γ prior to infection limits parasite replication. However, the magnitude of the impact IFN-γ has in culture is much more limited relative to its importance in mice. This suggests that while IFN-γ acts directly on IEC, IEC may control infection both by directly attacking the parasite and by other, indirect effects.
In addition to participating in host defence, IEC interact constantly with the intestinal microbiota. Previous research has shown that IFN-γ induces antimicrobial peptide production by IEC and changes to the microbiome. Cryptosporidium also causes major shifts in intestinal microbial communities, however the extent to which these are due to infection or IFN-γ is unknown. Intriguingly, secondary metabolites produced by the microbiota have important anti-parasitic effects. Thus, this project will test the hypothesis that IFN-γ signalling to IEC alters the microbiome to make the intestine more hostile to Cryptosporidium infection.
Research area, student roles & skills
Research area: The Pardy lab studies interactions between cytokines called interferons and intestinal epithelial cells (IEC). These cells form the barrier between the contents of the intestine and the rest of the body. However, they are often the target of infection for many orally infectious pathogens. Determining how IEC respond to interferons to defend themselves could be useful to improve how we treat gastrointestinal infections while limiting inflammatory side effects.
Student roles: Conduct experiments and data analysis, have weekly meetings with the supervisor, analyze data, and present twice in lab meeting (once at start and end of internship).
Skills required: Knowledge of immunology and familiarity with related lab techniques. Familiarity with R Studio/sequencing techniques (e.g. 16S) and analysis is an asset.
57. Impacts of livestock grazing on plant biodiversity in endangered Alvar ecosystems
The project involves field sampling of vegetation communities located within the Carden alvar.
Three field surveys will be completed during the growing season (spring, mid summer, and late summer) to ensure that we capture as much of the diversity as possible, given that plants have seasonal variation in timing of emergence, flowering periods, and abundance that would likely be missed if only completing a single survey. Species diversity will be quantified using a systematic approach. A series of nested quadrats along transects will be established to estimate plant species richness, abundance and % cover for trees, shrubs, and herbaceous plants in each of the nine sites.
Estimates of species diversity (Simpson’s Diversity Index (D), richness, evenness, dominance) and the proportion of exotic species will be compared and contrasted between meadow alvar communities experiencing heavy, moderate, or no livestock grazing while accounting for factors such as site history, intentional introduction of non-native forage plants, presence/absence of ungulate herbivory, etc. This will be done using a mixed-model approach given that we will include fixed and random effects; nb., site and management factors such as the timing and extent of grazing, intentional introductions of non-native forage plants such as clovers and grasses, herd size, evidence of deer and moose browsing, soil properties, presence of invasive plant species, nutrient cycling, etc.
Research area, student roles & skills
Research area: Alvars are naturally-occurring dry grasslands established on thin, discontinuous soils overtopping limestone plains. They are special, globally-rare ecosystems that support a variety of rare, endemic, and threatened species that are not found anywhere else, and thus, are important sites for conservation. An important threat to alvar ecosystems is livestock grazing. The purpose of our study is to examine how livestock grazing intensity (non-grazed, moderately grazed, heavily-grazed) impacts native, endemic plant communities in the Carden Alvar located in Ontario, Canada.
Student roles: The students will assist my graduate students with the field work described above. There is also opportunity to contribute with the writing of manuscripts & be included as an author in a peer-reviewed publication. An example of my past Mitacs students contributing to a publication can be viewed here: https://www.tandfonline.com/doi/full/10.1080/03055698.2024.2405809 Four of the authors are former Mitacs student interns Students will also help with periodic outreach events that help to inform the public about the importance of alvar communities.
Skills required: Students who have some field experience are preferred, but this is not a requirement. We are mostly looking for enthusiastic students with a keen interest in biological conservation. We will be providing training on plant identification as well as community & landscape ecology. A statistics background is also desirable, but again, not a requirement as we will provide training on the techniques needed to complete our study.
58. Investigating hrp2 expression accross Plasmodium life cycle
Supervisor: Claire Kamaliddin
University: University of Alberta (Edmonton campus)
The project focuses on the hrp2/3 genes of Plasmodium falciparum, the causal agent of human malaria. These proteins are unique to P. falciparum and are often used as diagnostic targets in rapid tests; however, an increasing number of parasites carry deletions of these genes.
HRP2 and HRP3 are two proteins unique to P. falciparum, and the genes hrp2 and hrp3, both containing two exons, are located on the labile subtelomeric regions of chromosome 8 and 13, respectively23. HRP2/3 present high content in histidin, particularly in the low complexity regions of the protein (coded by exon 2), which differ from most protein sequences. HRP2/3’s primary structure is of low complexity, which suggests unconventional secondary and tertiary structure. Studies have shown their expression at high level during the IDC, particularly in ring-stage forms. Nonetheless, HRP2/3’s function is not fully elucidated, and there are no homologs in other species that could inform its role
In this project, we investigate how hrp2/3 proteins are expressed and produced across the Plasmodium life cycle. We use in vitro models to collect RNA and protein extracts from several P. falciparum laboratory strains grown in the lab, and assess the timing of intracellular development using light microscopy. The proteotranscriptomic assessment will be performed both according to our published methodology and using the newly developed proteomic method (development ongoing in the lab). In addition, inhibitors of hemozoin formation, such as chloroquine and quinine, will be used to investigate by-pass mechanisms both in the presence and absence of HRP2/3 across the IDC.
We will analyze the IDC and its proteotranscriptomics dynamics between strains presenting hrp2/3 deletion, their complemented counterparts, and the control strains. We will compare the timing of the IDC, P. falciparum’s growth rate, and protein expression using differential protein abundance analysis (as performed in our previously published work).
Research area, student roles & skills
Research area: I am a translational scientist, who uses a blend of in vitro models and clinical studies to decipher host pathogens dynamics that contribute to infection clearance and resistant/tolerant phenotypes in eukaryotic pathogens (parasites and fungi).
Student roles: The student will specifically conduct the protein quantification, and RT-qPCR (RNA expression) necessary for this project. They will independantly analyse the data and be trained and mentored in analysing their experimental results. Depending on the project's progress, the student will also be involved in RNA sequencing experiments design and implementation.
Skills required: Experience in wet lab methods (PCR, qPCR, western blot, electrophoresis, microscopy), ability to work in aseptic conditions. Organized, best laboratory practices, and scientific integrity are paramount. Any health or biological sciences (including medicine, pharmacy programs) is preferrable. The student must be able to work in a team environment, interact respectfully with their peers, and work collegially.
59. Isolation and characterization of new bacteriophages from environmental samples
Infectious diseases are among the leading causes of death, and plant pathogens cause large crop and economic losses worldwide. Antimicrobial materials are a key strategy to counteract these alarming trends in health and food safety.
Bacteriophages (phages)—bacteria-infecting viruses—have recently re-emerged as an appealing approach to bacteria control. Additionally, genetic engineering is a potential strategy to rapidly tailor phage properties for new technologies.
By combining phages with appropriately designed materials and genetic modifications, our research aims to use engineered phages in treatments and biocontrol agents against human and plant bacterial diseases. In order to develop these technologies, it is of primary importance to establish a phage isolation and characterization program to expand the repertoire of available candidates.
The overall objective of the project is the isolation and characterization of new phages from environmental samples. This project will continue the efforts of a program of discovery of new microorganisms recently established in Professor Rubino's research group. The first part of the project will be conducted outdoors for the collection of environmental samples. Then, the majority of the research work will be in the lab, characterizing the phages isolated by the student and during previous phage isolation work. All necessary technical training on the microbiology and molecular biology techniques will be provided. Finally, a part of the internship will consist of data analysis and presentation of the results.
The outcome of this project will contribute to the development of infection prevention and pathogen control strategies by helping to build the repertoire of well-characterized phages that can be employed for innovative technologies for treatments and biocontrol agents.
Research area, student roles & skills
Research area: This project deals with the discovery of new bacteriophages (phages in short, i.e. viruses that infect bacteria but not humans), for the development of technologies for therapies, detection and prevention of bacterial diseases. The project will be conducted under the supervision of Professor Ilaria Rubino, who specializes in the fields of phages, phage engineering, and antimicrobial materials. She focuses her research themes on the synergy between materials, biological systems and infection prevention, in order to develop technologies that aim to improve global health and pathogen control.
Student roles: In order to discover phages, the project will include: 1) Collecting environmental samples (from soil, lakes, rivers, crop fields, etc.); 2) Culturing of the targeted bacteria; 3) Isolation and purification of phages contained in the environmental samples; 4) Phage amplification; 5) DNA extraction from the phages for sequencing; 6) Characterization of the phage virulence against an array of bacteria; 7) Visualization of the phages by transmission electron microscopy (TEM); 8) Naming and archiving the new phages; 9) Analysis, interpretation and summary of the results. The project will be mainly carried out in the laboratory of Professor Rubino as well as in the laboratories of the Department of Chemical and Biotechnological Engineering, located at the Faculty of Engineering of the Université de Sherbrooke. The work environment in Professor Rubino’s group aims to provide opportunities for initiation and consolidation of the students’ research skills. The interpretations and ideas of all group members are taken into consideration and greatly encouraged. The student will be invited to share their questions and results during weekly meetings. The student will have the opportunity to interact with the technicians of the Department of Chemical and Biotechnological Engineering, as well as with collaborators in the Faculty of Medicine and the Department of Biology with whom Professor Rubino collaborates for phage isolation efforts. The student will have access to a desk and all necessary equipment. The student will plan their research work, ensuring a flexible work schedule; if needed, the dates of beginning and end of the internship could be modified. The student will be offered the opportunity to present at student symposia.The results could lead to a scientific publication.
Skills required: Introductory knowledge on the following topics would be an asset, but not a requirement: (i) Microbiology: bacteria cultures, phages; (ii) Molecular biology: isolation, digestion and sequencing of DNA (iii) Preparation of TEM samples and image analysis
60. Landscape Drivers of Boreal Lake Food Webs: Implications of Oil Sands Development
Freshwater lakes are closely connected to the landscapes around them. Water, nutrients, organic matter, and other materials move from surrounding watersheds into lakes, influencing water chemistry, habitat conditions, primary producers, primary consumers, and broader food-web dynamics. Understanding these landscape–lake linkages is essential for predicting how freshwater ecosystems respond to environmental change, especially in regions experiencing intensive resource development.
Northern Alberta, including the Athabasca Oil Sands Region, provides an important setting for examining how industrial development may influence boreal lake ecosystems. Oil sands activities may affect freshwater systems through direct pathways, such as accidental spills or tailings pond seepage, as well as through indirect pathways associated with landscape modification. Roads, pipelines, wells, mines, vegetation clearing, and other land-use changes can alter hydrology, sediment movement, nutrient delivery, and habitat structure. Atmospheric deposition of acidifying and eutrophying compounds may also influence lake conditions and biological communities.
This project will examine how watershed-scale land cover and land use are related to phytoplankton and zooplankton communities in 60 northern Alberta lakes adjacent to the Athabasca Oil Sands Region. Existing datasets include phytoplankton and zooplankton abundance and biomass, along with lake physicochemical variables. Corresponding landscape variables will be derived from geospatial databases available through the Alberta Biodiversity Monitoring Institute.
The student will help explore how landscape characteristics are linked to lake environmental conditions and biological responses, including phytoplankton and zooplankton community composition and biomass. Using R and GIS-based approaches, the project will combine ecological monitoring data with spatial landscape information to identify patterns across lakes. This research will provide valuable baseline information for assessing current and future impacts of environmental change and industrial development on boreal lake food webs. The project will also offer training in freshwater ecology, landscape ecology, spatial analysis, and quantitative ecological modelling.
Research area, student roles & skills
Research area: My research integrates aquatic ecology and quantitative ecology, with a focus on understanding and predicting the cumulative effects of human activities on freshwater ecosystems. I use quantitative approaches to explore ecological patterns and processes across multiple spatial scales, from local to continental, and across a range of organisms, from microbes to fish. I am committed to advancing data-driven, policy-relevant science to inform sustainable freshwater resource management.
Student roles: The student will contribute to data preparation, spatial analysis, statistical modelling, and interpretation. They will compile and organize existing lake physicochemical, phytoplankton, and zooplankton datasets, and assist with delineating watershed boundaries and extracting land cover and land-use variables using GIS and geospatial databases.
A major component of the role involves learning and applying advanced statistical methods, such as multivariate analysis and structural equation modeling (SEM), to examine how landscape factors influence aquatic communities and/or food-web dynamics. The student will use R for data analysis and gain valuable experience working with large ecological datasets.
A major component of the role involves learning and applying advanced statistical methods, such as multivariate analysis and structural equation modeling (SEM), to examine how landscape factors influence aquatic communities and/or food-web dynamics. The student will use R for data analysis and gain valuable experience working with large ecological datasets.
As a supervisor, I am committed to providing a supportive and collaborative learning environment. I will offer regular one-on-one mentorship, guiding the student through all stages of the project from data compilation and analysis to interpretation and scientific writing. The student will also have opportunities to engage with my broader research network and contribute to publications resulting from the project.
Skills required: The ideal student should have a background in ecology, environmental science, biology, statistics or a related field, with an interest in aquatic ecosystems and quantitative modeling. Strong analytical skills and experience with statistical analysis (preferably in R) are desirable. Familiarity with GIS is an asset but not required. The student should be comfortable working with large datasets and have strong written and verbal communication skills. Curiosity, initiative, and the ability to work both independently and collaboratively are essential for success in this project.
61. Light, current, action! Photoelectrochemical degradation of contaminants of emerging concern
A major stream of our research involves developing methods for targeted degradation of contaminants of emerging concern (PFAS, disinfection byproducts, pharmaceuticals, fertilizers, pesticides, etc.) in water. Using a photochemical and photoelectrochemical systems we are targeting understanding the mechanistic underpinnings of these processes, as well as practical scale-up for use in water treatment contexts.
Research area, student roles & skills
Research area: The Green Safe Water Lab, run by Professor Heather Buckley, is associated with IESVic and CAMTEC at the University of Victoria. We are committed to providing safe drinking water to communities in a sustainable, energy efficient manner. We span the interface between chemistry and civil engineering, with a dose of life cycle thinking and public health.
Student roles: The Research Assistant will work closely with Prof. Buckley and graduate students in the Green Safe Water Lab to prepare new photoactive materials and develop assays for monitoring degradation of contaminants of emerging concern by mass spectrometry and other methods. This will include: synthesis, characterization, spectroscopy, and data analysis to determine impacts of different conditions on degradation pathways. The Research Assistant will be trained in all of these techniques, and will be expected to keep careful records of their work, and will have opportunities to present their research within the team and in the broader university community.
Skills required: Introductory laboratory experience, intro chemistry. Experience in a "wet lab", and with project design and management is an asset. Experience with synthesis and spectroscopy are assets but not required. Experience working with surface chemistry and materials is a bonus! The student is expected to be responsible, professional, and communicate clearly and promptly with members of our team. We are a diverse group and we are thrilled to have international students joining us!
62. Looking at response to treatment in prostate cancer at an unprecedented resolution.
Rationale. Prostate cancer is a complex disease where gene regulation plays a crucial role during the tumor development and progression. Central to progression of the disease is the androgen receptor (AR), a ligand-inducible transcription factor controlled by androgens. Accordingly, antiandrogen therapies are very efficient in the early stages of the disease. However, resistance to treatment will inevitably arise. Adding to the complexity, the prostate tissue is heterogeneous, with multiple cell types involved in cancer progression and response to treatment creating diagnostic, stratification, and therapeutic challenges. To reach precision oncology, we will need to determine how specific antiandrogen treatments impact the transcriptional program of each cell.
Objectives. The overarching goal of the project will be to determine which cells are sensitive to antiandrogen treatment and why. Our central hypothesis is that the transcriptional program driven by the AR determines the sensitivity to antiandrogen therapies in prostate cancer. We will tackle 2 objectives:
Objective 1 – Study the response to androgens at the single cell level. We will investigate the transcriptional mechanisms controlled by the AR in individual prostate cells using single-cell technologies. In the process, we will compare the biomedical value of organoids and tumor explants to study cellular heterogeneity.
Objective 2 – Define the cellular mechanisms leading to resistance to antiandrogen. Using samples from patients resistant and not to antiandrogen treatments, we will characterize the single-cell response. We will develop organoids model to recapitulate the resistance phenotypes and explore new treatment avenues.
Outcomes and significance. With this project, we will provide an in-depth understanding of the AR-driven gene expression program at an unprecedented resolution. We will molecularly define the regulatory networks associated with cells responsive, but also resistant to antiandrogen treatment. These discoveries will be used to optimize transcriptional interventions in prostate cancer.
Research area, student roles & skills
Research area: Gene regulation plays a crucial role in the development, progression and treatment of diseases. Central to these mechanisms is the role of transcription factors which dictate the gene expression program. In cancer, aberrant regulation of transcription factors is often associated with gene programs promoting cell proliferation, survival, angiogenesis, and metastasis. Accordingly, multiple therapeutic approaches aim at controlling transcription factors. However, while efficient at first, tumors will develop resistance mechanisms to circumvent the therapeutic pressure. We are studying how the transcriptional program of cancer cells evolve under treatment to uncover new therapeutic approaches to optimize transcriptional interventions.
Student roles: Applicants will join the laboratory of Dr Steve Bilodeau located at the Centre de Recherche du CHU de Québec – Université Laval. Selected students will be joining ongoing projects with established protocols and procedures. Senior research assistants and students will provide daily supervision and training to execute the experiments and analyses. Students will be expected to gradually reach independence and the ability to design and conduct experiments before the end of their internship.
Skills required: Candidates from the entire spectrum of biomedical research are welcomed (computational, experimental, and clinical). In addition, candidates from other research fields (informatics, mathematics, engineering, etc.) are welcome to apply if they can clearly explain their rationale to pursue a career in biomedical research. Candidates will be selected based on their accomplishments and research experience.
Our lab is involved in a reserch project in which we quantify the monkey behaviour after long term exposure to certain drugs. Monkeys live in community of 6 to 8 individuals. During the study we have to identify each of them and manually score their behaviors. This is a very labour intensive process. So first, we aim at finding a facial recognition to identify individual monkey. Second, we are looking to have a supervised learning model to do the behavior scoring.
Research area, student roles & skills
Research area: Our neuroscience laboratory is working with monkeys to asses the long term effect of different drugs. Our lab has built different tests to measure monkeys and rats behaviour.
Student roles: We acquire large amount of video feed from monkeys that have been manually scored by human observer. The trainee can propose different model and test them to find the best model to match the human observer.
Skills required: Bash (needed) since we are using HPC, we are using super computer cluster. Mostly Python programming in VSCode and Jupyter. Then some basis in openCV, Yolo, Tensorflow, Pytorch, Keras, Sciklit (not necessairly all of them). Basic SQL to acces the database.
The research project will involve the creation of new machine learning or other analytical systems to work with genomic and epigenomic data, including data from ChIP-seq, ATAC-seq, CUT&RUN, Hi-C, cfMeDIP-seq, and other genomic assays.
Research area, student roles & skills
Research area: We develop machine learning techniques to better understand chromatin biology. These models and algorithms transform high-dimensional functional genomics data into interpetable patterns and lead to new biological insight.
Student roles: You will help design and test the new system, potentially prepare it for distribution to the public, and draft a manuscript to submitted to a journal on its operation.
Skills required: Required: - experience with Python or R - coursework in computer science, electrical engineering, statistics, or similar areas and interest in biology OR coursework in biology and interest in computational science
Preferred but not required: - coursework in statistics - experience with Unix and shell scripts - experience with NumPy, Pandas, and Seaborn
65. Mapping ecosystem services associated with fishes across biomes
Functional diversity—the variation in the ecological traits among species within a community or pool of species—is increasingly used to link biodiversity to ecosystem services and environmental change. However, the ecological relevance of functional diversity indices to ecosystem services depends on the selected traits, the species pools, and the questions being asked. In fish ecology, ecomorphological traits such as body shape and mouth position are the most used to describe differences in habitat use and resource acquisition by different organisms. Yet, it remains unclear which traits best represent the ecological variation among species across regional pools of species, and if such traits are indeed related to the ecosystem services fishes provide.
This project aims to evaluate how functional traits and ecosystem services are related across freshwater fish assemblages from distinct biomes. Specifically, the student will help to:
(1) compile trait and ecosystem services data from different regional fish faunas;
(2) learn and apply multivariate analyses to identify key axes of trait variation;
(3) assess the relative contribution and redundancy of traits and ecosystem services across different regional pools of species.
We expect that the traits contributing most to the functional variation among fishes will vary depending on the regional pool of species, which might lead to the low utility of the most commonly used traits in the literature. These findings will help researchers to identify a minimal set of traits that consistently capture functional diversity and the contribution of fishes to ecosystem, contributing to the standardization of trait-based approaches in aquatic conservation and biodiversity assessments. In this project, the student will be integrated into an active research group and gain training in ecological data analysis in R.
Research area, student roles & skills
Research area: One of my research lines focuses on trait-based ecology and its applications to understand how anthropogenic and natural environmental change is affecting the structure of freshwater communities and associated ecosystem services. In this research line, I investigate how ecological traits mediate species responses to environmental change and shape community assembly and ecosystem services across biomes. I use multiple statistical approaches and open ecological datasets to understand patterns of functional diversity, and I am particularly interested in identifying general principles that explain community structure across regional species pools.
Student roles: The student will work closely with the supervisor and lab members to conduct trait-based analyses of regional fish assemblages. Their role will include: 1) conducting literature reviews to identify ecosystem services associated with freshwater fishes; 2) assisting with trait data compilation by reviewing databases and extracting values from published sources; 3) cleaning and organizing species occurrence and trait datasets in R; 4) performing multivariate analyses to ordinate species and identify major axes of trait variation; 5) participating in weekly lab meetings and discussions. The student will be mentored in handling and organizing ecological data, trait-based ecology, and programming applied to biodiversity science. By the end of the internship, the student will have developed transferable skills in data handling and visualization.
Skills required: The student should have a background in ecology, biology, or environmental science and be familiar with ecological concepts related to biodiversity and species ecological niches. Experience with R programming and statistics is strongly preferred. Previous experience with fish biology, community ecology, or multivariate statistics is considered an asset but not required. An interest in biodiversity science is essential.
66. Mapping viromes to microbiomes in thawing permafrost and their implications for the fate of Arctic carbon
Supervisor: Andrew Tanentzap
University: Trent University (Peterborough campus)
Perennially frozen ground, termed permafrost, contains roughly twice the amount of carbon in the northern hemisphere than is currently in the entire atmosphere. Although permafrost has remained frozen for millennia, it is now thawing because of climate warming. Permafrost thaw and the corresponding transformation of stored organic carbon into potent greenhouse gases like CO2 and CH4 are therefore an important feedback onto the global climate. In addition to storing carbon, permafrost soils contain viable microbial assemblages, including cryophiles that can remain active under frozen conditions and dormant cells that may reactivate following thaw. These permafrost-inhabiting microbes play a key role in determining the fate of stored carbon as these soils thaw, making it important to understand the diversity of viable microbes in permafrost and how interactions between these microbes may influence functional processes under thawing conditions. One particularly understudied compartment of permafrost biodiversity is the virome; viruses such as bacteriophages that can influence carbon cycling by limiting growth of their bacterial hosts and causing lysis of their host cells.
This project will identify changes in the microbiomes and viromes of ancient permafrost samples spanning a chronosequence from the Pleistocene into the Holocene, and link viruses to their host microorganisms to characterise host-virus interactions that may impact microbial community assembly and metabolism under thawing conditions. The specific activities will be tailored to the interests of the student, and may include field work to collect permafrost samples from Arctic environments, lab-based methods for metagenomics and single-cell genomics, and bioinformatics approaches for analyzing microbial communities and linking viruses to those of their hosts. There are additional opportunities to incorporate analytical chemistry techniques to link microbial and viral diversity to the composition of organic carbon in permafrost.
Research area, student roles & skills
Research area: Our research group studies the impacts of environmental change on biodiversity and ecosystem services, such as clean drinking water, climate regulation, and food production. These ecosystem services are mediated in large part by microorganisms, including bacteria and fungi as well as viruses, which directly and indirectly influence the transformation and trophic transfer of carbon and nutrients through ecosystems. We merge theory and approaches from biology and chemistry to study how microbial community structure and function change because of climate change and the implications for ecosystem-scale processes. The outcomes of our research will help society mitigate the impacts of climate change
Student roles: The student will work in a team of graduate students and postdoctoral researchers to undertake field and laboratory work related to environmental microbiology, organic matter biogeochemistry, and climate change. Potential field work will involve collecting permafrost samples from Arctic environments in Canada. In the lab, the student will learn techniques for the extraction of nucleic acids from sediments and the preparation of samples for metagenomic sequencing via the PromethION platform from Oxford Nanopore Technologies. The student will also receive training on techniques for cell suspension, flow cytometry, and encapsulation for single cell genomics approaches. Additionally, there will be opportunities to analyse the composition of organic carbon in permafrost samples using one of the most advanced mass spectrometers in the world: a Fourier-transform ion cyclotron resonance mass spectrometer at the Trent University Water Quality Centre. The student will be trained in bioinformatics and statistics to conduct an analysis of permafrost microbiomes and viromes. Depending on the student's interests, they will undertake an independent analysis of how microbial biodiversity in permafrost changes across time, linking taxonomic and functional composition of microbiomes to organic matter composition, or mapping potential pathogen-host interactions that could impact the assembly of microbiomes following thaw. Throughout, the student will learn standard laboratory operating procedures and statistical modelling (including computer programming). The student will also be provided with all relevant health and safety training, including for outdoor field work and first aid in remote locations.
Skills required: The student should be enrolled in a degree program in biology, ecology, chemistry, earth sciences, or a similar discipline. Ideally, the student will already have some laboratory and fieldwork experience and skills in data science (e.g. relevant courses), but training in will be provided by our research group in molecular biology and bioinformatics as well as other relevant analytical techniques.
67. Mechanisms of inhibitory nerve cell wiring in the developing brain
In this project, the student will investigate the development of inhibitory neuron populations in the mouse cerebellum. The objectives of the project are to profile the molecular and morphological signatures of these inhibitory neurons by: 1) injecting viral vectors that label neuronal morphologies; 2) analyzing mice and cerebellum samples that lack molecules of interest, which we hypothesize regulate neuronal wiring; and 3) elucidating the effects on connectivity patterns in the mouse brain. The student will also perform new techniques developed in the lab, including multiplexed RNA imaging and testing new molecular reporters for in vivo synaptic connectivity. The student will receive considerable training in molecular cloning, neuroanatomy, histology, and microscopy techniques. Another opportunity is to perform computational analyses of single-cell profiling and spatial transcriptomic datasets.
Research area, student roles & skills
Research area: Formation of precise connections between nerve cells is critical for proper wiring and functioning of the nervous system, and alterations in these pathways may underlie developing brain disorders. Research in my lab to understand how neurons become organized into neural circuits and to uncover the molecular and cellular pathways that specify their wiring patterns. We are particularly interested in the development of inhibitory nerve in the cerebellum and cortex. We use transgenic mouse models and integrate a variety of approaches such as molecular genetics, confocal microscopy, mouse brain histology, and mouse behavior.
Student roles: The student will be responsible for planning, preparing and executing experiments, with training and supervision. The student will also be responsible for organizing and analyzing data. The student will receive hands-on training for tissue histology (animal models), confocal microscopy and quantitative image analyses.
Skills required: A student with a background in the biological sciences, and who is keen to learn new techniques and interested in microscopy and quantitative image analyses would be well-suited for this project. Background in neuroscience and/or experience in molecular biology lab are assets, but not a requirement.
Genome sequencing and bioinformatic analysis of genomic data from marine prokaryotes and viruses. Depending on the current state of the project, it may include sampling, cultivation and experimental work in addition to the genomic analysis.
Research area, student roles & skills
Research area: We are investigating the interactions between marine protists (single-celled eukaryotes) and the bacteria present in their surrondings. We employ genomics, bioinformatics and molecular biology approaches and our conceptual framework relies strongly on evolutionary biology.
Student roles: The student will join a project aimed to sequence and analyze the genomes of microbes. Activities may include DNA and RNA purifications from tissues and/or cell cultures, performing molecular biology protocols to process the DNA for next-generation DNA sequencing. The student will also assist in processing the sequence data using bioinformatics tools. During the stay, the student will be a regular member of the laboratory and as such, engage in shared lab duties and other activities such as training sessions, lab meetings, seminars etc.
Skills required: Intermediate knowledge of genetics, molecular biology and metabolism (biochemistry). Good computing skills overall, including comfortable use of network tools such as FTP, proper use of spreadsheets to organize data. Familiarity with computer applications for genetics including sequence searches using online tools such as Blast and basic knowledge of sequence file formats. Scripting and UNIX skills are useful. Basic microbiological methods and working in sterility.
The project involves applying the benchmarking protocols from the international resolution benchmarking project, acquiring images on different optical microscopes in the Advanced BioImaging Facility (ABIF) and uploading optical microscopy image data to the International Resolution Benchmarking Platform (RBP). The RBP is a community resource for microscopists to measure and compare the resolution of their microscopes with similar measurements from other instruments around the world. This will allow the ABIF to monitor the quality of the microscopes within the facility, understand if any repairs are needed, understand how stable the microscope performance is and how the microscope performance compares to similar microscopes around the world. The data will also allow us to determine if there are any issues with the optical lenses on the microscopes and if there are, address them in a timely manner. For confocal laser scanning microscopy (CLSM), images will be collected of the fluorescently labelled actin cytoskeleton in fixed cultured cell samples and for super resolution Stimulate Emission Depletion (STED) microscopy of sub-units of the nuclear pore complex revealing 8-fold symmetry if the microscope is well aligned and performing well.
Research area, student roles & skills
Research area: My research focuses on applying advance bioimaging tools to study cell migration in normal and diseased states. We work to understand the molecular mechanisms that regulate cell-matrix adhesions and in turn regulate cell migration. Cell migration is important for normal processes such as the immune response and also diseases such as cancer metastasis and neurological disorders. In order to study cellular systems we need access to robust, reproducible technologies including optical microscopes. Those microscopes need to be carefully quality controlled and benchmarked to ensure the highest quality image data for scientific discovery and innovation.
Student roles: Work independently on the microscopes, acquire images, interact with the RBP, synthesize data and present it to the laboratory group. Will have the opportunity to work with other students and staff in the lab, learn about other ongoing projects studying cell migration, particiapte in group meetings and if possible attend one scientific conference during the internship period.
Skills required: Some experience with optical microscopy would be an asset. Need to read the literature, synthesize information and interpret experimental data. Ability to carefully follow protocols. Ability to follow indepth training programs and work independently on complex optical microscopy platforms. Some experience with quantitative image analysis would be an asset. Ability to take and plot data, build figures and write text to describe trends seen over time, between microscopes and between microscopy modalities (e.g. CLSM vs STED). Ability to work independently and as part of a team.
70. Misbehaving on the edge: Behavioural ecology of an invasive fish, the round goby
The research project will focus on which behavioural traits promote range expansion in invasive species. In particular we will examine how boldness, swimming capacity, affiliation and aggression influence the likelihood of dispersal in round goby, an invasive fish in the Laurentian Great Lakes. Fish will be collected along two different invasion gradients in Canada and individuals from established areas will be compared to individuals from the range edge. We will test fish in dispersal arenas and in swim tunnels and explore how sex, size and reproductive status influences their tendency to move and socialize. There will be a mixture of fieldwork and lab experiments as part of this project. Once the data is collected, we will analyze and visualize the data. The intention is to eventually write up this project for publication.
Research area, student roles & skills
Research area: I am a fish behavioural ecologist which means I study ecological and evolutionary processes using the lens of behaviour. This project is part of a longer research program that examines how invasive species change ecosystem function and what traits lead to their success. The aim of this research program is to identify factors that will help mitigate the harm caused by invasive species.
Student roles: The student(s) will sample and characterize the demography of the invasive round goby populations by comprehensive sampling fish using minnow traps along a 500m edge region and then repeat sampling at established populations where round goby have been present for 20 years or more. This will happen along two invasion gradients in Ontario. All fish caught will be sexed, measured and tissues will be collected for later diet, age, foodweb analyses. A sample of the fish caught will also be used for behavioural and physiological experiments that are part of a larger project. The student intern will help help run fish through swimming tests and dispersal assays and explore how fish sex, fish size and where along the invasion gradient the fish came from influence their behaviour and movement ecology.
Skills required: If you are interested in ecology, animal behaviour, physiology, or conservation biology, this might be the project for you. Ideally we would like to recruit someone who is comfortable around the water and with being outdoors. Having fish handling and capture skills will be an asset but is not a needed skill. If you have an interest and have had any experience with field work or any previous training in ecology, or water quality assessment, or physiological or behavioural analyses that background will also be super helpful but again is not necessary to already have for the position.
71. Molecular and genetic analyses of systemic acquired resistance in plants under global warming conditions
Supervisor: Christian Danve Castroverde
University: Wilfrid Laurier University (Waterloo campus)
Previous studies have shown that high temperatures critically influence plant disease resistance by targeting vital components of the plant immune system. These include diverse mechanisms, including basal immunity, pattern-triggered immunity and effector-triggered immunity. Collectively, these defence responses occur at the local site of pathogen infection. Specifically, we have shown that elevated temperature suppresses plant defences at the local/primary site of infection by decreasing the levels of the immune signal and defence hormone salicylic acid (SA). Although SA is also functionally linked with plant systemic defences (i.e. at distal uninfected sites from the primary infection site), it is currently unclear if and/or how temperature impacts plant systemic immunity. For this purpose, this project aims to investigate how systemic immunity and defence gene expression are regulated at normal and elevated temperatures. We will test and locally infiltrate Arabidopsis leaves with various inducers of systemic immunity, including glycerol-3-phosphate (G3P), azelaic acid (AzA), reactive oxygen species or ROS (hydrogen peroxide) and nicotinamide adenine dinucleotide (NAD+). These are anticipated to induce systemic immunity and defence gene expression. We will then inoculate the model bacterial pathogen Pseudomonas syringae pv. tomato (Pst) DC3000 both locally and systemically. Bacterial numbers and symptom scores will be measured at both normal and elevated temperatures. In addition to physiological analyses, samples will be collected and used for total RNA extraction, cDNA synthesis and gene expression (qPCR) analyses. Because mechanistic details could be missed by merely examining local plant resistance, our discoveries could identify novel temperature-vulnerable genes and phenotypes for future investigations. Overall, this will provide a more comprehensive understanding of the immunoregulatory landscape during systemic and local defence responses in a warming world.
Research area, student roles & skills
Research area: My research program is centred around understanding plant immune signalling and disease resistance in a changing environment. The overarching vision is to: (1) discover novel regulators of temperature-sensitive immunity; (2) determine if temperature-regulated immunity is conserved in diverse species; and (3) develop innovative technologies to repair the immune system and generate climate-smart plants. We integrate multiple approaches, from molecular biology and genetics to microbiology and biochemistry, to investigate the profound impact of changing temperatures on plant immunity and disease. It is anticipated that fundamental discoveries can be leveraged towards disease prevention and climate resilience programs in Canada and globally.
Student roles: The student will be involved in experimentation, data collection and data analyses. They will maintain their own tomato plants and initiate Pst DC3000 bacterial cultures for pathogen inoculation assays. They will inoculate plants with pathogen and collect tissue samples along a time course of infection. The student will use these tomato samples for defence gene expression analyses (RNA extraction, cDNA synthesis and qRT-PCR), SA measurements (biosensor luminescence assay) and bacterial growth determination (colony counting).
Skills required: Required: Background in biology, basic lab experience Preferred (but not required): Advanced skills in molecular biology, microbiology and plant biology
72. Molecular mechanism of cardiac regeneration and cell communication
The adult zebrafish has the remarkable ability to fully regenerate its heart after injury, making it a powerful model for uncovering the fundamental principles of tissue regeneration. This process depends on dynamic interactions between cardiomyocytes and surrounding cell types, which together create a regenerative environment that drives cell dedifferentiation, proliferation, and tissue remodelling. Recent studies have identified cardiomyocyte protrusion and invasion into fibrotic tissue as key cellular behaviours during regeneration. However, directly observing these events in vivo remains challenging due to the limited optical accessibility of adult zebrafish.
This research project offers the opportunity to tackle this challenge by establishing and optimising an ex vivo culture system for adult zebrafish heart slices. This innovative approach will allow long-term live imaging of regenerating tissue, enabling direct visualisation of how cells move, interact, and communicate in real time. By combining this system with advanced transgenic zebrafish lines in which different cell types are fluorescently labelled, the project will explore how cardiac cells coordinate their behaviour, particularly at the injury border zone.
This project is well-suited for highly motivated students interested in developmental biology, regeneration, and cell communication. The student will gain hands-on experience with cutting-edge live imaging, ex vivo tissue culture, and quantitative analysis of cellular dynamics, while contributing to a rapidly evolving field with strong biomedical relevance.
Research area, student roles & skills
Research area: Our lab is interested in the cellular and molecular mechanisms that drive cardiac regeneration, particularly focusing on the cell-cell communication within an injured heart. Our goal is to understand how long-distance signals effectively coordinate cardiomyocyte responses during tissue regeneration. By utilizing zebrafish models, we are combining genetics, molecular biology, histology, and advanced live imaging techniques to gain insights into the interactions between different cell types. This research aims to pave the way for innovative strategies to rebuild functional heart tissue.
Student roles: The intern will primarily be responsible for both experimental work and data analysis. - Performing wet lab experiments using the Zebrafish model - Establishing and optimizing ex vivo heart slice cultures under various conditions - Conducting live imaging experiments - Analyzing and interpreting cellular dynamics and imaging data
Skills required: These are university students; I would suggest adapting: - A strong scientific foundation and solid understanding of biology, genetics, molecular biology, and cell biology. - A solid understanding of regenerative and developmental biology. - Interest in or experience with wet lab experiments (e.g., molecular biology, cell culture, or related techniques). - Candidates should be highly motivated, proactive, and self-driven. - Comfort with failure and iteration, along with persistence in problem-solving. - Ability to read and critically interpret scientific literature. - Previous research experience with zebrafish and/or heart regeneration is advantageous, but not essential.
73. Molecular mechanisms of cardiac regeneration and epicardial crosstalk
Following cryoinjury (myocardial infarction), CMA is rapidly activated during the early phases of cardiac regeneration, with preliminary data suggesting strong CMA activity in epicardial cells. The epicardium plays a central role in heart repair by releasing pro-regenerative signals, supporting vessel remodeling, and coordinating interactions with immune cells that help initiate regeneration.
The aim of the research project is to investigate the role of CMA in the epicardial response after heart injury. The student will: (i) learn the in vitro culturing of epicardial cells from adult zebrafish hearts; (ii) use gain- and loss-of-function fish lines together with pharmacological modulators to modulate CMA in these cells; (iii) use immunohistochemistry techniques to stain for specific cardiac cell markers and assess their dynamics when CMA is altered.
Overall, the project will explore the impact of CMA on epicardial cell migration, proliferation, epithelial-to-mesenchymal transition and immune response during heart regeneration and homeostatic cardiac growth.
Research area, student roles & skills
Research area: Myocardial infarction is a leading cause of death worldwide, resulting in the replacement of damaged myocardium with non-contractile scar tissue and ultimately heart failure. Current treatments delay disease progression but do not restore functional myocardium. Unlike humans, zebrafish can regenerate their heart after injury. Recent studies indicate that specific cell types undergo metabolic rewiring to support this response, yet the mechanisms tuning the levels of metabolic enzymes remain unclear. This project will investigate the role of chaperone-mediated autophagy (CMA), a selective protein degradation pathway, in zebrafish heart regeneration, testing whether CMA modulates metabolic rewiring by targeting key enzymes.
Student roles: After receiving training in the relevant methods, the student will be able to extract zebrafish hearts and culture them on tissue culture plates. Culture medium will be added for the cells to migrate and proliferate. Then, the student will perform immunostainings with specific cellular markers, and acquire images using standard microscopy approaches. Beyond experimental work, the student will be integrated into a collaborative and supportive research environment. They will participate in regular lab meetings, where ongoing projects and methodologies are discussed, providing exposure to a broad range of approaches in cardiovascular biology and regeneration. As the project progresses, the student will be encouraged to develop their scientific communication skills and will have the opportunity to present their work to lab members.
Skills required: We are seeking a motivated student with a strong interest in molecular and/or cellular biology, regenerative medicine, heart repair. The ideal candidate should be curious and eager to learn, with a foundation in experimental biology. An interest for in vitro techniques is highly desirable, as the project will involve cell culture and related laboratory methods.
74. Monitoring Stress and Foraging Condition of Seabirds in Northern Canada
Supervisor: Kyle Elliott
University: McGill University (Montréal campus)
Location: Sainte-Anne-De-Bellevue, Québec
Start date: 2027-07-01 (flexible)
Disciplines: Biological Sciences, Ecology, Science and Technology
Seabirds are the most rapidly declining bird group worldwide. Canada, with the longest coastline in the world, hosts significant numbers of seabirds, including important breeding populations along the Hudson Bay coastline. Arctic and subarctic seabirds in the northern Hudson Bay face mounting pressures from rapid climate warming, shifting sea ice dynamics, changing prey availability, and increasing disturbance from predators and human activity. Understanding how these stressors affect individual birds during the post-breeding period, a critical and energetically demanding phase of the annual cycle, is essential for identifying the mechanisms driving population-level change. This project is focused on the northern Hudson Bay as a study system because it represents one of the most climatically sensitive marine environments in Canada. The project will examine post-breeding movement and physiological stress in arctic seabirds, using tracking and biologging tools alongside stress biomarkers to reveal how birds respond to environmental conditions after the breeding season and before their departure to wintering areas.
Research area, student roles & skills
Research area: Our research group studies the ecology of top avian predators as indicators of the health of the Arctic. We focus primarily on links between physiology, behaviour, and fitness, particularly in the context of a changing climate. Avian predators integrate information over space and time, and our research uses that information to understand climatic, toxicological, and behavioural changes impacting these species in Northern environments. Our research techniques are also used with an eye towards conservation, with indicators of population-level and species-level health as well as broader, large-scale environmental trends.
Student roles: Successful candidates will join a multidisciplinary team contributing to the monitoring of seabird populations in the northern Hudson Bay and additional opportunities for monitoring in the Gulf of St. Lawrence. Research will be lead by Tabatha Cormier (Ph.D. student at McGill University) and Dr. Don-Jean Leandri (Research Scientist at Environment and Climate Change Canada). The project is based out of McGill University. Our team is involved in a number of long-term monitoring and conservation initiatives within the region, including movement tracking and ecotoxicological analysis. These monitoring techniques are applied broadly to many seabird species and in 2027, the team will be focused on the following research objectives: i) establishing baseline isotopic signatures of Thick-billed murres (Uria lomvia) during the non-breeding season from feather samples, ii) determining baseline stress levels, and iii) assessing annual movement patterns of murres.
Skills required: Ideal candidates for this project will have a background in ecology and/or environmental biology. Experience working with or handling animals, particularly birds, is a strong asset, as students must be willing to learn ethical and careful handling techniques and basic seabird identification skills. Some lab work may be required, and candidates should be comfortable following established laboratory protocols. Candidates should be comfortable working in remote field settings with flexible and variable schedules, as tasks and active hours may shift throughout the season. A driver's license is an asset but not required.
For a long time, the central nervous system was considered an immunoprivileged site for its limited arsenal to mount an inflammatory response. In recent years, the work of many researchers throughout the world demonstrated that the immune system plays a critical role in maintaining CNS homeostasis during a healthy state and disease. Neuroinflammation emerged as a common denominator in many neurodegenerative diseases, including multiple sclerosis, Parkinson’s disease, Alzheimer’s disease, amyotrophic lateral sclerosis, Huntington’s disease, epilepsy, stroke, etc.
In our lab, we study neuroinflammation on different levels, including cellular molecular biology, classic immunology and neurosciences, and computerized animal behavior assessment using a state-of-the-art behavior recognition system that will facilitate break-through discoveries in the domain of neurodegenerative diseases.
Recently we initiated human studies that will investigate the potential of wearable devices in the diagnosis and prognosis of MS.
The results of these studies will lay the foundation for the development of new therapeutic agents to treat neurodegenerative diseases.
Research area, student roles & skills
Research area: Immunology, Neuroscience, Bioinformatics
Student roles: The projects range from simple bench work to advance bioinformatics. See what suits you the most. Most importantly be friendly and help others. .
Skills required: We are looking for students with general science background and knowledge of molecular biology. In addition, we are developing a project detecting animal movements, so if you know Python we can have fun in programming. Most importantly, we are looking for highly motivated, enthusiastic people with excellent academic standings and good team players.
76. Neuropeptides and other neuronal factors regulating critical physiological processes in insects including disease vectors and agricultural pests.
The current project will include functional examination of neuropeptides and other endocrine regulators involving molecular and physiological investigations of the origin, regulation and mechanism of action on cognate receptors that are often GPCRs. Specifically, this research project will explore the physiological roles of signaling molecules derived from the nervous system and peripheral endocrine glands, with a particular focus on neuropeptides, which are often released into circulation as hormones and regulate numerous processes by controlling the activity of visceral organs including the intestine and reproductive organs. This research aims to discover and characterize neurohormone systems controlling processes related to feeding, digestion and absorption, metabolism, excretion, hydromineral balance, reproduction and growth in insects disease vectors (mosquitoes), agricultural pests as well as model organisms (life the fruit fly). An integrative approach is utilized investigating neuroendocrine systems at multiple levels of complexity, which allows the intricate details to be more readily realized and then integrated into the context of the whole organism. We combine a plethora of techniques to localize the receptors of these neurohormones since target organ identification helps to realize the roles of these hormones.
Given the 12 week length of this research internship, the successful applicant and principal investigator will discuss in more detail what the focus of the research will be, which will also depend on the intern’s start date and specific research interests (the point is that there will be some flexibility). Recent evidence of successful implementation of a similar research project includes our studies on CAPA-related peptides, which we found activate their cognate receptor forming an essential anti-diuretic regulatory system in the mosquito, Aedes aegypti (Sajadi et al., 2018 J. Exp. Biol; Sajadi et al., 2020 Sci. Reports; Sajadi et al., 2023 PNAS). As a comparative physiologist, I also welcome studying other insects!
Research area, student roles & skills
Research area: Neuropeptides and their receptors play a central role in the regulation of most physiological processes in animals. Our laboratory studies the function of neuropeptides and their receptors in insects. To understand the role and importance of distinct neuropeptide systems, we firstly deorphanize and functionally validate the activity of neuropeptidergic ligands on their prospective receptors, which we accomplish using heterologous high-throughput techniques. Once ligand/receptor pairs are validated, we next examine their physiological roles in the organism using various tools that may involve combining reverse genetic approaches with in vivo and/or in vitro bioassays.
Student roles: The role of the successful applicant will involve either molecular or physiological techniques to validate neuropeptide receptor expression profiles, which will unravel the target organs of the specific neuropeptide. With target organs identified, the trainee will use techniques that are routinely used in the lab to examine the activity of hormones and other neuronal factors on transport of primary ions (e.g. Na+, K+) across regions of the gut, controlling myoactivity, or in regulation of reproductive biology. Relatedly, immunoreactivity of specific neuropeptides and other protein targets may be utilized to examine the association of relevant co-factors in signalling and function. These research experiences will provide the student with solid hands-on training that will be important for a variety of career paths. Based on the dedication and perseverance of the intern along with the amount and quality of the research data collected during the internship, the student could be invited to continue research through another research opportunity including future graduate positions in the lab if interested. The student intern involved in this research may also gain authorship in any resulting manuscript(s) related to their work. More information on previous research publications can be found at: https://paluzzi.lab.yorku.ca
Skills required: The student should have completed and achieved a grade of at least 75% in a second or third year undergraduate (i) animal (or human) physiology and (ii) molecular biology courses. Prior experience with working in a relevant research lab would be beneficial, but is not a requirement. The successful internship student will be provided with all the necessary training to learn how to carry out the particular experimental techniques required for the finalized research project.
77. Plant Community Recovery After disturbance in boreal forests
Supervisor: Anne McIntosh
University: University of Alberta (Edmonton campus)
Over the past two decades, Alberta’s lodgepole pine (Pinus contorta var. latifolia Engelm.) forests have experienced significant perturbations due to the combined impacts of extensive mountain pine beetle (Dendroctonus ponderosae Hopkins) outbreaks and increasingly frequent wildfires. The eastward expansion of the mountain pine beetle across the Rocky Mountains in 2005 initiated widespread infestations within Jasper National Park between 2016-2018. Concurrently, wildfires have inflicted substantial ecological and economic damage across Alberta, as the recent experienced Jasper Wildfire 2024, an historic wildfire that affected over 32,000 hectares, including burning a portion of historic Jasper city. A comprehensive understanding of the long-term recovery of forest properties and the trajectory of overall forest succession following mountain pine beetle and wildfire disturbances in western Alberta lodgepole pine forests remains limited. The intern(s) on this project will work with my graduate students (PhD student Larissa Villasenor, MSc to be confirmed) and potentially other research assistants in the McIntosh Ecological Recovery lab to evaluate recovery trajectories of understory vegetation and forest succession following diverse ecological stressors. Larissa, a former Globalink Mitacs intern from Mexico, will be in her third year of her PhD and will have completed two field sampling seasons and the MSc student will be doing his first field season. The specific project details and sampling sites will be determined in winter 2027 based on MSc thesis plans not yet finalized. Studies will focus on collecting understory plant community data, including functional trait properties, at boreal forests across a range of disturbance intensities (e.g., wildfire, mountain pine beetle) in western and northern Alberta, and are expected to include sites within Jasper National Park. A lot of time will be spent doing field data collection, and remaining time doing data entry, office-based research, and lab work when not in field. Details to be confirmed at interview.
Research area, student roles & skills
Research area: Disturbance is an important ecological driver that alters the environment and spatio-temporal distribution of ecosystem resources; these in turn influence the composition and diversity of plant communities. The combined influences of increasing anthropogenic disturbance and novel disturbances associated with global change are pushing the structure and function of northern forested ecosystems beyond their historical natural ranges of variability. My research program focuses on understanding the mechanisms underlying changes in patterns and successional trajectories of northern plant (and sometimes soil microbial) communities by examining responses to disturbances that capture a gradient of severities (both anthropogenic and natural).
Student roles: You will play an active role, primarily in the field, but also in office and likely lab settings. You will spend a large portion of time as part of a 2-4 person crew with PhD and or MSc student supervisor collecting data at forest research sites. The primary research sites will be driving distance from a city/town where you will be based during the time in the field sampling (e.g., Jasper). Expenses covered for intern when remote in the field (housing, food). In the field, you will gain experience in navigating to sites, study design & plot lay-out, vegetation sampling techniques & will learn some plant ID skills. In the lab, you will gain experience in processing of samples, including organizing plant samples and completing plant trait analyses and/or preparing samples. You will also be involved in preparation for field sampling, including organizing supplies and sampling equipment and preparing field datasheets. Intensive field training for field activities will be provided. Time may also be spent by you gathering and reviewing relevant literature related to the research program, entering data collected in the field into spreadsheets (e.g., MS Excel), and potentially doing some preliminary analysis. In addition you will be exposed to other field and literature research that is ongoing in the lab. Overall you will be exposed to diverse experiences in the McIntosh lab, but with an emphasis primarily on field research activities. Note Anne is in Camrose at Augustana Campus (1 hr S from Edmonton) but YOU will be based in Edmonton b/c it is where the graduate students are based and closer to field sites. We will still meet regularly as a lab and Anne will come out to the field at some points during field sampling campaigns. We will know more specifics when MSc project is finalized.
Skills required: 1. Background in botany/ecology/related field. 2. Willingness to work long hours & atypical schedule throughout summer when field work is conducted, including when weather may be sub-optimal (e.g., raining but safe) and you may encounter wildlife (e.g., bears - although very unlikely!) and be ok with annoying insects (e.g., mosquitoes). 3. Previous field work experience (including data collection, GPS navigation, working remote conditions) would be an asset but not required. 4. Training on field sampling (e.g., field research safety orientation) and plant analysis will be provided for you. 5. Attention to detail. 6. Positive attitude and be a team player.
78. Prediction of Cancer Immunotherapy Response Using Machine Learning and Tumor Microenvironment Signatures.
Supervisor: Nisha Puthiyedth
University: Thompson Rivers University (Kamloops campus)
This research project focuses on using machine learning and bioinformatics approaches to predict how cancer patients may respond to immunotherapy treatments. The goal is to develop computational methods that can help identify patients who are more likely to benefit from immune checkpoint inhibitor therapies based on genomic and tumor microenvironment data.
The student will work with publicly available cancer genomics and transcriptomics datasets that include biomarker and treatment response information. The project will involve data cleaning, exploratory analysis, statistical evaluation, and development of machine learning models for predictive analysis.
A major part of the project involves studying biologically important biomarkers linked to immunotherapy response, such as immune cell activity, immune checkpoint expression, and tumor microenvironment patterns. The student will help compare different machine learning approaches, evaluate model performance, and create visualizations to better understand predictive patterns in the data.
The project also focuses on building reproducible and interpretable computational workflows to improve reliability and transparency in AI-assisted cancer research. Depending on the student’s interests and background, the internship may also include external dataset validation and comparative modelling experiments.
This research combines artificial intelligence, computational biology, and precision medicine to address important challenges in cancer immunotherapy research. Expected outcomes include reproducible analysis pipelines, predictive modelling results, biological data visualizations, and a final research report. Findings from the project may contribute to future conference presentations or peer-reviewed publications in AI-assisted precision oncology research.
Research area, student roles & skills
Research area: My research focuses on artificial intelligence, bioinformatics, and computational approaches for precision oncology and biomedical data analysis. I work with genomic, transcriptomic, and tumor microenvironment datasets to develop machine learning models for predicting cancer treatment response and identifying clinically relevant biomarkers. My research integrates statistical modelling, interpretable AI methods, and reproducible computational workflows to support data-driven cancer research and precision medicine applications. I am particularly interested in combining bioinformatics and machine learning approaches to improve the prediction of immunotherapy response and translational cancer research.
Student roles: The student intern will participate in the development and evaluation of machine learning models for predicting cancer immunotherapy response using genomic and tumor microenvironment biomarker datasets. The student will contribute to multiple stages of the computational research workflow, including data preprocessing, feature engineering, exploratory analysis, predictive modelling, and model evaluation. The student will work with publicly available transcriptomic and cancer genomics datasets to analyze biomarker signatures associated with immunotherapy response. Responsibilities will include implementing baseline machine learning models, conducting comparative computational experiments, evaluating model performance, and generating visualizations to support interpretation of biological and clinical patterns. The student will also assist in developing reproducible computational workflows for cancer biomarker analysis and precision oncology research. Depending on the student’s interests and background, the project may include external validation experiments, interpretable AI methods, or AI-assisted biomarker analysis approaches. Throughout the internship, the student will gain hands-on experience in machine learning, bioinformatics, computational oncology, and biomedical data analysis. The student will additionally contribute to documentation, literature reviews, technical reports, and dissemination of findings through presentations or future publication activities. This internship will provide interdisciplinary research experience at the intersection of artificial intelligence, cancer genomics, and precision medicine while contributing to the development of AI-assisted tools for precision oncology research.
Skills required: The ideal student should have a background in computer science, bioinformatics, data science, computational biology, or a related field. Experience with programming languages such as Python or R, statistical analysis, and machine learning concepts is preferred. Familiarity with biological data analysis, genomics, transcriptomics, or cancer-related datasets would be considered an asset. Students with experience or interest in artificial intelligence, predictive modelling, or biomedical data science are encouraged to apply. Knowledge of Linux environments, GitHub, data visualization tools, or machine learning frameworks such as Scikit-learn, TensorFlow, or PyTorch would also be beneficial.
79. Process optimization and validation of antibody conjugation reactions
This project involves development and optimization of antibody conjugation reactions and developing analytical protocols to validate the reactions. The antibody biomolecule conjugate contains three primary components, an antibody, a linker molecule and a biomolecule. The linker facilitates the site-specific conjugation of the biomolecule to the antibody. These antibody conjugates have significant applications in both therapeutic and diagnostic fields, including targeted drug delivery, immunoassays, and molecular imaging.
Research area, student roles & skills
Research area: Engineering and bioproduction of recombinat proteins, single cell microfluidic technologies (RNA), Biomaterials and organoid culture.
Student roles: The students participating in this project will: 1. Perform and optimize the conjugation reaction conditions such as molar ratios, temperature and reaction time. 2. Evaluate appropriate linker chemistries for efficient and stable conjugation. 3. Develop and validate analytical protocols, particularly HPLC-based methods. 4. Conduct computational studies such as antibody conjugation docking. This hands-on experience will provide students with valuable skills in bioconjugate chemistry, analytical method development and biochemical analysis.
Skills required: Educational background: Biotechnology, Chemical engineering, Biomedical Engineering, Molecular Biology and other related domains. Laboratory skills: Familiarity with basic laboratory techniques such as pipetting, buffer preparation, pH adjustment etc. Analytical techniques (preferred but not mandatory): High Performance Liquid Chromatography (HPLC) and SDS-PAGE is advantageous. Data analysis and documentation.
Small-diameter vascular grafts are used when patients require blood vessel replacement or bypass surgery, but many synthetic grafts fail because they do not develop a healthy endothelial lining. One barrier to healing is the formation of dense fibrin layers on the graft surface after blood contact. Fibrin is essential for clotting, but when its structure becomes overly compact, it may block cell migration, alter inflammation, and prevent proper integration of the graft.
This project will investigate how fibrin architecture can be controlled on biomaterial surfaces to support vascular graft healing. The intern will help test how experimental conditions and peptide-based modulators affect fibrin formation, structure, and stability. Depending on training and progress, the project may include fibrin turbidity assays, clot formation on biomaterial surfaces, fluorescence or brightfield microscopy, image analysis of fibrin structure, literature review, and basic statistical analysis.
The project is designed as a 12-week undergraduate research experience with clear milestones: initial training and literature review, experimental assay setup, data collection, image/data analysis, and preparation of a final report or presentation. The intern will gain experience in translational cardiovascular biomaterials research and will contribute to a broader program aimed at developing improved strategies for vascular graft endothelialization and regenerative medicine.
Research area, student roles & skills
Research area: My research focuses on regenerative biomaterials and peptide-based strategies to improve cardiovascular repair. A central goal is to understand how fibrin, the main structural protein of blood clots, forms on biomaterial surfaces and how its architecture influences endothelialization, inflammation, and vascular graft healing. We combine protein biochemistry, biomaterials engineering, microscopy, image analysis, and pulsatile-flow models to study clot structure under physiologically relevant conditions. This work aims to develop new approaches to improve the healing and long-term performance of small-diameter vascular grafts.
Student roles: The intern will contribute to a focused experimental project studying how fibrin structure forms on biomaterial surfaces and how this structure can be modulated to improve vascular graft healing. Under supervision, the student will first complete laboratory safety training and review key literature on fibrin, blood-contacting biomaterials, and vascular graft endothelialization.
The student will then assist with preparing experimental materials, running fibrin formation assays, collecting turbidity or microscopy data, and documenting experimental conditions in a laboratory notebook. Depending on experience and project progress, the intern may also help analyze fibrin images using ImageJ/Fiji or related software, quantify structural features such as clot density or fiber organization, and organize results for comparison across experimental conditions.
The intern will meet regularly with the supervisor and research team to discuss progress, troubleshoot experiments, interpret data, and plan next steps. By the end of the internship, the student will prepare a short written report and/or oral presentation summarizing the research question, methods, results, limitations, and future directions. The role is intended to provide hands-on training in translational biomaterials research while generating useful preliminary data for ongoing studies in cardiovascular regenerative medicine.
Skills required: The ideal student will have a background in biochemistry, biomedical science, biomaterials, bioengineering, chemical engineering, biotechnology, or a related field. Experience with basic wet-lab techniques, protein assays, microscopy, image analysis, or cell/biomaterial experiments would be an asset but is not mandatory. The student should be organized, careful with experimental work, interested in cardiovascular or regenerative medicine research, and comfortable reading scientific literature. Basic data analysis skills using Excel, GraphPad Prism, ImageJ/Fiji, or similar tools would be helpful.
81. Proteomics analyses in rhabdomyosarcoma
Supervisor: Marie-Claude Sincennes
University: Université INRS (Laval campus)
Location: Chicoutimi, Québec
Start date: 2027-05-03 (flexible)
Disciplines: Biological Sciences, Biochemistry, Biology, Genetics, Medical Sciences, Molecular Biology
In this research project, the intern will identify protein-protein interactions that are crucial for rhabdomyosarcoma cell survival, proliferation and differentiation. The student will perform data mining, proximity-labelling, and co-immunoprecipitation experiments. The intern will get familiar with cell culture, protein and RNA extraction, Western blotting, RT-qPCR and imunofluorescence.
Research area, student roles & skills
Research area: We are using molecular and cellular biology techniques to better understand the biology of muscle stem cells and to identify mis-regulations taking place in muscle-related diseases such as muscular dystrophy and rhabdomyosarcoma.
Student roles: The student will directly be involved in the project. He/she will perform the experiments, the analysis and results interpretation. He/she will be paired with a senior student in the lab. At the end of the internship, the student will present the project results to the team.
Skills required: The most important skill is your motivation! If you know the principle of the different techniques listed above, it will be easier for you. We are looking for interns who are curious, interested, with a will to learn and to do teamwork.
82. Pumpkinseed sunfish parental analysis
Supervisor: Malgorzata Gazda
University: Université de Montréal
Location: Montreal, Québec
Start date: 2027-05-03 (flexible)
Disciplines: Biological Sciences, Biology, Molecular Biology, Genetics, Computer Science, Veterinary Science and Medicine
To understand how reproductive cues are linked to behaviour in pumpkinseed sunfish, we will generate genomic sequencing data from previously collected individuals belonging to known family groups. By sequencing these fish, we will be able to characterize the genetic variation present within and among families and determine how inherited genetic differences contribute to behavioural diversity.
This approach will allow us to test whether specific families share genetic signatures associated with particular reproductive traits — such as nest‑building intensity, parental care strategies, aggression toward intruders, or responsiveness to environmental cues that trigger reproduction. By integrating genomic data with behavioural observations and ecological measurements, we can identify whether certain behavioural phenotypes are strongly heritable, whether they arise from gene–environment interactions, or whether they reflect plastic responses modulated by underlying genetic predispositions.
Ultimately, this project will reveal how genetic architecture shapes reproductive behaviour in a species where males exhibit complex and variable reproductive strategies. Understanding these links between genotype and behaviour will provide new insights into the evolution of mating systems, the maintenance of behavioural diversity within populations, and the ecological factors that influence reproductive success in freshwater fish.
Research area, student roles & skills
Research area: In my research group we combine population genomics, classical genetics, biochemistry, physiology, molecular biology, phylogenetics, neurobiology and behavior. The interdisciplinarity is achieved thanks to wide range of collaborations as well as our willingness and courage to explore unknown. We work on wide variety of organisms from birds to plants, with main model species of canary birds. Other than genotype phenotype association we are also interested in understanding how gene function and expression evolve across species as well as how is it impacted by organismal biology, physiology and environment.
Student roles: Candidates depending on their experience and interest will be able to develop the bioinformatics skills, by managing the sequencing data, analysis of the sequencing data and performing parental analysis. Additionally there will be opportunity to learn practical skills in molecular biology lab, such as extracting DNA, preparing sequencing libraries, qPCR. For interested candidates potential to mix both, so the bioinformatics combined with the wet lab. Each intern will work on their own small project, that later will be implemented in the larger work. Each student will be expected to write a report based on their projects as well as present it at the group meeting.
Skills required: Candidates for the internship should be interested in evolutionary biology. Basics of scripting/basic programming will be beneficial but not required. Previous wet lab experience for a candidate in more laboratory experience will be beneficial. General understanding of evolutionary biology, genetics and genomics or computer science will be helpful in order to work in an interdisciplinary team with background in biology and computer science. Students should be willing to work collaboratively as a part of the team, with professionalism, attention to details and eager to learn.
To investigate how parasitism influences population structure in pumpkinseed sunfish, we sampled individuals from five lakes that differ markedly in their levels of parasitic infection. These lakes are potentially interconnected through natural waterways, which raises an important ecological question: do fish disperse among lakes, or are populations genetically isolated despite geographic proximity?
Using genomic data generated from these samples, we will characterize patterns of population structure, gene flow, and connectivity among lakes. This will allow us to determine whether movement between lakes occurs regularly, whether dispersal is restricted, or whether parasite pressure contributes to population differentiation.
In parallel, we will integrate these genomic patterns with detailed phenotypic data on parasite load and infection severity collected from each individual. By linking genetic structure with infection profiles, we can test whether populations exposed to higher parasitic pressure show signatures of local adaptation, such as: allele frequency shifts associated with immune function or genomic regions under selection related to parasite resistance or tolerance.
This combined ecological–genomic approach will help identify candidate genes involved in host–parasite interactions and reveal whether parasite-mediated selection is shaping the evolutionary trajectories of these populations.
Ultimately, this project will improve our understanding of how parasitism influences population connectivity, adaptive divergence, and the maintenance of genetic diversity in freshwater fish. These insights are essential for predicting how natural populations respond to ecological stressors and for informing conservation strategies in freshwater ecosystems increasingly affected by environmental change.
Research area, student roles & skills
Research area: In my research group we combine population genomics, classical genetics, biochemistry, physiology, molecular biology, phylogenetics, neurobiology and behavior. The interdisciplinarity is achieved thanks to wide range of collaborations as well as our willingness and courage to explore unknown. We work on wide variety of organisms from birds to plants, with main model species of canary birds. Other than genotype phenotype association we are also interested in understanding how gene function and expression evolve across species as well as how is it impacted by organismal biology, physiology and environment.
Student roles: Candidates depending on their experience and interest will be able to develop the bioinformatics skills, by managing the sequencing data, analysis of the sequencing data. Additionally there will be opportunity to learn practical skills in molecular biology lab, such as extracting DNA, preparing sequencing libraries, qPCR. For interested candidates potential to mix both, so the bioinformatics combined with the wet lab. Each intern will work on their own small project, that later will be implemented in the larger work. Each student will be expected to write a report based on their projects as well as present it at the group meeting.
Skills required: Candidates for the internship should be interested in evolutionary biology. Basics of scripting/basic programming will be beneficial but not required. Previous wet lab experience for a candidate in more laboratory experience will be beneficial. General understanding of evolutionary biology, genetics and genomics or computer science will be helpful in order to work in an interdisciplinary team with background in biology and computer science. Students should be willing to work collaboratively as a part of the team, with professionalism, attention to details and eager to learn.
84. Quels sont les impacts des bryophytes sur le recrutement des plantes vasculaires natives et envahissantes post-perturbation ?
Les mousses des sols forestiers sont bien placées pour jouer des rôles clés dans les rétroactions entre les plantes et les sols et leur importance dans le fonctionnement des écosystèmes, incluant le recrutement des plantes vasculaires, est de plus en plus reconnue dans les forêts boréales nordiques. On ne sait pas encore si ces contributions demeurent importantes à la limite sud de la forêt boréale, où la composition des forêts devient mixte avec des espèces à feuilles caduques. Le Nouveau-Brunswick (Canada) se situe dans cette zone de transition et l’industrie forestière y est très active. Les perturbations entrainées par l'exploitation forestière qui exposent le sol minéral, en altérant entre autres le couvert de mousses, peuvent créer des opportunités pour l'établissement d'espèces exotiques. Les bryophytes peuvent avoir des effets facilitateurs, inhibiteurs ou neutres sur la germination des plantes. Sur un site récemment exploité, des carottes (15cm de diamètre) de sol minéral exposé, de mousses pionnières, de mousses acrocarpes et de mousses hypnacées seront récoltées (5 réplicats/espèce/substrat). Le pourcentage de couverture des différents substrats et la présence d’espèces exotiques seront notés. Puis, dans le cadre d'expériences en chambre de croissance, nous comparerons la germination de quatre espèces exotiques trouvées après la récolte forestière au Nouveau-Brunswick (Plantago major, Ranunculus acris, Taraxacum officinale, Veronica officinalis) et d'un arbuste très envahissant dans la région (Frangula alnus) sur les différents substrats. On s’attend à ce que les espèces à grosses graines tolèrent une plus grande variété de conditions que les espèces avec des graines plus petites. Les mousses devraient inhiber la germination par rapport au sol minéral. Ce projet permettra de mieux évaluer comment les perturbations des sols forestiers et des communautés de mousses lors de la récolte peuvent altérer les probabilités de recrutement naturel de plantes natives et exotiques.
Research area, student roles & skills
Research area: Je suis une écologiste des communautés végétales, avec des champs d’intérêts portant sur la biogéochimie, les perturbations et la succession, ainsi que sur les rétroactions entre les plantes et le sol et entre les plantes et leur microbiote, la fixation de l’azote et les traits fonctionnels. Les recherches dans mon laboratoire portent sur l'écologie des bryophytes et des plantes de sous-bois dans la forêt boréale, de sa limite sud avec la forêt tempérée jusqu'à sa limite nord. Nous utilisons aussi le bryomonitoring afin de cartographier le dépôt atmosphérique de polluants dans la province à l’aide des mousses.
Student roles: Sous la supervision de la professeure Jean, l’étudiant sera responsable de mener à bien le projet de recherche. Après avoir complété toutes les formations nécessaires pour effectuer le travail de manière sécuritaire, l’étudiant participera à l’identification de sites de récolte forestière appropriés, à l’évaluation sur le terrain de l’abondance des différents substrats et la présence d’espèces exotiques, et à la cueillette des différents substrats (sol et mousses) pour les placer en pot. Puis, il sera responsable d’installer l’expérience de germination en laboratoire, incluant des tests de germination dans des plats de pétri pour évaluer la viabilité des graines des différentes espèces, et des tests en pot sur les différents substrats. L’étudiant aura la charge de maintenir cette expérience pendant quelques semaines en allant arroser et déplacer les pots et les pétris à chaque jour, tout en récoltant les données sur les taux de germination. La gestion des données sera effectuée par l’étudiant. À travers ces tâches, l’étudiant effectuera une courte revue de littérature afin de rédiger un rapport présentant ses résultats à la fin du stage. De plus, l’étudiant apprendra à utiliser des statistiques pour interpréter les résultats obtenus. Il est possible que l’étudiant soit amené à aider l’échantillonnage sur le terrain par des étudiants gradués dans le groupe de recherche, ce qui offrira des opportunités d’apprentissage et de développement professionnel supplémentaires. L’étudiant devra aussi participer activement aux rencontres hebdomadaires de laboratoire (club de lecture, mises à jour sur différents projets, ateliers sur différents sujets, etc.).
Skills required: Bien que la majeure partie des connaissances soit acquise à travers le stage, une connaissance de base et un intérêt pour la biologie, écologie et botanique est préférable (études en biologie, botanique ou environnement). L’étudiant doit être prêt à effectuer des travaux en milieu extérieur sous des conditions météorologiques variables. Ce projet nécessite aussi une personne responsable, autonome, organisée et méticuleuse, puisque la personne aura la charge de maintenir les expériences de germination en laboratoire et de faire la collecte et la gestion des données. Une compréhension de base de design expérimental et d’analyses statistiques est souhaitable, mais pas nécessaire.
85. Reconstitution du régime des feux et de la végétation passés à partir d'une étude paléoécologique
The aim of this research project is to reconstruct the past dynamics of forest fires and vegetation over the last few millennia, using sediment cores taken from a lacustrine ecosystem within the mixed boreal forest. The study aims to establish a baseline for the natural variability of disturbances and vegetation in the study area — the Lac Duparquet Teaching and Research Forest — in order to better anticipate how fire regimes will respond to future climate change.
Research area, student roles & skills
Research area: - Reconstruction of Holocene fire regimes using wood charcoal - Reconstructing vegetation dynamics through pollen grain analysis - Analysis of lake sediments and identification of charcoal and fossil pollen grains to reconstruct the history of forest fires during the Holocene. - Study of natural disturbances affecting boreal forest ecosystems using palaeoecological approaches.
Student roles: The student will assist postgraduate students and research professionals with data analysis and management. Depending on their level of independence and initiative, they may be entrusted with responsibility for part of the field sampling. The student will also be involved in data entry and laboratory analyses. Given that there are several ongoing projects in the same region, the student may occasionally assist other teams in the field, which will help to broaden their training. They will also have access to the FERLD station’s facilities, including the dendrochronology and palaeoecology laboratories. Before the fieldwork begins, students must complete a free training course on forest safety and survival, provided by the university. All daily trips will be supervised and monitored by the university’s security staff and the project supervisor.
Skills required: - Education or experience in forest ecology, palaeoecology or plant biology. - Basic computer literacy. Skills in mapping (GIS) would be an advantage. A keen interest in working in forestry and laboratory environments is essential, as are good team spirit and the ability to work independently. - Holding a valid driving licence and being at least 21 years old are additional advantages.
86. Reconstructing long-term environmental changes in boreal forest lakes
Supervisor: Carsten Meyer-Jacob
University: Université du Québec en Abitibi–Temiscamingue (Rouyn-Noranda campus)
Canada's boreal forest contains one of the highest densities of lakes, wetlands, streams, and rivers globally. This ecologically significant landscape is increasingly threatened by resource extraction activities such as forestry and mining. Lakes are closely interconnected with their surrounding terrestrial environments, and both natural and anthropogenic forest disturbances — wildfires, insect outbreaks, logging, and mining — can significantly alter the export of nutrients and organic matter from forest soils to aquatic systems. These disruptions in elemental cycling can have far-reaching consequences for lake ecosystems, influencing water quality, aquatic productivity, and carbon fluxes to the atmosphere.
While monitoring studies have documented the short-term impacts of such disturbances on aquatic environments, a major limitation remains: the lack of long-term observational data beyond a few years. This gap restricts our ability to understand the sustained effects of forest disturbances on boreal lakes and to predict future environmental responses.
This project investigates forest–lake interactions and the long-term effects of various forest disturbances on boreal lakes over the past 200 years, using lake sediments as natural archives of environmental change. These sediments preserve a wealth of physical, chemical, and biological information, enabling reconstruction of both lake and landscape histories over decadal to centennial timescales.
Project objectives:
i) To assess how forest disturbances have influenced lake-water quality and productivity over long time periods; and
ii) To evaluate the implications of these changes for the role of boreal lakes in the terrestrial–aquatic carbon cycle.
Research area, student roles & skills
Research area: I am an environmental biogeochemist specializing in paleolimnology — the study of past environmental changes recorded in lake sediments. My research investigates how climate change, land-use transformation, and pollution shape the cycling and transport of elements between northern lakes and their surrounding landscapes. A central theme is understanding how boreal forest disturbances — including wildfires, insect outbreaks, and human activities — alter vegetation cover and hydrological pathways, and consequently influence organic matter and carbon cycling in these systems. This work sheds light on how northern ecosystems have responded to past changes and what this means for their future.
Student roles: During the internship, the student will support graduate students in both field and laboratory work while undertaking a small independent research project aligned with ongoing studies in the research group.
Responsibilities and Learning Opportunities: - Participation in fieldwork activities, including sediment core collection from boreal lakes - Hands-on training in geochemical, spectroscopic, and paleoecological methods - Preparation and analysis of samples for the independent project - Conducting literature reviews relevant to the project - Writing a brief report summarizing project findings - Attending and contributing to regular team meetings - Developing communication skills through project presentations - Completing required safety and training courses prior to field and lab work
Additional Information: The student will have the opportunity to stay at the research station at the Lake Duparquet Research and Teaching Forest — a unique setting that offers direct immersion in the boreal forest environment central to this research.
Skills required: Applicants should be enrolled in an undergraduate program in Earth Sciences, Environmental Sciences, Biology, or a related field. A strong interest in paleoenvironmental reconstruction, aquatic ecosystems, and biogeochemistry is essential. Some lab and fieldwork experience is an asset, though a demonstrated enthusiasm for both is equally valued. The ideal candidate is motivated, curious, and passionate about understanding environmental change. Strong ability to work both independently and collaboratively is required, as is proficiency in written and spoken English.
The aim is to collect and analyze remote sensing data (i.e. satellite or phenocam) to monitor and/or describe the growth dynamics of tree species in Quebec, Canada.
Research area, student roles & skills
Research area: Smart forests and new technologies in the field. Global changes. Timings and dynamics of tree growth. Tree phenology. Adaptations to local climates, assisted migration of species. Maple syrup production.
Student roles: Student will be asked to (1) extract data from remote sensing images; (2) define leaf phenology or forest health, and (3) combine the variables using statistical modelling. Given the innovative nature of the work, all tasks will be performed by working with the supervisor and some expert lab members.
Skills required: Expertise in geomatics and remote sensing, and related software. The task is challenging because it needs imagination and efficiency. The candidate will have the chance to attend field activities to visit our sample sites on the fascinating forest ecosystem of Canada
88. Resolving Plant Growth and Resiliency Targets
Supervisor: Richard Uhrig
University: University of Alberta (Edmonton campus)
Daily plant processes are underpinned by a series of transcription factors that precisely time the expression profiles of 1000’s of genes throughout the day. One of the main aims of our lab is to understand how these changes translate to protein-level responses to impact daily plant cell processes using a number of classic molecular-biochemical approaches in addition to high-throughput systems-level techniques. Our pursuit of a protein-level understanding of daily plant cell processes has resulted in the identification of a number of targets that are being investigated for improved plant growth and resiliency. Through our use of gene deficient plant lines, we validate hypotheses to better understand how each target integrates into broader plant biology. The targeted validation work that you will pursue will then be carefully analyzed to better resolve potential time-of-day response pathways that can lead to increased growth or resiliency outcomes.
Research area, student roles & skills
Research area: The focus of the Uhrig lab is understanding how plants undertake different cellular processes throughout the day and respond to their environment from a time-of-day perspective. The lab is a collection of molecular-biochemical, functional genomic-systems and computational biologists studying the plant cell signaling mechanisms that control time-of-day cell processes with the ultimate goal of producing climate resilient crops.
Student roles: This project represents an independent role within the Uhrig lab research environment, offering a tangible opportunity for research ownership and broader contribution to the publication of lab research. With the goal of the project being to characterize identified candidate growth and resiliency proteins, the student’s results will feed into the broader research program already underway in order to determine mechanism(s) of action. Correspondingly, any results generated by the student will be appropriately credited to them. The successful applicant can expect to work as an independent researcher within a supportive and hardworking team. As is the case with all Uhrig lab trainees, the student will be is expected to give a series of lab meeting presentations and a short presentation at the end of the project to describe progress made and key results found. The student will also be expected to actively participate in all lab meetings.
Skills required: The ideal candidate will have a background in any of the following areas: plant biology, plant stress biology, cell and molecular biology / biochemistry. Experience with plant handling and common molecular biology techniques would be an asset, though lab specific techniques will be taught. The project will also involve cloning of expression vectors for either plant or recombinant bacterial expression, affinity purification mass spectrometry, and western blotting, so experience with any of these techniques would also bean asset.
89. Salicylic acid-mediated plant immunity and disease resistance under a warming climate
Supervisor: Christian Danve Castroverde
University: Wilfrid Laurier University (Waterloo campus)
We recently discovered that climate change-associated warm temperatures target various components of plant immunity, including the biosynthesis of the crucial defence hormone salicylic acid (SA). Our global transcriptome (RNA-Seq) analyses revealed that exogenous SA application in the model species Arabidopsis thaliana leads to both temperature-vulnerable and temperature-resilient sectors of gene expression. Our preliminary network analyses have shed light on candidate genes associated with these two gene clusters. However, detailed functional characterization of these genes is currently lacking. This Mitacs project will address this critical knowledge gap. In this project, we will characterize mutants for a subset of these temperature-resilient genes, including those that encode several ANAC and WRKY transcription factors. Specifically, we will infiltrate wild-type and mutant plant leaves with SA to induce immunity and defence gene expression. We will then infect both local and systemic leaves with the model bacterial pathogen Pseudomonas syringae pv. tomato (Pst) DC3000. Plant tissues will be collected to quantify bacterial growth in planta at both normal and elevated temperatures. Mutant plants with altered (higher or lower) bacterial levels in response to SA compared to wild-type plants will reveal which candidate genes centrally influence SA-mediated local and systemic immune responses. The results of this project will pinpoint which TF-encoding genes require further molecular, biochemical and physiological investigations in the future. Overall, we will unravel previously unknown layers of the genetic landscape regulating local and systemic immunity in a changing environment. This will also provide us with candidate genes for future genome editing as part of global efforts to enhance climate resilience of important agricultural crops.
Research area, student roles & skills
Research area: My research program is centred around understanding plant immune signalling and disease resistance in a changing environment. The overarching vision is to: (1) discover novel regulators of temperature-sensitive immunity; (2) determine if temperature-regulated immunity is conserved in diverse species; and (3) develop innovative technologies to repair the immune system and generate climate-smart plants. We integrate multiple approaches, from molecular biology and genetics to microbiology and biochemistry, to investigate the profound impact of changing temperatures on plant immunity and disease. It is anticipated that fundamental discoveries can be leveraged towards disease prevention and climate resilience programs in Canada and globally.
Student roles: The student will be involved in experimentation, data collection and data analyses. They will grow their own Arabidopsis plants and maintain Pst DC3000 bacterial cultures for SA protection assays. They will infiltrate plants with salicylic acid, inoculate them with pathogen and collect local and systemic samples. Tissue samples will be used for bacterial growth measurements by determining colony concentrations per sample. Experiments will be repeated for reproducibility.
Skills required: Required: Background in biology, basic lab experience Preferred (but not required): Advanced skills in molecular biology, microbiology and plant biology
Determining the sensory abilities of different animal species is crucial to understanding how they navigate their environments, find food and even interact with humans. Behavioural testing can, however, be costly, time consuming and difficult for many species. An alternative approach is to examine the anatomy of the sensory pathways in the brain responsible for detecting and discriminating among environmental stimuli. Using this approach, my lab has made several major discoveries in how birds perceive their environment. For example, turkey vultures have enormous olfactory systems that enable them to find dead animals before other scavengers arrive and hummingbirds have enlarged very specific visual nuclei in the brain that process how the direction and speed at which they move. These discoveries rely on sophisticated microscopy equipment in our lab as well as access to our extensive brain collection.
Currently our work relies primarily on our histology collection and microCT scanned material from museums around the world. We use these resources to gain a better understanding of how different bird species hear, see, and touch the world around them. This project is ideal for students interested in learning about brain and skull anatomy and the skills are transferable to medicine, veterinary medicine, biomedical sciences and scientific research.
Research area, student roles & skills
Research area: My lab's research addresses the broad question: how and why does brain anatomy vary across and within species? To answer this broad question, we have worked on a variety of different vertebrate species, but most of our research focuses on birds. Our lab hosts the world's largest bird brain collection and we have a global network of collaborators.
Student roles: The successful applicant will learn how to process a brain from dissection through histology and image analysis by the completion of the internship. Students will be involved in dissections, preparation of fixatives and other solutions, staining brain sections and analyzing the tissue with the aid of a high resolution slide scanner and/or an upright microscope equipped with specialized software. For students more interested in microCT scanning, the successful applicant will learn how to segment, reconstruct, and measure scanned specimens with specialized software as well as gain experience with dissections and most aspects of histology. All lab work is done alongside other students so that everyone can assist one another throughout training. The student will also be invited to participate in weekly lab meetings to discuss scientific papers and progress on the variety of research projects being undertaken as well as our annual lab trip to a national park, museum or zoo to learn more about the animals that we study and the life and culture of Indigenous peoples.
Skills required: The successful applicant should be undertaking a degree in biology, veterinary medicine, paleontology, or medicine and have some basic wet lab skills, such as using pipettes and electronic balances. Experience in dissections or histology is desirable, but not necessary. Note that it is unlikely that the research will involved fieldwork or animal handling.
91. Spatial immune profiling of prostate cancer bone metastases
Skeletal bone metastasis occurs in >90% of patients with metastatic prostate cancer (PCa), contributing to bone fractures, spinal cord compression, and debilitating pain. Despite the clear affinity of prostate cancer clearly for the bone, the interactions between bone, tumour and immune cells remain poorly understood. Further, it is unclear how the immune microenvironment differs between localised and metastatic prostate cancer stratified by molecular subtype. Through a collaboration with the Vancouver Spine Oncology Biobank, we are studying bone metastases from a unique surgical cohort of prostate cancer patients (n=18) providing blood, matched primary and surgically resected spine metastases.
The aims of this project are:
Determine the phenotype and spatial localisation of tumour and immune cells in bone metastases: A 15-marker multiplexed immunofluorescence (mIF) panel (Akoya Phenocycler) has been developed in-house to capture the phenotype and location of mPCa and immune cells in soft bone metastases. Spatial imaging analysis tools including QuPath, PathML and SPIAT (R packaged) will be used to measure cell densities and define cellular neighbourhoods with tumour core, periphery and stromal regions.
Infer spatial proteomic profiles in routine H&E slides using a machine learning model:
To link spatial biology to routine morphological assessment of mPCa, GigaTIME, a machine learning model will be trained on paired H&E and mIF slides of bone metastases. The model outputs photorealistic virtual mIF images by learning a cross modal translation between tissue morphology and protein expression. Model performance will be evaluated using a held out test set of H&E and mIF data using Pearson correlation and Dice score.
Outcomes: This work will define the immunogenomic landscape of prostate cancer bone metastases. Successful implementation of the machine learning model will enable inexpensive spatial profiling of bone metastatic PCa from routinely acquired pathology slides.
Research area, student roles & skills
Research area: Metastatic prostate cancer is lethal despite many available therapeutic options including genomic biomarker guided treatments. Recognising that tumours are complex ecosystems, my research program seeks to understand the interplay between the genetic dependencies of incurable human prostate tumours and the immune system. By integrating genomic profiling and spatial imaging, my team investigates how aggressive human prostate tumors evade immune detection. Our goal is to identify genetic and immune biomarkers that predict patient outcomes and responses to immunotherapy in metastatic prostate cancer.
Student roles: As a research student at the BC Cancer- Victoria’s Trev & Joyce Deeley Research Centre, you will play an integral role in advancing spatial biology research. Upon joining the lab, you will complete mandatory onboarding training in laboratory safety, research ethics, and occupational health protocols. Your primary responsibility will be the computational analysis of paired hematoxylin and eosin (H&E) and multiplex immunofluorescence (mIF) imaging datasets generated from bone metastasis specimens. You will assist in the quality control (mainly QuPath based), data organisation and implementation of analysis workflows using R and Python based tools (SPIAT, PathML) and machine learning frameworks (GigaTime).
Wet lab responsibilities are not required; your contributions will be exclusively computational. You will report your findings in regular one-on-one meetings with your supervisor and present updates at team meetings. Your work may contribute to lab publications, and your contributions will be acknowledged in authorship.
Participation in scientific activities is expected, including joining departmental journal clubs, and engaging in lab meetings. At the conclusion of your term, you will deliver a final presentation of research findings to the researchers at the Deeley Research Centre’s weekly group meeting. This role is an excellent opportunity to gain experience in spatial biology, data interpretation, and scientific communication in a highly collaborative research environment.
Skills required: Please describe the required skills/background of the student (100 words): Motivated to work within a multidisciplinary team of researchers and collaborators with backgrounds in immunology, genome science, clinical oncology and urology. A background in bioinformatics (proficiency in Python, R, bash), computational biology or related fields
92. Spatial modelling of animal movements
Supervisor: HAO WANG
University: University of Alberta (Edmonton campus)
Location: Edmonton, Alberta
Start date: 2027-05-03 (flexible)
Disciplines: Biological Sciences, Biology, Ecology, Engg-Biological, Environmental Studies, Forestry, Human Ecology, Mathematics, Microbiology, Physics, Science and Technology, Zoology
Animal movements and their underlying mechanisms are an extremely important research area in biology and have been extensively studied for centuries. To fully describe and understand animal movements is still challenging and needs substantial efforts in both empirical and theoretical studies. This research project is to study the spatial memory and cognition as well as social interactions of animals for mechanistic modeling of animal movements.
Research area, student roles & skills
Research area: Wang research group works on several areas of mathematical biology as diverse as modeling stoichiometry-based ecological interactions, microbiology, spatial ecology, infectious diseases, habitat destruction and biodiversity, risk assessment of oil sands pollution. Mathematical models include ODE, DDE, PDE, SDE, IDE.
Student roles: Developing or modifying mathematical models; numerical simulations; simple math analysis; writing a report
Salmonella enterica is a significant microbial contributor to food-borne illness causing over 1 million infections per year in the United States. S. enterica encodes Type 3 secretion systems (T3SS) that are essential for pathogenesis and transport bacterial effector proteins into host cells. These effector proteins interface with, and subvert host processes to favour the pathogen. The highly conserved “novel E3 ubiquitin ligase” (NEL) effector proteins (SspH1, SspH2, SlrP) have been reported to subvert host immunity.
Experiments in human cultured cells will focus on host protein interactions between SspH2 and host innate immune components. A candidate list of potential interactors will be investigated and alterations in immune signaling will be characterized as a consequence of potential interaction with SspH2. Experiments in yeast will focus on an in vivo yeast lethality screen that has been developed as a powerful selection for small molecule inhibitors of NELs. Chemical and genetic suppressors of the NEL-induced lethality phenotype will be pursued.
NEL inhibitors would be valuable probes for studying bacterial pathogenesis, and as potential anti-infectives, as these enzymes are proposed to primarily subvert immunity and have been identified in other globally important infectious bacteria including Yersinia, Escherichia, Pseudomonas and Shigella.
Research area, student roles & skills
Research area: One of the Bhavsar laboratory’s specialized area of research is in bacterial pathogenesis. We strive to understand the mechanistic details underlying interactions of bacterial effector proteins and host factors. We are particularly interested in the Novel E3 ubiquitin ligase (NEL) effector proteins. Using a variety of model systems including cell culture, yeast and plants, we aim to discover new host-pathogen interactions and leverage these insights to target bacterial pathogenicity and explore anti-infective strategies. The goal of the research program is to understand the different strategies bacterial pathogens employ in a range of hosts that potentially spans kingdoms.
Student roles: Non-laboratory roles: The student will be required to read relevant background literature and experimental protocols. A written report of the research project will be expected at the end of the 12-week period that will remain with the principal investigator. In addition, the student will be responsible for recording any new protocols developed in the course of their project. The student will be required to create a LinkedIn/Researchgate profile that includes the laboratory in their network.
Laboratory roles: The student will work in the lab under partial supervision of a senior graduate student or the principal investigator. Depending on their background, the student will be expected to conduct experiments related to tissue culture, yeast transformation and growth or plant agro-infiltration. In addition to performing experiments, after appropriate training, the student will be expected to perform data analysis to be presented to their supervisor and/or at lab meetings. The student will also be assigned routine lab “jobs”, particularly those pertaining to the equipment/resources used in their project.
Skills required: The student should have a background in biological sciences and be familiar with microbiology, molecular biology and biochemical concepts. Experience with tissue culture, yeast, and/or plant molecular methods would be an asset. The student must be an effective communicator and well-organized. The student should be comfortable with, and have the ability to, work largely independently after initial training. A high degree of critical thinking skills is expected.
94. Synthetic Indoor ecosystems for recyling and food production
Supervisor: Jurek Kolasa
University: McMaster University (Hamilton campus)
Location: Hamilton, Ontario
Start date: 2027-05-03 (flexible)
Disciplines: Biological Sciences, Biology, Ecology, Agriculture, Engg-Biological, Human Ecology, Zoology
The close integration of city dwellers with growing and recycling food nurtures sustainability. To advance
this, we integrate biodiversity and technology as Synthetic Ecosystems (SE) to grow food on recycled food
waste at home and to fit with human lifestyles. Our main goal is to couple the power of biodiversity and
technology adaptable for use in diverse social settings. Biodiversity has the ability to make SE service
productive, stable, and resilient via compensatory, mutualistic (species complement each other) and
regulatory functions (feedback controls). The key issue is that biodiversity’s complexity and variability
hinder its use. The exciting challenge of this venture is to harness biodiversity (multiple species of
different functions in the production line) so it behaves in ways we want it to. Specifically, we want the
nutrients recovered from green waste to be released efficiently (digestion), converted to green biomass
(algae, hydroponic plants), and fed to invertebrates (zooplankton and worms) and serve as food for fish.
All these processes must be balanced relative to one another, so an SE works predictably and for a long
time. For this, we study which factors undermine the balance, and procedures to prevent unwanted
variability. SEs will overcome this barrier by balancing functional modules. For this, we need to monitor
biological and physical parameters in various modules, and design actions that steady their performance.
To do so, we plan to install a variety of nanosensors and an Artificial Intelligence module (ecosystem
brain), which will accelerate slow and put brakes on too fast biological processes – a new and futuristic
technology.
Research area, student roles & skills
Research area: Synthetic ecosystems aim to provide specific services: The concept includes food waste digester,
phytoplankton, zooplankton cultures, and larger consumers (crayfish, prawns). Related to aquaponics, the
project employs and manages biodiversity within separate modules to make food. Such management is a
new research frontier. This interdisciplinary project integrates the ideas and technologies from vertical
agriculture, aquaponics, and ecological engineering to supplement food production in cities. Specific
knowledge required for its success includes population dynamics, species interactions, nutrient cycles,
ecological energetics, and tools for monitoring the state of the system (sensors, monitoring, analysis).
Student roles: The student(s) will take responsibility and a substantial role in: - one of the crucial aspects of the experimental work (this will be assigned in response to student interests and ability - in problem-solving, testing new ideas for improving operations and maintenance of biodiversity constituents of the synthetic ecosystem. This is a research project that input from all personnel engaged is desirable and important assisting with the routine operation of experimental systems - tracking and reviewing most recent developments (monitoring professional meetings, news releases, and literature) and submitting a final report on what is the cutting-edge state in developing integrated and compact food production systems, or what are the main obstacles to success. The students will also participate in group meetings and be given a full voice. We will further encourage students to suggest their own ideas for the project improvement make it possible for them to present these ideas more formally in a devoted session.
Skills required: Desirable: some courses (or passionate interests) in one or several of Agriculture (especially vertical), Ecology, Limnology, Aquatic Biology, environmental engineering; familiarity with Excel, basic chemistry, system modeling
95. Temporal Synchrony and Ecological Change in Great Plains Lake Ecosystems
Freshwater lakes are closely connected to the landscapes around them. Regional climate, watershed features, and local environmental conditions influence how water, nutrients, and energy move through lake ecosystems. These processes shape water chemistry, food availability, habitat conditions, and the structure of biological communities. However, lakes do not always respond to environmental change in the same way. Some lakes show similar patterns over time because they are influenced by shared regional drivers such as climate, while others respond more independently due to local watershed conditions or ecological interactions.
This project will explore how lakes across the Great Plains region of Alberta respond to environmental variability through time. The student will examine the concept of temporal synchrony, which describes whether ecological variables fluctuate in similar ways across multiple lakes. Strong synchrony may indicate the influence of broad regional drivers, whereas weak synchrony may suggest that local factors, such as watershed land use, lake morphology, nutrient status, or biological interactions, play a stronger role.
The project will use 20-year ecological time-series data from approximately 75 lakes across the region. Datasets will include water-quality variables such as nutrients, salinity, pH, alkalinity, water clarity, dissolved oxygen, and temperature, as well as phytoplankton community composition and diversity. Climate data will be incorporated alongside watershed-scale variables describing hydrology, land cover and land use, and lake morphometry.
The student will gain hands-on experience compiling and analyzing long-term ecological datasets, conducting literature reviews, and applying statistical tools in R. Temporal synchrony among lakes will be evaluated using appropriate quantitative approaches, and additional analyses will examine how regional climate and local environmental characteristics influence lake ecosystem dynamics. This project will provide valuable training in freshwater ecology, ecological statistics, and long-term environmental monitoring, while contributing to a broader understanding of how prairie lake ecosystems respond to climate variability and environmental change.
Research area, student roles & skills
Research area: My research integrates aquatic ecology and quantitative ecology, with a focus on understanding and predicting the cumulative effects of human activities on freshwater ecosystems. I use quantitative approaches to explore ecological patterns and processes across multiple spatial scales, from local to continental, and across a range of organisms, from microbes to fish. I am committed to advancing data-driven, policy-relevant science to inform sustainable freshwater resource management.
Student roles: The student will play an integral role in this project, contributing to key aspects of data preparation, analysis, and interpretation. They will compile and prepare data from multiple sources, including lake survey datasets and land use/land cover information, and conduct a literature review to inform the project’s conceptual framework and support interpretation of results.
A major component of the role will involve learning and applying quantitative methods to examine how lake ecosystems respond to environmental variability through time. The student will use R for data analysis and gain valuable experience working with large ecological datasets.
As a supervisor, I am committed to providing a supportive and collaborative learning environment. I will offer regular one-on-one mentorship, guiding the student through all stages of the project from data compilation and analysis to interpretation and scientific writing. The student will also have opportunities to engage with my broader research network and contribute to publications resulting from the project.
Skills required: The ideal student should have a background in ecology, environmental science, biology, statistics or a related field, with an interest in aquatic ecosystems and quantitative modeling. Strong analytical skills and experience with statistical analysis (preferably in R) are desirable. Familiarity with GIS and spatial data analysis is an asset but not required. The student should be comfortable working with large datasets and have strong written and verbal communication skills. Curiosity, initiative, and the ability to work both independently and collaboratively are essential for success in this project.
96. The Impacts of Ocean Literacy Outreach in Rural Nova Scotia
Supervisor: Regina Cozzi
University: St. Francis Xavier University (Antigonish campus)
This long-term project aims to enhance ocean literacy in youth in Nova Scotia. Qualitative and quantitative data will be collected and analyzed to evaluate the program, its efficacy, and impact in rural communities.
Nova Scotia is a small coastal province whose culture and economy is inextricably intertwined with the marine environment. Despite being surrounded by the ocean and a strong dependency on ocean resources, research shows that our youth demonstrate a lack of knowledge about ocean concepts. In fact, the Nova Scotia classroom science curriculum focus on ocean sciences is limited to grades 10 to 12, with minimal inter-disciplinary connections between Biology, Chemistry, Earth Science, Engineering, Math, Physics, Nutrition, etc. While many educators value ocean literacy, they are challenged with a lack of resources, face time constraints and curriculum restrictions to successfully integrate ocean content in their classrooms. The X-Oceans Outreach mobile program is trying to bridge this gap in the education system. We travel by means of an outreach van to bring our programming and live marine organisms to rural communities. Our ocean-based hands-on STEM activities provide youth with opportunities to explore the oceans and their importance in the climate change battle.
This position will enable you to hone key valuable transferable skills, including soft skills (e.g., communications, leadership, critical thinking, problem solving, etc.) and technical skills (e.g., research literature, project management, data collection, data analyses, etc.) that will benefit you in any future career.
Research area, student roles & skills
Research area: This research focuses on the impacts of the X-Oceans Outreach mobile program on enhancing ocean literacy in underserved rural communities of Nova Scotia. The program promotes ocean stewardship by providing ocean education to youth (ages 6-18), through Science, Technology, Engineering and Mathematics (STEM). Ocean-based hands-on activities give youth the opportunity to explore the importance of marine biodiversity and ocean-related careers, with focus on preservation and climate change mitigation. Outreach workshops include touch-tanks housing live marine organisms (e.g., sea cucumbers, sea anemones, sea stars, crabs, etc.) and active learning tools (e.g., ocean-centered STEM activities and experiments) to enhance experiential learning.
Student roles: Student duties and responsibilities will consist of: • Collecting, organizing and analyzing quantitative and qualitative outreach data • Analyzing survey responses • Transcribing information • Report writing • Revising and improving ocean education content for youth • Helping with on-campus and off-campus outreach activities • Debriefing with supervisor and team members
Skills required: Required skills include: • Excellent organization skills • Excellent time management and punctuality • Excellent adaptability skills • Strong work ethic, hardworking • Knowledge in STEM, ocean related sciences and marine biodiversity • Detail oriented • Ability to work independently and in a team • Excellent communication and writing skills • Computer skills (Word, Excel, PowerPoint, etc.) • Positive attitude, reliable and dependable • French language is an asset • This position requires working with youth; therefore, a Child Abuse Registry check and a Vulnerable Sector Check is mandatory.
97. The Invisible Injury: Understanding the Mechanisms behind Mild Traumatic Brain Injury
Supervisor: Brian Christie
University: University of Victoria
Location: Victoria, British Columbia
Start date: 2027-05-03 (flexible)
Disciplines: Biological Sciences, Biochemistry, Medical Sciences, Neuroscience, Anatomy
Concussions are a form of mild traumatic brain injury (mTBI), often called the “unseen injury”, because of the lack of obvious damage. As a result, many individuals suffer repeated concussions, often in short time spans. In some sports, like hockey and football, awareness is growing that repeated sub-concussive blows to the head are routinely incurred in practices and games. Although many of these impacts do not result in a diagnosis of concussion, there is a growing appreciation that both concussions and sub-concussive impacts can negatively impact cognitive function in the developing brain, and that repeated concussions (r-mTBI) can have long-term detrimental effects for cognition in elderly populations. Despite these concerns, few laboratories have systematically studied r-mTBI in a preclinical model that properly mimics the human condition. We have recently developed a model that enables us to accurately examine r-mTBI and understand how the brain responds to repeated mTBI. Because significant structural damage does not occur in mTBI, our focus is on whether there is damage to small blood vessels in the brain. These vessels are below the threshold of resolution for magnetic resonance imaging, but can be resolved using high resolution microscopy. When damaged, these vessels can release a compound in the blood called fibrinogen. Fibrinogen is normally important for clotting, but can also initiate an inflammatory process involving brain microglia that negatively impacts neurons, and impairs learning and memory processes. The results of the proposed work will help us to better understand the mechanisms involved in mTBI; how they directly impact brain function, and increase our understanding of how r-mTBI early in life could negatively impact individuals as they age. More importantly, this work will enable us to determine the therapeutic value of modulating fibrinogen’s effects in the brain to help reduce the negative impact of r-mTBI on cognition.
Research area, student roles & skills
Research area: We study a variety of forms of neuroplasticity, including synaptic plasticity, metaplasticity, and neurogenesis, to better understand both the intrinsic processing capacities of the brain, how it responds to injury, and it’s capacity for self-repair.
Trainees in the laboratory have the ability to work with animal models that include those for acquired brain injuryto better understand how learning and memory processes are impacted, and examine different interventions for their therapeutic potential. Our laboratory places a strong emphasis on trainee development, and supports equity and diversity initiatives both in the lab and in trainee activities outside of the lab.
Student roles: The student will assist either a post-doctoral fellow or a graduate student by conducting an independent project during their time in the laboratory. Students should be self-motivated and will be provided with instruction and the support needed to complete the project.
Skills required: Basic biochemistry/biology/histology skills are preferred. Individuals with microscopy, biochemistry, or immunohistochemistry experience are encouraged to apply.
98. Theoretical basis of embodied consciousness in robots
The research project is an open ended exploration of what is it like to be a conscious robot from multiple perspectives. An emerging framework for our laboratory involves formulating models based on Dynamic Field Theory that can be implemented in spiking neural networks. We are however mostly interested in setting the initial theoretical backgrounds to develop the hypotheses that can then be empirically tested. Therefore, this initial phase of the project is exploratory and theoretical although it may require simulation observations and small experiments.
Research area, student roles & skills
Research area: The area is multidisciplinary between philosophy, neuroscience and robotics with various aspects of computer science and engineering.
Student roles: Extensive reading and analysis of text is required. Writing and communication skills must be top. Quantitative skills are a must but must be able to speak to people who don't have them in lay person language. Anility to work in a multidisciplinary diverse and international team.
Skills required: Interested students need to be interested in Automata Theory, Deep learning theory, computational models and embodied robotic cognition/emotion. Students must have a tolerance to uncharted territory, risk and uncertain outcomes.
99. Towards understanding the role of kinetochore aberrations in breast cancer progression
Kinetochore Project description:
Breast Cancer (BC) is a highly heterogenous disease with some cases being associated with slow growth and excellent prognosis whilst other tumors exhibit a highly aggressive clinical course. Using a cell culture model of breast cancer progression, we have identified using genomic approaches and cell biology approaches that cells from a BC ‘continuum’ series that mimics the different stages of cancer progression exhibit a stage-dependent increase in structural and numerical centrosome defects. In human cells, centrosomes form the poles of the mitotic spindle which is responsible for binding to and segregating chromosomes. Consequently, dividing cells in this series exhibit chromosome segregation defects that increase with disease progression. Using genomic methods, we have found that some of the most effected cell cycle genes are those implicated in kinetochore maintenance. In this project, we will use cell biology and molecular biology techniques to determine the contribution of kinetochore defects to BC progression. Three aims are envisioned to test this hypothesis:
AIM1: To detail mitotic defects leading to chromosome missegregation in the BC continuum model. We will use high resolution microscopy, live-cell imaging and image analysis to observe, quantify and catalog the mitotic defects in the BC series.
AIM2: To characterize kinetochore defects in the BC continuum and correlate them with disease stage using similar approaches as aim1.
AIM3: Identify changes in expression of mitotic genes from the BC series human transcriptome arrays (HTA) and correlate to the identified mitotic defects. Our collaborators have generated genome-wide transcriptome of the different stages of BC from the continuum series as well as patient samples. We will use this dataset to identify mitotic genes that are implicated in BC initiation and progression.
All the assays are established in the Elowe lab and all necessary equipment and reagents are readily available.
Research area, student roles & skills
Research area: Our lab is interested in the molecular mechanisms of cell division. When a cell divides, it should do so equally, with 'daughter' cells receiving equal amounts of chromosomes from the original 'mother' cell. If this does not happen, the resulting daughter cells will have unequal number of chromosomes, which is detrimental to the health of daughter cells and can cause developmental defects and cancer. Our lab studies how signalling mechanisms in dividing cells ensure that chromosomes are equally segregated into daughter cells, with an emphasis on the role of specialized subcellular structures such as the mitotic spindle and centrosomes.
Student roles: Overall, the student will be implicated in all stages of the experimental process. For this project, the student will be mainly involved in aims 1 and 2 listed above, and depending on interest/time, may also assist in the transcriptome analysis for aim 3. The student will be directly supervised by a senior graduate student/post-doc and the lab head in the beginning, but as the project progresses, the student will take a more independent role in driving the project, including but not limited to experimental design and data analysis. In terms of techniques, the student will be trained and will eventually independently design and perform immunocytochemistry (immunofluorescence) experiments as well as live-tracking of cells undergoing cell division. The recruited student will acquire in-depth experience of spinning-disc confocal microscopy, image analysis (using image J/FIJI ) and quantitative cell biology. As the student becomes more experienced, there will be more autonomy and opportunity to explore alternative approaches and hypotheses. In addition, the student will be involved in the analysis of the data directly with the main supervisor, and will be involved in the presentation of the final dataset in a scientifically acceptable form (e.g. graph, figure panel etc.). There will also be opportunities for presentation of the results of the project in a poster or short talk format at a local symposium, which is encouraged.
Skills required: - Good communication skills. - Enthusiasm, ambition, and interest in the project. - Willingness to work in a team-environment. - Previous laboratory experience is an asset but not necessary.
100. Toxic protein aggregates are drivers of neurodegeneration and other diseases
Recent breakthroughs in cell biology revolutionized the science and research of neurodegeneration. Thus, it became clear that the transport of macromolecules in and out of the cell nucleus is critical for the formation of toxic aggregates that are linked to neurodegeneration. Prominent examples of such disorders are Alzheimer's disease (AD), Huntington's disease, amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD).
While nuclear transport is essential for the onset and progression of neurodegeneration, the molecular players and their contribution to disease are not well understood. It is the goal of our studies to define these processes and identify new potential drug targets. Long-term, our research project will help to develop new therapeutic strategies for neurodegenerative and other diseases that are caused by toxic aggregates.
Research area, student roles & skills
Research area: Our laboratory studies the impact of aging, stress, or metabolism on human health. These factors contribute to the onset and progression of some of the most debilitating diseases. This includes many forms of neurodegeneration. Neurodegeneration is often characterized by the formation of toxic protein aggregates. Little is known about the biogenesis of such aggregates. Our group investigates these questions with modern methods in physiology, cell biology and biochemistry. We have special expertise in state-of-the-art imaging, high throughput screening and other cutting-edge technologies. The current project is a new and exciting approach to examine the formation of toxic cellular aggregates.
Student roles: The student will be involved in all aspects of the project. Together with the supervisor and other members of our group, the candidate will plan and optimize the research strategy, conduct experiments and trouble-shoot, if necessary. The student will actively participate in the interpretation of results and will keep appropriate records of all experiments. The candidate will discuss her/his data at lab meetings and present results at local scientific conferences. The student will work closely with other lab members and collaborators. The candidate will have the opportunity to attend scientific seminars and research meetings.
The student will perform and participate in the following activities: (1) Measure nuclear transport and the production of toxic aggregates in cultured cells. (2) Use pharmacological drugs to prevent or reverse the formation of toxic aggregates. (3) Measure how pharmacological drugs change the survival of aggregate forming cells. (4) Participate in the data analysis and interpretation. (5) Based on the candidate’s results, he/she will contribute to the writing of a scientific paper.
Throughout the project, the student will acquire new scientific methods and use state-of-the-art instrumentation. This includes, but is not limited to, advanced high resolution microscopy, quantitative imaging, metabolomics and other modern methods in cell biology, biochemistry and physiology.
Skills required: The ideal candidate will have a good theoretical background in cell biology, physiology or related fields. Enthusiasm, scientific curiosity and the ability to work in an international team are essential. To this end, the student can communicate in English, both orally and in writing. Previous research experience at the bench is an asset, but not mandatory.
101. Turing’s instability mathematical model applied for Harmful Algae Blooms (HAB) and the ecological scale issue
This project will focus on the Turing’s instability with advection-diffusion terms into our deterministic models for Harmful Algal Blooms (HAB) using the scaling technique (dimensionalization) for global scale predictions of HAB. Methods: Turing’s mechanism is fundamentally a diffusion instability that is considered an important process that causes plankton patchiness in ecosystems. Turing’s instability occurs when the diffusion coefficient of the inhibitor (predator-plankton) is larger than that of the activator agent (prey-nutrient or heat) in a dynamic system, causing spatially uniform distributions of species to become unstable. The system will settle into a stationary state, leading to the emergence of HAB patterns on the surface of water when the diffusivity of algae is quicker than nutrients. Differential flow instability, defined as ‘advection’ effects between two constituent species, arises naturally in the shear flow can lead to spatial differentiation in plankton communities. “Advective blooms” are defined as those in which the variability in a time-series of algal biomass is dominated by spatial transport of existing biomass rather than biological growth and loss processes. Advective processes can act both vertically and horizontally. Lateral advective processes which can concentrate algal biomass in one part of a water body due to currents will be considered as the spatial distribution of algae is driven by this additive convection. Expected results: This theme provides the concept of couple biological/physical processes at the scale of algal organisms to predict larger scale plankton and fluid dynamics. Our results will reveal the interaction between plankton demography and motion through their effect on implications for natural plankton populations.
Research area, student roles & skills
Research area: Dr. Nguyen-Quang's research centers on advancing Aquatic Science as a cornerstone for sustainable environmental solutions, particularly in the face of climate change and anthropogenic pressures on coastal and freshwater ecosystems. Through an interdisciplinary lens, he investigates the complex interactions between coastal hydrodynamics, surface water quality, and ecology, including cyanobacteria in eutrophic systems. His work integrates computational biosystems modeling, biophysical process analysis, and mathematical frameworks to decode natural patterns and assess biomass dynamics. To address these challenges, he uses state-of-the-art approaches such as remote sensing, hydrodynamic simulations, and AI-machine learning, bridging data-driven insights with actionable strategies for environmental management.
Student roles: The required work during 12 consecutive weeks will be concentrated on:
• Data collection for validation of mathematical model and programming (the core task), • Integration of weather data and the exploitation all related information for predicting purposes. • Participation in the design of the user interface (with a focus on logged data viewer and implementation of decision support components) • The follow-up tasks will be carried out in parallel with necessary redesigns/adjustments of the subsystems defined above, in close collaboration with other trainees in the project • Interpretation of results • Report writing
Benefits Student will benefit from working in BBML with Dr.Tri Nguyen-Quang in a various manners: • Experience in applying technical procedures and practices; • Exposure to the management, prediction and monitoring of eutrophic lakes and rivers for water uses, especially for drinking water resources; • Develop and enhance interpersonal skills needed to succeed in a professional career; • With four graduate students (3 PhD and 1 Master) and one research assistant currently working in BBML, the successful candidates will be integrated in a High quality of academic research team within an internationally enthusiastic and friendly atmosphere. • The project, if well done, will be subject to a Master or PhD research level in the future depending on the funding opportunities.
Skills required: It is expected that the candidates with a strong background in Mathematics and Programming from Environmental, Biological and Ecological concepts are targeted.
Moreover, they are expected to be strong in oriented tools like MATLAB/Mathematica or Python, and in programming languages suc as C++ or Fortran. The successful candidates must be a motivated person who work well in a team environment. Students must exhibit excellent written and oral communication skills (either in French or in English).
102. Ultrasensitive biosensing with advanced microscopy
Decades of over-administering antibiotics have led to widespread drug resistance in pathogens, increasingly rendering these life-saving therapeutics ineffective. By 2050, it is estimated that hundreds of millions of people will be affected by antimicrobial resistance (AMR) each year. It is, therefore, increasingly urgent to ensure the efficacy of therapeutics before they are prescribed to patients.
In a standard antimicrobial resistance, bacteria are cultured in the presence of antibiotics while the growth rate is monitored over many division cycles. While robust, this takes many hours. A faster and low-cost solution is to infer susceptibility by measuring how specific physical properties of cells are affected by antibiotics. Signatures of drug susceptibility (and resistance) can be chosen for reliability and usefulness. Of particular interest is the remarkable propensity of live microbes to attach to surfaces, i.e. surface fouling.
This project explores a new type of assay that infers drug-susceptibility by characterizing the capacity bacteria have for attaching to surfaces. Specifically, we propose optically monitoring the nanoscale junction between cells and functionalized surfaces using a nearfield optical assay. The sensitivity of our approach is derived from the localized measurement of the refractive index, which is targeted to the area under a bacterium – in our approach, this is done by exploiting an interfacial optical effect known as supercritical-angle fluorescence.
Research area, student roles & skills
Research area: The Weiss lab develops imaging-based approaches for a wide variety of biomedical applications. This includes new types of rapid-scanning microscopes for cancer diagnosis, ultrasensitive bacteria-characterization devices, and signal-analysis tools to make sense of multidimensional and noisy datasets.
Our multidisciplinary team comes from a variety of areas including biomedical engineering, chemistry, materials science, physics, and more. Together with collaborators, we are deploying our knowledge of optics, quantitative analysis, biophysics, and chemistry to engineer solutions to challenging problems.
Student roles: The specific objectives of this project are to develop and incorporate a fluorescent nanofilm into a microfluidic device for biosensing applications. You will lead an aspect of this project by performing a literature review to identify possible solutions. You will then summarize the previous work done in the area and propose possible solutions. You will then implement one or more of your ideas and begin a validation study based on synthetic samples. This will involve developing a quantitative analysis pipeline for recording and assessing data to test the robustness, sensitivity, and other key parameters. Finally, you will propose improvements to the design and identify potential further applications. At all stages of the project, you will participate in the team meetings with group members, and meet regularly with Professor Weiss as well as collaborators. For these meetings, you will summarize your results in brief presentations and discuss possible directions for your research. Throughout the program, there will be various opportunities to attend professional research events and present your research, for example as a poster.
Skills required: To succeed in this project, you must have experience with optical systems. This could be theoretical background, hands-on experience, or both. Experience with signal processing and image analysis will be necessary to process data quantitatively. At least some knowledge or experience in one of the following programming languages will be needed: Python, Matlab, Java, C++.
103. Understanding the effects of RAB32 mutations using human stem cell-derived microglia
Supervisor: Jay Penney
University: University of Prince Edward Island (Charlottetown campus)
Parkinson’s disease is a progressive neurodegenerative condition characterized by the loss of dopamine producing neurons from the substantia nigra region of the brain. While the exact causes remain unknown, genetic and functional studies have demonstrated that non-neuronal brain cell types, in particular microglia, can make key contributions to neuron degeneration in Parkinson’s disease and related conditions.
The S71R amino acid substitution in the small GTPase RAB32 was recently identified as a cause of familial Parkinson’s disease. While RAB32 is highly expressed by microglia, the effects of S71R and loss-of-function RAB32 mutations on microglia have not yet been examined. This project will explore their impacts using human induced pluripotent stem cell (iPSC) models. CRISPR/Cas9 mutagenesis has been performed by members of my lab to generate iPSC lines carrying RAB32-S71R and RAB32-Null mutations. For this project, control and mutant RAB32 iPSCs will be differentiated using well-established protocols into iPSC-derived microglia, followed by characterization of basic microglia functions.
Microglia are the innate immune cells of the brain, functioning to sense and remove protein aggregates and other debris while also being the main mediators of brain inflammation. We will assess microglial phenotypes using techniques including immunostaining, fluorescence microscopy, western blotting, flow cytometry and ELISA (enzyme-linked immunosorbent assay) in order to test whether RAB32 mutations affect microglia viability, cellular motility, phagocytic capacity and inflammatory responses, as well as key cell signaling pathways. This project will be among the first examinations of RAB32 mutation effects on microglial function in any species and will provide insight into the normal and pathogenic roles that this protein can play in the brain.
Research area, student roles & skills
Research area: Genetic and functional studies have revealed key roles for microglia, the brain’s innate immune cells, in the development of various neurodegenerative diseases. The overarching goal of my research program as Canada Research Chair in Biomedical Genetics is to push forward our understanding of microglial contributions to the development and progression of neurodegeneration. This will be achieved by leveraging gene editing techniques and human induced pluripotent stem cell (iPSC) models to examine the molecular and cellular effects of disease-causing mutations.
Student roles: The overall project will involve: 1) maintenance and growth of induced pluripotent stem cells (iPSCs), 2) differentiation of iPSCs into microglia, and 3) functional characterization of iPSC-derived microglia. The primary required role of the student will be the functional characterization of control and RAB32 mutant iPSC-derived microglia.
Functional characterization of iPSC-microglia will involve the use of immunostaining and imaging techniques, as well as flow cytometry, enzyme-linked immunosorbent assay (ELISA) and western blotting to assess the effects of RAB32 mutations on microglial morphology, motility, phagocytic capacity and inflammatory responses. The student will work alongside Dr. Penney and/or lab members to learn the necessary techniques and procedures to carry out the planned experiments. The student will also be guided in the design of scientific experiments, data collection and analysis, and will have the opportunity to discuss and present their experiments and findings.
The student will further gain training and experience in iPSC culture and differentiation techniques; however, these procedures are both time-consuming and advanced for someone without prior experience. As such, full time members of the laboratory will have microglia cultures prepared for the prospective student to use during the early stages of the project. This will allow the student to begin functional characterization experiments at the outset of the internship while learning iPSC culture and differentiation techniques in parallel.
Skills required: The student should have prior experience with sterile technique and some form of cell culture. Experience in induced pluripotent stem cell (iPSC) culture would be an asset but is not necessary. Some form of prior laboratory-based research experience is also preferred. In addition, background knowledge and understanding in areas including neuroscience, genetics, cell biology, and/or molecular biology would also be assets but not essential.
104. Using cancer metabolism to increase immunotherapy efficacy against solid tumours
Supervisor: Yannick Audet-Delage
University: Université du Québec à Trois–Rivières
Location: Trois-Rivières, Québec
Start date: 2027-05-03 (flexible)
Disciplines: Biological Sciences, Biology, Biochemistry, Engg-Biomedical, Health Studies, Immunology, Medical Sciences, Medicine, Molecular Biology, Pharmacology, Physiology, Science and Technology
Tumour metabolism is a recognized hallmark of cancer. Indeed, cancer cells increase cellular fluxes to promote proliferation and hijack metabolic pathways to support therapy resistance. However, metabolic remodeling is not exclusive to cancer cells: immune cells also change their metabolic activity to support immune functions. Notably, macrophages increase their glycolytic activity to maintain pro-inflammatory activity, whereas an oxidative profile promotes anti-inflammatory activity.
While melanoma is the deadliest skin cancer worldwide, breast cancer (BrCa) remains the second cause of death. Lately, immunotherapy has greatly improved the survival of melanoma and triple-negative BrCa patient. However, almost half of patients do not respond favorably to immune checkpoint inhibitors. While tumour glycolytic activity promotes an anti-inflammatory profile in tumour-associated macrophages (TAMs), elevated presence of TAMs is nonetheless associated with better response to immunotherapy. However, it is unclear how the metabolism of tumour and TAMs synergize in relation with response to immunotherapy. We thus hypothesize that altering the metabolic activity of tumours and TAMs could increase immunotherapy efficacy.
The project will involve two solid tumours: melanoma and BrCa. The aims of the project will be 1) to characterize the sensitivity of tumour cells to a panel of metabolic inhibitors targeting glycolysis, oxidative phosphorylation, glutaminolysis, etc.; 2) to characterize the effects of these metabolic inhibitors on the secretion by tumour cells of immunomodulatory molecules (cytokines, chemokines, etc.); and 3) measure the release of immunomodulatory molecules in coculture models.
The project will allow the trainees to develop skills in cell culture, pharmacological approaches, quantification of cytokines by ELISA, and diverse molecular and cellular biology techniques (qPCR, western blot, cellular assays, etc.).
At terms, results form this project will provide better understanding of the metabolic interactions between tumour and immune cells, and provide new therapeutic targets to improve immunotherapy efficacy against solid tumours.
Research area, student roles & skills
Research area: Cancer is a leading cause of death worldwide. To improve the life expectancy of patients, our laboratory uses multiomics approaches to uncover new therapeutic targets against solid tumours such as breast and melanoma. We have a particular focus on how metabolic adaptations support the development of resistance and how we can harness these changes to improve therapy response. We use several cutting-edge approaches to dissect oncometabolism in this optics, including extracellular flux analysis (Seahorse platform), high-content imaging, bioinformatics, transcriptomics, metabolomics, as well as diverse cellular and molecular biology techniques.
Student roles: The student’s role in this project would be to actively participate in the experimental and analytical components under the supervision of the research team. Typical responsibilities would include: • Cell culture work, including maintenance of tumour cell lines and participation in coculture models with immune cells • Execution of pharmacological experiments, testing metabolic inhibitors targeting pathways such as glycolysis or oxidative phosphorylation • Assessment of tumour and immune responses, using assays such as cell viability tests and quantification of secreted immunomodulatory molecules (e.g. cytokines, chemokines) by ELISA • Application of molecular and cellular biology techniques, including qPCR, western blotting, and basic functional assays • Data collection, analysis, and interpretation, with guidance from supervisors • Reading and discussing scientific literature relevant to tumour metabolism, immunology, and immunotherapy • Documentation and presentation of results, potentially contributing to reports, presentations, or posters Overall, the student would gain hands-on research experience, develop technical and analytical skills, and contribute meaningfully to understanding metabolic interactions between tumour and immune cells in the context of immunotherapy.
Skills required: Relevant skills and experience • Experience (or practical coursework) in cell culture • Familiarity with techniques such as ELISA, qPCR, western blotting, and cell viability assays • Interest in tumour–immune system interactions • Scientific rigor, critical thinking, and ability to work effectively in a laboratory setting • Comfort reading and understanding scientific literature in English Overall profile This project is particularly well suited for a student who is: • motivated by translational or fundamental research, • considering pursuing graduate studies (Master’s or PhD), • seeking to acquire strong experience in cellular, molecular, and immunological biology applied to cancer.
105. Validating a Novel Deep Learning Pipeline for Transposable Element RNA-seq Analysis
Transposable elements (TE) are mobile DNA sequences which have the ability to translocate to new locations throughout the genome. For some classes of TEs, translocation occurs through the expression of an RNA intermediate molecule which is reverse transcribed into DNA and inserted into a new location, generating many copies of the same sequence. Once thought to be largely repressed in humans TEs are actively expressed and mobile in the human brain particularly during brain development. Dysregulation of this activity has been implicated in several diseases including some types of cancers, schizophrenia and bi-polar disorder. However, due to their repetitive nature and high rates of polymorphisms, TE expression is difficult to study. Furthermore, most human RNA-seq analysis relies on mapping to the hg38 reference genome, which has incomplete resolution of TE elements.
This project aims to validate a novel pipeline for RNA-seq analysis of TEs. This pipeline incorporates more complete TE annotations derived from the telomere to telomere (T2T) genome and a deep learning model which assigns reads to specific loci based on the expression context of flanking regions. Validation will utilize simulated RNA-seq reads to provide a ground truth against which the pipelines performance can be measured
Research area, student roles & skills
Research area: My expertise is Bioinformatics and Machine Learning. My current research focuses on multi-omics analysis which provides novel information on the mechanisms of the biological process and cell states in disease development. We develop computational tools for complex and high-dimensional data including genome-wide population data, RNA-seq, and tandem mass spectra. I'm particularly interested in applying deep learning and AI to plants to advance digital agriculture.
Student roles: 1. Implement and evaluate the novel RNA-seq analysis pipeline incorporating T2T genome annotations for improved transposable element resolution 2. Simulate RNA-seq reads to generate ground truth datasets for benchmarking and validating pipeline performance 3. Integrate and apply the deep learning model designed to assign multi-mapping reads to specific TE loci based on flanking sequence context 4. Compare pipeline performance against existing TE analysis tools using standardized metrics of accuracy and resolution 5. Address challenges associated with repetitive sequence mapping, TE polymorphisms, and incomplete annotations in the hg38 reference genome 6. Optimize pipeline parameters and workflows to improve sensitivity and specificity of loci-specific TE expression quantification 7. Document findings through regular progress reports and lab meetings, and contribute to manuscript and figure preparation 8. Collaborate with supervisors and team members to refine the pipeline and integrate results within the broader context of TE biology and genomic medicine
Skills required: 1. Strong foundation in molecular biology and genomics, including an understanding of DNA/RNA biology, transcription, and mobile genetic elements 2. Proficiency in bioinformatics and RNA-seq data analysis, including familiarity with alignment tools, transcriptomic pipelines, and expression quantification methods 3. Computational skills including command-line (Linux/bash) and scripting experience in Python or R for pipeline development, data processing, and visualization 4. Familiarity with machine learning or deep learning concepts, with an interest in applying these methods to genomic data analysis 5. Experience or strong interest in working with large genomic datasets, reference genomes, and high-performance computing environments
106. Vascular development
Supervisor: Sebastien Gauvrit
University: University of Saskatchewan (Saskatoon campus)
Our research project investigates the role of specific genes in vascular development and disease. We use CRISPR-Cas9–mediated genome editing to modify candidate genes precisely and assess their function in vivo using zebrafish models, which enable real-time imaging of vascular formation. These studies are complemented by cell culture approaches to examine molecular and cellular mechanisms in a controlled environment. Together, this integrated strategy allows us to define how genetic perturbations influence vascular biology and contribute to disease processes.
Research area, student roles & skills
Research area: Our lab studies the genetic and molecular mechanisms that regulate vascular development and function, with a particular focus on how disruptions in these processes lead to human vascular diseases. Using complementary zebrafish and mouse genetic models, we identify and manipulate key genes and signalling pathways involved in blood/lymphatic vessel formation. This work aims to define the origins and progression of vascular disorders and to inform the development of targeted therapeutic strategies, with a particular interest in brain vascular biology.
Student roles: The student will contribute to ongoing research projects by assisting in the investigation of gene function during vascular development. This will include performing molecular biology experiments such as genotyping, basic cloning, and supporting CRISPR-Cas9–based genome editing workflows. The student will also participate in zebrafish husbandry, embryo collection, microinjection, and phenotypic analysis using microscopy. In addition, the student will work with cell culture systems to support functional assays and help analyse experimental outcomes. The role involves maintaining accurate laboratory records, organizing data, and contributing to data interpretation and presentation. The student is expected to actively engage in lab meetings, collaborate with other lab members, and progressively take ownership of aspects of the project as their skills develop.
Skills required: The student should have a background in biology, biochemistry, or a related life sciences field, with an interest in genetics, developmental biology, or vascular biology. Prior experience in basic molecular biology techniques (e.g., PCR, cloning, or genotyping), familiarity with CRISPR-Cas9 genome editing and/or experience working with model organisms such as zebrafish would be an asset, but not required. The student should demonstrate strong organizational skills, attention to detail, and the ability to work both independently and as part of a team. An interest in microscopy, imaging, and data analysis is beneficial. Excellent communication skills and a willingness to learn
107. Viral regulation of chromatin
Supervisor: Kristen Conn
University: University of Saskatchewan (Saskatoon campus)
ICP4 is the only essential HSV1 transcription activator. It is therefore absolutely critical for the successful takeover of the host cell and the production of progeny HSV1 virions. We have identified that ICP4 destabilizes chromatin by increasing the chromatin exchange of histones (histone dynamics). The regulation of histone dynamics is a novel activity for a viral protein that has never been described before. How ICP4 regulates histone dynamics is not known. It is also now known how ICP4- regulation of histone dynamics relates to its mechanisms to activate HSV1 gene expression.
As a first step to characterize how ICP4 regulates histone dynamics, we aim to identify ICP4-interacting proteins using proximity ligation (BioID). This method uses a biotin ligase (BirA) fused to the protein of interest to biotinylate proteins within a small distance from the protein of interest. The biotinylated proteins are then purified, identified by mass spectrometry, and tested for their functional roles. This method will enable the identification of proteins that directly, indirectly, or transiently interact with ICP4, and it has never before been used for ICP4.
By identifying what nuclear proteins interact with ICP4, we can test their functional relevance for ICP4 regulation of histone dynamics and begin to characterize this activity and how it relates to HSV1 nuclear takeover.
Research area, student roles & skills
Research area: We study the interactions between viruses and host cells. Specifically, we investigate how viruses attempt to take over the cell nucleus and how the cell attempts to prevent this takeover. We use herpes simplex virus (HSV1) and related veterinary alphaherpesviruses to investigate the roles of chromatin in this "battle for the nucleus". We have identified a novel chromatin regulatory function for the only essential HSV1 transcription activator, ICP4. We aim to characterize this function and investigate how ICP4 might regulate chromatin to activate viral gene expression and promote viral takeover of the cell nucleus.
Student roles: The student will be directed and trained in all techniques and background knowledge required to conduct their research project. They will independently engage in their project with oversight, supervision, and mentorship from the PI and laboratory technician. The student will meet with the PI weekly to review their progress, evaluate results, address any arising issues, and plan the research activities for the next week. They will also participate and present in weekly laboratory group meetings and events hosted by the Department of Veterinary Microbiology. The student will present their research project and results at the annual Western College of Veterinary Medicine Undergraduate Research Poster Day. Towards identification of ICP4-interacting proteins, the student will establish and optimize a proximity ligation protocol to isolate ICP4-interacting proteins. The student will be responsible to construct and characterize a stable cell line with inducible expression of a BirA-ICP4 fusion protein. The student will verify functionality of the BirA-ICP4 fusion protein using viral complementation assays. The student will then optimize biotinylation conditions in cells that express BirA-ICP4 , or BirA as a control. The student will be responsible to maintain up-to-date and accurate records of their research activities and data analyses. They will analyze, interpret, and synthesize the data they acquire (with guidance) and prepare figures to best present their results.
Skills required: The student should have basic knowledge in the areas of cell biology and microbiology with some experience in common molecular biology techniques, including micropipetting. Practical experience with tissue culture techniques would be an asset, but is not required. The student should have excellent oral and written English communication abilities and possess strong problem-solving, organization, and interpersonal skills. The student should have a positive attitude, willingness to self-asses and improve, and take initiative in their learning and research activities.
108. Viral transcription factor dynamics
Supervisor: Kristen Conn
University: University of Saskatchewan (Saskatoon campus)
Chromatin regulates gene expression by regulating the access of the transcription machinery to the DNA. Gaining (and maintaining) access to DNA that is assembled in chromatin is a critical step for transcription.
How ICP4-regulates transcription of HSV1 genes assembled in chromatin is not yet fully understood. The ICP4 DNA-binding domain binds to sequence-specific DNA sites. However, binding to DNA is generally considered non-essential for ICP4 to activate transcription. Instead, current models propose that ICP4 interacts with other cellular transcription factors and transcription co-activator proteins to bridge the promoter and terminator elements of ICP4-regulated genes. This “gene-looping” mechanism is suggested to increase the rate of RNA polymerase recycling onto ICP4-regulated gene promoters. This model suggests that ICP4 is stably located at ICP4-regulated gene promoters to establish and maintain the “gene loop”. Unfortunately, this model does not consider how ICP4 or other cellular transcription factors access the regulatory elements of ICP4-regulated genes in the presence of viral chromatin.
To further our understanding of ICP4-regulated transcription, we aim to evaluate the nuclear mobility of ICP4 in live cells. If ICP4 stably binds to DNA (or chromatin) with high affinity, it would be expected to have slower nuclear mobility than if it engages in low-affinity binding events. The observed mobility of ICP4 will be related to the described mobilities of cellular transcription factors with well-characterized transcription regulation mechanisms (such as FoxA and Sox9). This information will increase our knowledge of ICP4 functions to enable further testing of the proposed model for ICP4-regulated transcription and support characterization of the mechanisms to overcome the chromatin barrier to access ICP4-regulated genes.
Research area, student roles & skills
Research area: We study the interactions between viruses and host cells. Specifically, we investigate how viruses attempt to take over the cell nucleus and how the cell attempts to prevent this takeover. We use herpes simplex virus (HSV1) and related veterinary alphaherpesviruses to investigate the roles of chromatin in this "battle for the nucleus". We have identified a novel chromatin regulatory function for the only essential HSV1 transcription activator, ICP4. We aim to characterize this function and investigate how ICP4 might regulate chromatin to activate viral gene expression and promote viral takeover of the cell nucleus.
Student roles: The student will be directed and trained in all techniques and background knowledge required to conduct their research project. They will independently engage in their project with oversight, supervision, and mentorship from the PI and laboratory technician. The student will meet with the PI weekly to review their progress, evaluate results, address any arising issues, and plan the research activities for the next week. They will also participate and present in weekly laboratory group meetings and events hosted by the Department of Veterinary Microbiology. The student will present their research project and results at the annual Western College of Veterinary Medicine Undergraduate Research Poster Day. To evaluate the nuclear mobility of ICP4, or a functionally inactive ICP4 mutant, the student will use live cell confocal imaging techniques. We already have within the laboratory expression constructs that encode wild-type or mutant ICP4- red fluorescent protein fusion proteins. The student will be responsible to create expression plasmids that encode wild-type or mutant ICP4 fused to a green-to-red photo-convertible fluorescent protein. These constructs will be transfected into mammalian cells to examine ICP4 nuclear mobility and the dynamics of ICP4 exchange in the presence or absence of HSV1 infection. The student will be responsible to maintain up-to-date and accurate records of their research activities and data analyses. They will analyze, interpret, and synthesize the data they acquire (with guidance) and prepare figures to best present their results.
Skills required: The student should have basic knowledge in the areas of cell biology and microbiology with some experience in common molecular biology techniques, including micropipetting. Practical experience with tissue culture techniques would be an asset, but is not required. The student should have excellent oral and written English communication abilities and possess strong problem-solving, organization, and interpersonal skills. The student should have a positive attitude, willingness to self-asses and improve, and take initiative in their learning and research activities.
109. eDNA Laurentides lakes
Supervisor: Malgorzata Gazda
University: Université de Montréal
Location: Montreal, Québec
Start date: 2027-05-03 (flexible)
Disciplines: Biological Sciences, Biology, Genetics, Computer Science, Molecular Biology, Veterinary Science and Medicine
Molecular techniques revolutionized modern biology. The eDNA sampling nowadays became a powerful method in order to monitor the species biodiversity. The sampling was done in 2024 as a part of global eDNA lake sampling aiming to provide insights about lake diversity at the global scale. We will investigate consistency of ecological data collected from 5 lakes around the Station de biologie des Laurentides. We will evaluate possibility of assesing lake condidtion with eDNA sampling and correlate it with environmental factors. Ultimate aim of the project is to investigate if just with sampling top layer of the water of the lake we are able to recover the species information in the lake. If the analysis will give negative results the further field sampling will be designed in order to design the most comprehensive way of eDNA sampling.
Research area, student roles & skills
Research area: In my research group we combine population genomics, classical genetics, biochemistry, physiology, molecular biology, phylogenetics, neurobiology and behavior. The interdisciplinarity is achieved thanks to wide range of collaborations as well as our willingness and courage to explore unknown. We work on wide variety of organisms from birds to plants, with main model species of canary birds. Other than genotype phenotype association we are also interested in understanding how gene function and expression evolve across species as well as how is it impacted by organismal biology, physiology and environment.
Student roles: Candidate depending on their experience and interest will be able to develop the bioinformatics skills, by managing the sequencing data, analysis of eDNA data, already available in the lab (sampling done 05/2024). Additionally there will be opportunity to learn practical skills in molecular biology lab, such as extracting DNA, preparing sequencing libraries. There is also potential for additional field work and gaining practical skills in eDNA sampling. Intern will be expected to write a report based on their projects as well as present it at the group meeting. Potentially the mix of both, so the computer analysis combined with the bioinformatics is also possible.
Skills required: Candidate for the internship should be interested in evolutionary biology. Basics of scripting/basic programming will be beneficial but not required. General understanding of evolutionary biology, genetics and genomics or computer science will be helpful in order to work in an interdisciplinary team with background in biology and computer science. The interest in freshwater ecosystems and/or eDNA studies will be a plus. Student should be willing to work collaboratively as a part of the team, with professionalism, attention to details and eager to learn.